LOCUS       sequence-30024-e         680 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 680)
  TITLE     Plasmids for Tagging Genes In-situ
REFERENCE   2  (bases 1 to 680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMOTag43M-crelox-HYG
FEATURES             Location/Qualifiers
     source          1..680
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             32..61
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             74..103
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             116..145
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    complement(179..212)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ccccctcgag ggcggccgat cgagatccga ggagcagaag ctgatctcgg aggaggatct
       61 gctgagatcc gaggagcaga agctgatctc ggaggaggat ctgctgagat ccgaggagca
      121 gaagctgatc tcagaggagg atctgctgtg atctagtgcg gccgtcgacg ataagcttat
      181 aacttcgtat agcatacatt atacgaagtt ataccggtac acgttctcgt cggccggccg
      241 ctgcacgcgc cttcgagttt tttttccttt tccccatttt tttcaacttg aagacttcaa
      301 ttacaccaaa aaataaaatt cacaaacttg gtattccttt gtgttacatt cttgatcgct
      361 cgcactgaca ttacagatct tactgttggt aaatgatgaa aaagcctgaa ctcaccgcga
      421 cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct
      481 cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc
      541 gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat
      601 cggccgcgct cccgattccg gaagtgcttg acattgggga attcagcgag agcctgacct
      661 attgcatctc cccgccgtgc
//
