LOCUS       sequence-300239-       10458 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for SARS-CoV-2 S (surface 
            glycoprotein).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10458)
  TITLE     SARS-CoV-2 protein Mammalian expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 10458)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4134320
FEATURES             Location/Qualifiers
     source          1..10458
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        306..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             4402..4422
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             4423..4500
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     polyA_signal    4569..4690
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4580..4599)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4634..4653
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      4805..4851
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      4881..5017
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    5062..5133
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     rep_origin      complement(5359..5947)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5439..5458)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        6447..7656
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     7493..7512
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             7660..8688
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     polyA_signal    8715..8763
                     /note="synthetic polyadenylation signal"
     CDS             join(9029..9097,9098..9889)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9247..9266)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      9902..10038
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    10190..10261
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(10266..10312)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    10330..10378
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catgctgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatca gatctttgtc
      541 gatcctacca tccactcgac acacccgcca gcggccgcca ccatgttcgt ctttcttgtg
      601 ttactccccc tggtttcgtc ccagtgcgtg aacttgacta cgcgaaccca actgccgcca
      661 gcatatacaa atagtttcac ccgtggagta tactaccctg ataaagtgtt tagatcaagc
      721 gtactacact ctactcagga cctgtttctc cccttcttct ccaatgtcac atggtttcat
      781 gcgatccacg ttagcggcac caacgggaca aagcgctttg acaaccctgt gctgccattc
      841 aatgatggtg tgtattttgc ttctaccgag aagagcaaca taattcgggg ctggatcttc
      901 ggaactaccc tcgacagtaa aactcagtca ttgctgattg tgaataacgc cacaaatgtc
      961 gttatcaagg tctgtgaatt tcaattctgc aacgatccat tccttggggt gtattaccat
     1021 aaaaataata agtcctggat ggagagcgaa tttagggtat actcgtctgc caacaattgt
     1081 actttcgagt atgtgtctca gcccttctta atggatctgg aaggtaaaca gggcaacttt
     1141 aagaacctcc gcgagtttgt attcaaaaat atagacggat actttaagat ttattccaag
     1201 cacacaccaa tcaatttggt gcgggatctc ccgcaagggt tcagcgctct ggaacctcta
     1261 gtggacctcc ccatcggcat taacatcaca agattccaga cgctgttagc cctgcataga
     1321 tcatacctta ctcccggtga cagtagttct gggtggaccg caggcgcggc tgcatattac
     1381 gtgggatatt tgcagcctcg gacgtttctg ctgaaataca acgagaacgg caccattaca
     1441 gatgccgtgg attgcgccct tgacccccta agcgaaacta agtgtacgct taaaagcttc
     1501 accgtcgaga agggaattta ccagacttcc aactttaggg tccagccgac agaatcaata
     1561 gttaggttcc caaatatcac caatttgtgc ccttttgggg aagtcttcaa tgctacacga
     1621 tttgcttcgg tttatgcctg gaacaggaag cgtatctcaa attgcgtcgc ggactacagc
