LOCUS       sequence-299822-        1115 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression and labeling of Drp1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Abrisch RG, Gumbin SC, Wisniewski BT, Lackner LL, Voeltz GK
  TITLE     Fission and fusion machineries converge at ER contact sites to 
            regulate mitochondrial morphology.
  JOURNAL   J Cell Biol. 2020 Apr 6;219(4). pii: 133843. doi: 
            10.1083/jcb.201911122.
  PUBMED    32328629
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2020-04-6"; volume: "219(4). pii: 133843. doi"; 
            pages: " 10.1083/jcb.201911122"
COMMENT     Sequence Label: SNAP-Drp1
FEATURES             Location/Qualifiers
     source          1..1115
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     gap             2..19
                     /estimated_length=18
     regulatory      66..75
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             72..617
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
ORIGIN
        1 cnnnnnnnnn nnnnnnnnng cngagctggt ttagtgaacc gtcagatccg ctagcgctac
       61 cggtcgccac catggacaaa gactgcgaaa tgaagcgcac caccctggat agccctctgg
      121 gcaagctgga actgtctggg tgcgaacagg gcctgcaccg tatcatcttc ctgggcaaag
      181 gaacatctgc cgccgacgcc gtggaagtgc ctgccccagc cgccgtgctg ggcggaccag
      241 agccactgat gcaggccacc gcctggctca acgcctactt tcaccagcct gaggccatcg
      301 aggagttccc tgtgccagcc ctgcaccacc cagtgttcca gcaggagagc tttacccgcc
      361 aggtgctgtg gaaactgctg aaagtggtga agttcggaga ggtcatcagc tacagccacc
      421 tggccgccct ggccggcaat cccgccgcca ccgccgccgt gaaaaccgcc ctgagcggaa
      481 atcccgtgcc cattctgatc ccctgccacc gggtggtgca gggcgacctg gacgtggggg
      541 gctacgaggg cgggctcgcc gtgaaagagt ggctgctggc ccacgagggc cacagactgg
      601 gcaagcctgg gctgggtagc ggtagcggtg gcagctctcg agtgatggag gcgctaattc
      661 ctgtcataaa caagctccag gacgtcttca acacggtggg cgccgacatc atccagctgc
      721 ctcaaatcgt cgtagtggga acgcagagca gcggaaagag ctccgtgcta gaaagcctgg
      781 tggggaggga cctgcttccc agaggtactg gaanttgtca cccggagacc tctcattctg
      841 caactggtcc atgtttcaca agaagataaa cggaaaacaa cagagaagaa aatggagtgg
      901 aagcaganaa tggggtaaat ttcttcacac caaaaataag ctttacncgg attttgatga
      961 anntcgacan gaaattgaaa atgaaannna aannanttcn nnaatannnn nntagccctg
     1021 ancnnnntcn tananttttt cncccnnntg tcattnnann ntngngnntt gcnnnngacn
     1081 nnnncngtan gnngatnacn agnanantga nctna
//
