LOCUS       sequence-29998-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Krupka N, Strappe P, Gotz J, Ittner LM
  TITLE     Gateway-compatible lentiviral transfer vectors for ubiquitin 
            promoter driven expression of fluorescent fusion proteins.
  JOURNAL   Plasmid. 2010 May;63(3):155-60. doi: 10.1016/j.plasmid.2010.01.002. 
            Epub 2010 Feb 2.
  PUBMED    20132838
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.plasmid.2010.01.002"; journalName: "Plasmid"; date: 
            "2010-05"; volume: "63"; issue: "3"; pages: "155-60"
COMMENT     Sequence Label: pLVU/GFP
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    63..187
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(228..533)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(330..349)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 tgctcaccat ttcgaaccgc gggccctcta gactcgagcg gccgccactg tgctggatat
       61 caaccacttt gtacaagaaa gctgaacgag aaacgtaaaa tgatataaat atcaatatat
      121 taaattagat tttgcataaa aaacagacta cataatactg taaaacacaa catatccagt
      181 cactatggtc gacctgcaga ctggctgtgt ataagggagc ctgacattta tattccccag
      241 aacatcaggt taatggcgtt tttgatgtca ttttcgcggt ggctgagatc agccacttct
      301 tccccgataa cggagaccgg cacactggcc atatcggtgg tcatcatgcg ccagctttca
      361 tccccgatat gcaccaccgg gtaaagttca cgggagactt tatctgacag cagacgtgca
      421 ctggccaggg ggatcaccat ccgtcgcccg ggcgtgtcaa taatatcact ctgtacatcc
      481 acaaacagac gataacggct ctctctttta taggtgtaaa ccttaaactg catttcacca
      541 gcccctgttc tcgtcagcaa aagagccgtt catttcaata aaccgggcga cctcagccat
      601 cccttcctga ttttccgctt tccagcgttc ggcacgcaga cgacgggctt cattctgcat
      661 ggttgtgctt accagaccgg agatattgac atcatatatg ccttgagcaa ctgatagctg
      721 tcgctgtcaa ctgtcactgt aatacgctgc ttcatagcat acctcttttt gacatacttc
      781 gggtatacat atcagtatat attcttatac cgcaaaaatc agcgcgcaaa tacgcatact
      841 gttatctggc ttttagtaag ccggatccag atctttacgc cccgccctg
//
