LOCUS       sequence-30007-e         725 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 725)
  TITLE     George Cross Lab Plasmids
REFERENCE   2  (bases 1 to 725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLEW100v5-177-HYG
FEATURES             Location/Qualifiers
     source          1..725
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    234..252
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    255..273
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     550..569
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        550..568
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggcgccgggc ctttctttat gtttttggcg tcttccattt taccaacagt accggaatgc
       61 taatgtcact gcgagcgaca ttcaagaatg taacacaaag gaattccaag cttgtgaatt
      121 ttactttttg gtgtaattga agtcttcaag ttgaaaaaaa tggggaaaag gaaaaaaaac
      181 tcgaaggcgc gtgcagcccg ggacgagaac atgttaaact cagtactcag ggatctctat
      241 cactgatagg gagatctcta tcactgatag ggaaccgtac gccgtaagcg ctacttttac
      301 tgcttaatct ttcctatata agatatattt ctattactat atatactttt ttaactttat
      361 cattattatc ctattgtgtg ttgtattgac tttacaataa aacatattat atctaacatg
      421 tcaaaagcat cggctcttca atacagttac ccgtccctta tcaacacagt acaatatact
      481 ctgtcatgac aataagtata tataagagcc agaatgcacc cgcgctgggt ggaaagctag
      541 gtacctgatt aatacgactc actataggga gatctcccgt accgggggca cagcaaggtc
      601 ttctgaaatt catgtttttt tttttttact ctgcattgca gtctccgctc ttatttagtt
      661 ttgctttncg taaggtctcg ttgctgccat aaaataagct ctagaactag tgatgaaaaa
      721 gcctg
//
