LOCUS       sequence-30003-e         632 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 632)
  TITLE     George Cross Lab Plasmids
REFERENCE   2  (bases 1 to 632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLEW82v4
FEATURES             Location/Qualifiers
     source          1..632
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    208..226
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    234..252
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    260..278
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(284..303)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(285..303)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 atggcgccgg gcctttcttt atgtttttgg cgtcttccat tttaccaaca gtaccggaat
       61 gctaatgtca ctgcgagcga cattcaagaa tgtaacacaa aggaattcca agcttgtgaa
      121 ttttactttt tggtgtaatt gaagtcttca agttgaaaaa aatggggaaa aggaaaaaaa
      181 actcgaaggc gcgtgcagcc cgggagatct ctatcactga tagggagcta ccgtctctat
      241 cactgatagg gagcggatgt ctctatcact gatagggaga tctccctata gtgagtcgta
      301 ttaatcagcc ggtgtgttgc caaagacatt ctgtataatt ttccaactac cctaacctga
      361 ttcactcatc gttgagcatt tgttcttcta ctttttgacc gcaagtgtcg ttattagtat
      421 tcggtcctcc gcggcgaggc gaatcgctca gtagtgttag tgcaggcatt gtttctcttt
      481 tccatttcgg ctcgctctct gtgccagaaa ggcttttgga gtcttggtta ctcgtgggac
      541 tagggcaagc gtcgcttccc taataattgt tgctaacgtt tcaccgtgcg tgtgtggata
      601 tcttccaagt tgtcattttc tctacccctc tc
//