     1681 gtgctctata atagtgcaag tttctcgacc tttaaatgtt atggcgtgtc cccgaccaaa
     1741 ctaaatgacc tctgttttac aaacgtttac gccgattctt ttgtgattcg cggtgatgag
     1801 gtacgccaaa tcgcaccggg ccagactggc aagattgccg attacaacta caagttgcct
     1861 gacgacttca ccggatgtgt tatagcctgg aatagcaata atctggattc taaggtcgga
     1921 ggtaactata actacctgta tagactgttc cgtaagtcaa acctgaaacc ctttgagcgg
     1981 gacatctcca cagagattta ccaagcaggg tctaccccct gcaatggggt tgaaggattc
     2041 aattgctact ttccattaca gagctacggg ttccagccta cgaacggggt tggttaccag
     2101 ccttataggg tagtggtgct gtcgttcgaa ctgttgcacg ctccagctac agtgtgcggg
     2161 cctaaaaaat caactaacct ggttaaaaat aagtgtgtta attttaactt caatggctta
     2221 accggaactg gagtgctcac ggagtcaaac aagaaatttc taccgtttca gcaattcggg
     2281 agggatatcg cagacactac ggacgcggtc agagatccac agacccttga gatccttgac
     2341 ataacaccct gctctttcgg aggtgtctcc gtaattacac caggcactaa tacctctaat
     2401 caggtggccg tgctttatca ggatgttaac tgtacagagg tcccggtggc tatccatgcc
     2461 gaccaattga cacctacgtg gcgcgtctat tctaccggct ccaacgtctt ccagacccgg
     2521 gctggatgct tgataggcgc agaacacgta aacaactctt acgaatgtga tattcccatt
     2581 ggagccggga tctgcgcaag ctaccagacc caaacaaaca gtcccagacg ggctcgtagc
     2641 gtggcctctc agagcataat agcctatacc atgtcccttg gcgcggagaa tagtgtggca
     2701 tactcaaata atagcatcgc tatccccact aactttacta tttccgtgac gactgagatt
     2761 ctgccagtgt ctatgactaa gacatccgtg gattgcacaa tgtatatttg tggggattcc
     2821 acagagtgta gcaacttgtt gctgcaatat ggatcattct gtacgcagct gaatcgcgcc
     2881 ctcaccggca tcgctgtgga acaggacaag aacactcaag aggtctttgc tcaggtgaag
     2941 caaatctaca agaccccacc tataaaagac ttcgggggtt ttaacttctc gcagattctc
     3001 cccgacccca gcaagccttc aaaaaggagc tttattgagg acctgttgtt caacaaggtt
     3061 actttagccg atgccggttt tatcaaacag tacggcgact gtcttggcga tatagcagcc
     3121 cgggatctga tttgcgcaca aaaattcaat ggccttaccg tactgccacc ccttctcacc
     3181 gacgaaatga tcgcgcagta cacctccgca ctgctggctg ggaccatcac ttcgggatgg
     3241 acctttgggg ctggtgctgc tttgcaaatt cccttcgcca tgcagatggc ctatcgattc
     3301 aatggcatag gggtaacaca gaacgtattg tatgaaaatc aaaagctaat cgctaaccag
     3361 tttaattcag ccattggtaa gatccaggat tcactctcca gtacagcctc tgccctggga
     3421 aagctccagg acgtggtcaa ccagaacgcg caggcattga atacactcgt caaacagctg
     3481 agtagcaatt tcggcgcaat cagtagtgtc ttaaacgata tactgagtag gcttgataaa
     3541 gtagaggccg aggtccagat tgacagacta atcacggggc gtcttcagag tttacagacc
     3601 tatgtgaccc aacagctgat ccgagcagcg gaaattcgag caagcgcgaa cctggccgct
     3661 actaaaatga gtgagtgcgt gctgggccaa agtaaacgag tcgacttttg cggcaaaggg
     3721 tatcatctca tgtctttccc tcagtccgcc cctcacggtg ttgttttcct ccacgtgaca
     3781 tacgttcccg cgcaggaaaa gaattttact acggcacccg ctatttgcca tgacggaaag
     3841 gctcattttc cacgggaggg agtgttcgtt tcaaacggga cacactggtt tgtaactcag
     3901 cggaactttt atgagcctca aatcatcacg acagacaata ccttcgtgag tggcaactgc
     3961 gatgtagtca taggcattgt gaataacact gtttatgatc cacttcagcc ggaactcgac
     4021 agcttcaaag aggaattgga taagtacttt aaaaatcata ccagtccaga cgtagatctc
     4081 ggcgatatat cgggcattaa cgccagtgtg gtcaatatac aaaaggagat tgacagactg
     4141 aatgaagtag ctaagaacct caacgaatct ctgattgacc tgcaagagct agggaagtac
     4201 gagcagtata tcaaatggcc atggtacata tggctgggtt ttatcgcggg tcttattgcc
     4261 atagtcatgg tgacgattat gttgtgctgt atgacctcct gctgttcttg tttgaaaggc
     4321 tgctgctcct gtggtagctg ttgcaaattc gatgaagatg actctgaacc cgtgttgaaa
     4381 ggcgttaagc tacactacac agaaaacctt tactttcagt cgaagaggcg atggaaaaag
     4441 aattttatcg cggtgagtgc agccaacaga ttcaaaaaga tatcctcgtc tggtgccctc
