LOCUS       sequence-299852-        1536 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For the expression and purification of the truncated TMC2 protein 
            from Melopsittacus undulates. The protease cleavage site was 
            replaced with the HRV 3C site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Jia Y, Zhao Y, Kusakizako T, Wang Y, Pan C, Zhang Y, Nureki O, 
            Hattori M, Yan Z
  TITLE     TMC1 and TMC2 Proteins Are Pore-Forming Subunits of Mechanosensitive
            Ion Channels.
  JOURNAL   Neuron. 2020 Jan 22;105(2):310-321.e3. doi: 
            10.1016/j.neuron.2019.10.017. Epub 2019 Nov 21.
  PUBMED    31761710
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2019.10.017"; journalName: "Neuron"; date: 
            "2020-01-22- 22"; volume: "105"; issue: "2"; pages: "310-321.e3"
COMMENT     Sequence Label: GFP-MuTMC2truncated
FEATURES             Location/Qualifiers
     source          1..1536
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(146..157)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ctttacgcat acaagatgat gatggcaaag aagtgggtaa agtttaagcg agatttcgat
       61 aacttcaaga cccaatgcat cccatgggaa atgaagataa aggaagttga atctcatttc
      121 ggatcttctg ttgcatctta cttcatcttc cttcgatgga tgtacggagt taaccttgtt
      181 ctatttggtc ttatcttcgg acttgtaatt atacctgaga gatcttctaa actttctcag
      241 atgtcttctg gaacatggct ttacttcgaa caggaagcat ggcttgatga tcttcagcga
      301 atggcacgaa acgcaaacga atctacagca gatggagatg atgataactt catcttctct
      361 tggaagatgt ttacatcttg ggattacctt atcggaaacc cagaaacagc agataacaaa
      421 ttcgcatcta tcacaacatc tttcaaagaa tctatcgttg atgaacagga atctaacaaa
      481 gatgagaata tacatcttcg acgattcctt cgagttcttg caaacgttct tatcatctgc
      541 tgcctttgcg gatctggata ccttatctac ttcgttgtta aacgatctca gacattctct
      601 aagatgcaga atgtcggatg gtacgaacga aacgaagttg aaatcgttat gtctcttctt
      661 ggaatgttct gcccaccatt atttgagaca atcgcagcac ttgagaatta tcatccacga
      721 atcggactta aatggcagct tggacgaatc ttcgcactgt tcctaggtaa cctttacaca
      781 ttccttcttg cacttatgga tgatgttaac gagaagctcg cggaagaaga tgttgttaag
      841 aatattacac attggacact tcttggacat cataactctt ctatgggaaa tagttcgagc
      901 gctactccac cgcctcttga tccagcagat gttccacgag gaccatgctg ggaaacaaca
      961 gttggaatcg agttcgttcg acttacagtt tctgatatcc ttgttacata catcacaatc
     1021 cttgttggag atttcatccg agcatgcttc gttcgattcg ttaactactg ctggtgctgg
     1081 gatcttgaag caggattccc atcttacgca gagttcgata tctctggaaa cgttcttgga
     1141 cttgtattta atcagggaat gatctggatg ggatctttct acgcaccagg acttgttgga
     1201 atcaacgttc ttcgacttct tacatctatg tacttccagt gctgggcagt tatgtcttct
     1261 aacgttccac atgaacgagt gtttaaggca tctaaatcta acaacttcta catgggactt
     1321 cttcttctta tccttttcct ttctcttctt ccagttgcat acacaatcat gtctcttcca
     1381 ccatctttcg attgcggacc attctctgga aagaagcgta tgtacgaagt tatccaggaa
     1441 acaatcgaac ttgatttccc actgtttgtc gcaaagatct tctcttacgt tgcaaaccca
     1501 ggattgataa tacccgctat tcttcttatg gtttaa
//
