LOCUS       sequence-299850-        1800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For the expression and purification of the truncated TMC1 protein 
            from Chelonia mydas. The protease cleavage site was replaced with 
            the HRV 3C site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Jia Y, Zhao Y, Kusakizako T, Wang Y, Pan C, Zhang Y, Nureki O, 
            Hattori M, Yan Z
  TITLE     TMC1 and TMC2 Proteins Are Pore-Forming Subunits of Mechanosensitive
            Ion Channels.
  JOURNAL   Neuron. 2020 Jan 22;105(2):310-321.e3. doi: 
            10.1016/j.neuron.2019.10.017. Epub 2019 Nov 21.
  PUBMED    31761710
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2019.10.017"; journalName: "Neuron"; date: 
            "2020-01-22- 22"; volume: "105"; issue: "2"; pages: "310-321.e3"
COMMENT     Sequence Label: GFP-CmTMC1truncated
FEATURES             Location/Qualifiers
     source          1..1800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(149..160)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 aaattttacg cctacaaagt tatgatgaca aagaagtgga tgaaattcca gcgagatttc
       61 gagaatttca agaccgcttg catcccatgg gaaatgaaga taaaggaaat cgaatctcat
      121 ttcggatctt ctgttgcatc ttacttcatc ttccttcgat ggatgtacgg aatcaacatg
      181 atcctatttg gccttacatt cggacttgtt atggttccag aagttcttat gggaaagcct
      241 tatggaacac ttccacgaaa gaccgtccct cggggcgagg agcgaacagc aatgaacttc
      301 gcaacacttt gggatttctc tggacttgca cagtactctg ttctcttcta cggctactac
      361 aacaaccagc gaacaatcgg atggcttaaa ttccgaatgc cactttctta cttccttgtt
      421 ggagttggaa caatcgcata ctcttacatg gttgttatcc gaacaatggc acgaaacgca
      481 aatgaggagg gtggtggtga tgatacatct ttcaacttct cttggaagat gtttacatct
      541 tgggattacc ttatcggaaa cccagaaaca gcagataaca aattcgcatc tatcacaaca
      601 tctttcaaag aagcaatcgt tgaagaacag gagaatcgga aggaggagaa catccatctt
      661 acacgattcc ttcgagttct tgcaaacttc cttgcacttt gcacacttgc aggatctgga
      721 taccttatct acttcgttgt tcgacgatct cagaaattcg cacttgaagg acttgataac
      781 tacggatggt gggaacgaaa cgaagttaac atggttatgt ctcttcttgg aatgttctgc
      841 ccaacactat ttgacgttat ctcttctctt gagaattatc atccacgaat cgcacttcga
      901 tggcagcttg gacgaatctt cgcactattt ctgggaaacc tttacacatt catcatcgca
      961 cttatggatg aaatcaacct taaactagag gaggagaaga tcgttaaata caacatgaca
     1021 atctgggaag catctcttta caacggaaca atctctgaga atgctacagc accaccaatc
     1081 caggttgatc cagcagatat cccacgagga ccatgctggg aaacaatggt tggacaggag
     1141 ttcgttcgac ttacaatctc tgatacaatg acaacataca tcacaatcat catcggagat
     1201 ttccttcgag cagtgtttgt gcgattcttc aactactgct ggtgctggga tcttgaatac
     1261 ggattcccat cttactctga gttcgatatc tctggaaacg ttcttggact tatcttcaac
     1321 cagggaatga tctggatggg atctttctac gcaccatgcc ttccagcaat caacgtgttt
     1381 cggcttcata catctatgta ccttcagtgc tgggcagtta tgtgctgcaa cgttccacat
     1441 gaacgagtat ttaaggcatc tcgatctaac aacttctaca tggcaatgtt actgtttatc
     1501 ctatttctgt ctacacttcc agcagtttac acaatcgttt ctatcccacc atctttcgat
     1561 tgcggaccat tctctggaaa gactaggatg ttcgaagtta tctctgaaac acttgagaat
     1621 gacttcccat cttggttcgg aaaggtcttc agttacgcat ctaacccagg acttatcctt
     1681 ccattcatcc ttcttatggt tctttctatc tactacctta acgcaacatc taaatcttac
     1741 aaagaagcaa accttgactt aaagaagaaa cttcagagtc aggcagaaga gaataagtaa
//