     4501 taaggatccg caggcctctg ctagcttgac tgactgagat acagcgtacc ttcagctcac
     4561 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     4621 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     4681 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
     4741 ggaggttttt taaagcaagt aaaacctcta caaatgtggt attggcccat ctctatcggt
     4801 atcgtagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gtgcccctcg
     4861 ggccggattg ctatctaccg gcattggcgc agaaaaaaat gcctgatgcg acgctgcgcg
     4921 tcttatactc ccacatatgc cagattcagc aacggatacg gcttccccaa cttgcccact
     4981 tccatacgtg tcctccttac cagaaattta tccttaaggt cgtcagctat cctgcaggcg
     5041 atctctcgat ttcgatcaag acattccttt aatggtcttt tctggacacc actaggggtc
     5101 agaagtagtt catcaaactt tcttccctcc ctaatctcat tggttacctt gggctatcga
     5161 aacttaatta accagtcaag tcagctactt ggcgagatcg acttgtctgg gtttcgacta
     5221 cgctcagaat tgcgtcagtc aagttcgatc tggtccttgc tattgcaccc gttctccgat
     5281 tacgagtttc atttaaatca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     5341 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     5401 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     5461 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     5521 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     5581 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     5641 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     5701 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     5761 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     5821 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     5881 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     5941 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     6001 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     6061 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     6121 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     6181 tgcatttaaa tttccgaact ctccaaggcc ctcgtcggaa aatcttcaaa cctttcgtcc
     6241 gatccatctt gcaggctacc tctcgaacga actatcgcaa gtctcttggc cggccttgcg
     6301 ccttggctat tgcttggcag cgcctatcgc caggtattac tccaatcccg aatatccgag
     6361 atcgggatca cccgagagaa gttcaaccta catcctcaat cccgatctat ccgagatccg
     6421 aggaatatcg aaatcggggc gcgcctggcc tccgcgccgg gttttggcgc ctcccgcggg
     6481 cgcccccctc gtcacggcga gcgctgccac gtcagacgaa gggcgcagga gcgtcctgat
     6541 ccttccgccc ggacgctcag gacagcggcc cgctgctcat aagactcggc cttagaaccc
     6601 cagtatcagc agaaggacat tttaggacgg gacttgggtg actctagggc actggttttc
     6661 tttccagaga gcggaacagg cgaggaaaag tagtcccttc tcggcgattc tgcggaggga
     6721 tctccgtggg gcggtgaacg ccgatgatta tataaggacg cgccgggtgt ggcacagcta
     6781 gttccgtcgc agccgggatt tgggtcgcgg ttcttgtttg tggatcgctg tgatcgtcac
     6841 ttggtgagta gcgggctgct gggctggccg gggctttcgt ggccgccggg ccgctcggtg
     6901 ggacggaagc gtgtggagag accgccaagg gctgtagtct gggtccgcga gcaaggttgc
     6961 cctgaactgg gggttggggg gagcgcagca aaatggcggc tgttcccgag tcttgaatgg
     7021 aagacgcttg tgaggcgggc tgtgaggtcg ttgaaacaag gtggggggca tggtgggcgg
     7081 caagaaccca aggtcttgag cccttcgcta atgcgggaaa gctcttattc gggtgagatg
     7141 ggctgggcac catctgggga ccctgacgtg aagtttgtca ctgactggag aactcggttt
     7201 gtcgtctgtt gcgggggcgg cagttatggc ggtgccgttg ggcagtgcac ccgtaccttt
     7261 gggagcgcgc gccctcgtcg tgtcgtgacg tcacccgttc tgttggctta taatgcaggg
     7321 tggggccacc tgccggtagg tgtgcggtag gcttttctcc gtcgcaggac gcagggttcg
     7381 ggcctagggt aggctctcct gaatcgacag gcgccggacc tctggtgagg ggagggataa
     7441 gtgaggcgtc agtttctttg gtcggtttta tgtacctatc ttcttaagta gctgaagctc
