LOCUS       sequence-300-exp        3488 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1999
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLT61
FEATURES             Location/Qualifiers
     source          1..3488
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        19..37
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     95..111
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     95..111
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(879..895)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(880..896)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(921..939)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(949..966)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(949..965)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(958..980)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1107..1562
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1194..1213)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1404..1425
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1588..1692
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1693..1761,1762..2553)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1911..1930)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2724..3312
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3213..3232
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3466..3483
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aacctggctt atcgaaatta atacgactca ctatagggag accggcagat ctgatatcat
       61 cgatgaattc gagctccacc gcggtggcgg ccgctctaga actagtggat cccccccgag
      121 cacaatgacc tcaacggaat gtttgtcgct tccagaagcg ttggtagcag taagagtgta
      181 caaaccagca tgcttgcgag ttgctccacg gagcacaaat cgggtcttgt agtcttcgct
      241 ttcaatcctg atcttttgat catcgactgg tttctcattg aagatccatt ccttcttcgg
      301 tggtggttct ccgcgtactg gaacatcgaa ttccacatcg tttcctacct tgatagtgat
      361 cgtcttcatt gcttcacgat caatccatgg cttcaagttt cttggctttg caacaacctt
      421 tcggcttggg tctgaagcct ctcccggtcc ggccttgtta acagccttca cacggaactg
      481 gtactcttct ccctccttga gtccaagaat ctcagcattg gtttgatctc cagaaacctt
      541 tccgcattct tgccagtctc ttccatgttt gcccttcttc tcaatgacgt attgagtgat
      601 tggagctcct ccgtcagact ttggtggttc ccattcaagt gagacacgat cagcatccca
      661 gtcgacaaca tccggagttc cggtttttcc tggctcatca tatggattct tggctttggt
      721 gtccttatca gtagacaatg catcagaagc tccttcctta ttgacagcct tgacacggaa
      781 ctgatatgtt tgtcccttct tgagaccatc aatatgagcc ttcgtatcct taactttggc
      841 gcatggaacc catcttccga attcgatatc aagcttatcg ataccgtcga cctcgagggg
      901 gggcccggta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
      961 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     1021 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     1081 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
     1141 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     1201 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     1261 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     1321 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     1381 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     1441 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     1501 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     1561 ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     1621 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     1681 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     1741 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     1801 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     1861 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     1921 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     1981 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     2041 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     2101 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     2161 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     2221 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     2281 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     2341 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     2401 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     2461 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     2521 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     2581 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     2641 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     2701 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     2761 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     2821 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     2881 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     2941 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     3001 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     3061 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     3121 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     3181 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     3241 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     3301 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     3361 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     3421 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     3481 gaggaagc
//