     7501 cggttttgaa ctatgcgctc ggggttggcg agtgtgtttt gtgaagtttt ttaggcacct
     7561 tttgaaatgt aatcatttgg gtcaatatgt aattttcagt gttagacttg taaattgtcc
     7621 gctaaattct ggccgttttt ggcttttttg ttagacaaca tgggtaaaaa gcctgaactc
     7681 accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg
     7741 cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat
     7801 gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac
     7861 tttgcatccg ccgcgctccc gattccggaa gtgcttgaca ttggggagtt cagcgagagc
     7921 ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc
     7981 gaactgcccg ctgttctgca gccggtcgcg gaggcaatgg atgccatcgc tgccgccgat
     8041 cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca
     8101 tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg
     8161 gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag
     8221 gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg
     8281 gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa
     8341 tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg
     8401 cgctacttcg agcggaggca tccggagctt gcaggatcgc cccggctccg ggcgtatatg
     8461 ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca
     8521 gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt
     8581 acacaaatcg cccgcagaag cgccgccgtc tggaccgatg gctgtgtaga agtactcgcc
     8641 gatagtggaa accgacgccc cagcactcgt ccgagggcaa aggaataagc tagtatgtaa
     8701 gcctagtctt agataataaa atcgctatcc atcgaagatg gatgtgtgtt ggttttttgt
     8761 gtgtgtaacg ctaggcgcgc ctggtgtacc gagaacgatc ctctcagtgc gagtctcgac
     8821 gatccatatc gttgcttggc agtcagccag tcggaatcca gcttgggacc caggaagtcc
     8881 aatcgtcaga tattgtactc aagcctggtc acggcagcgt accgatctgt ttaaacctag
     8941 atattgatag tctgatcggt caacgtataa tcgagtccta gcttttgcaa acatctatca
     9001 agagacagga tcagcaggag gctttcgcat gagtattcaa catttccgtg tcgcccttat
     9061 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     9121 aaaagatgct gaagatcagt tgggtgcgcg agtgggttac atcgaactgg atctcaacag
     9181 cggtaagatc cttgagagtt ttcgccccga agaacgcttt ccaatgatga gcacttttaa
     9241 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     9301 ccgcatacac tattctcaga atgacttggt tgagtattca ccagtcacag aaaagcatct
     9361 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     9421 tgcggccaac ttacttctga caacgattgg aggaccgaag gagctaaccg cttttttgca
     9481 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     9541 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacct tgcgtaaact
     9601 attaactggc gaactactta ctctagcttc ccggcaacag ttgatagact ggatggaggc
     9661 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     9721 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     9781 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     9841 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac cgattctagg
     9901 tgcattggcg cagaaaaaaa tgcctgatgc gacgctgcgc gtcttatact cccacatatg
     9961 ccagattcag caacggatac ggcttcccca acttgcccac ttccatacgt gtcctcctta
    10021 ccagaaattt atccttaaga tcccgaatcg tttaaactcg actctggctc tatcgaatct
    10081 ccgtcgtttc gagcttacgc gaacagccgt ggcgctcatt tgctcgtcgg gcatcgaatc
    10141 tcgtcagcta tcgtcagctt acctttttgg cagcgatcgc ggctcccgac atcttggacc
    10201 attagctcca caggtatctt cttccctcta gtggtcataa cagcagcttc agctacctct
    10261 caattcaaaa aacccctcaa gacccgttta gaggccccaa ggggttatgc tatcaatcgt
    10321 tgcgttacac acacaaaaaa ccaacacaca tccatcttcg atggatagcg attttattat
    10381 ctaactgctg atcgagtgta gccagatcta gtaatcaatt acggggtcat tagttcatag
    10441 cccatatatg gagttccg
//
