LOCUS       sequence-300251-        3795 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning of TARDBP-A315T.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Pourhaghighi R, Ash PEA, Phanse S, Goebels F, Hu LZM, Chen S, Zhang 
            Y, Wierbowski SD, Boudeau S, Moutaoufik MT, Malty RH, Malolepsza E, 
            Tsafou K, Nathan A, Cromar G, Guo H, Abdullatif AA, Apicco DJ, 
            Becker LA, Gitler AD, Pulst SM, Youssef A, Hekman R, Havugimana PC, 
            White CA, Blum BC, Ratti A, Bryant CD, Parkinson J, Lage K, Babu M, 
            Yu H, Bader GD, Wolozin B, Emili A
  TITLE     BraInMap Elucidates the Macromolecular Connectivity Landscape of 
            Mammalian Brain.
  JOURNAL   Cell Syst. 2020 Apr 22;10(4):333-350.e14. doi: 
            10.1016/j.cels.2020.03.003.
  PUBMED    32325033
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2020.03"; journalName: "Cell Syst"; date: 
            "2020-04-22- 22"; volume: "10"; issue: "4"; pages: "333-350.e14"
COMMENT     Sequence Label: pENTR-TARDBP-A315T
FEATURES             Location/Qualifiers
     source          1..3795
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1920..2019)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2036..2055)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2037..2055)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2060..2076)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2060..2076)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2178..2197)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2189..2998
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2256..2275)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3145..3733
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3634..3653
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatgtctg aatatattcg ggtaaccgaa gatgagaacg atgagcccat
      721 tgaaatacca tcggaagacg atgggacggt gctgctctcc acggttacag cccagtttcc
      781 aggggcgtgt gggcttcgct acaggaatcc agtgtctcag tgtatgagag gtgtccggct
      841 ggtagaagga attctgcatg ccccagatgc tggctgggga aatctggtgt atgttgtcaa
      901 ctatccaaaa gataacaaaa gaaaaatgga tgagacagat gcttcatcag cagtgaaagt
      961 gaaaagagca gtccagaaaa catccgattt aatagtgttg ggtctcccat ggaaaacaac
     1021 cgaacaggac ctgaaagagt attttagtac ctttggagaa gttcttatgg tgcaggtcaa
     1081 gaaagatctt aagactggtc attcaaaggg gtttggcttt gttcgtttta cggaatatga
     1141 aacacaagtg aaagtaatgt cacagcgaca tatgatagat ggacgatggt gtgactgcaa
     1201 acttcctaat tctaagcaaa gccaagatga gcctttgaga agcagaaaag tgtttgtggg
     1261 gcgctgtaca gaggacatga ctgaggatga gctgcgggag ttcttctctc agtacgggga
     1321 tgtgatggat gtcttcatcc ccaagccatt cagggccttt gcctttgtta catttgcaga
     1381 tgatcagatt gcgcagtctc tttgtggaga ggacttgatc attaaaggaa tcagcgttca
     1441 tatatccaat gccgaaccta agcacaatag caatagacag ttagaaagaa gtggaagatt
     1501 tggtggtaat ccaggtggct ttgggaatca gggtggattt ggtaatagca gagggggtgg
     1561 agctggtttg ggaaacaatc aaggtagtaa tatgggtggt gggatgaact ttggtacgtt
     1621 cagcattaat ccagccatga tggctgccgc ccaggcagca ctacagagca gttggggtat
     1681 gatgggcatg ttagccagcc agcagaacca gtcaggccca tcgggtaata accaaaacca
     1741 aggcaacatg cagagggagc caaaccaggc cttcggttct ggaaataact cttatagtgg
     1801 ctctaattct ggtgcagcaa ttggttgggg atcagcatcc aatgcagggt cgggcagtgg
     1861 ttttaatgga ggctttggct caagcatgga ttctaagtct tctggctggg gaatgttgga
     1921 cccagctttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa
     1981 cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg atatccccta
     2041 tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa
     2101 tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc
     2161 ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc
     2221 gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt
     2281 cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt
     2341 tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa
     2401 ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga
     2461 tgcatggtta ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata
     2521 tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc
     2581 gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca
     2641 atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg
     2701 gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt
     2761 cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg
     2821 ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg
     2881 gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat
     2941 tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc
     3001 agaattggtt aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc
     3061 aagctcatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc
     3121 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     3181 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     3241 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     3301 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     3361 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     3421 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     3481 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     3541 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     3601 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     3661 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     3721 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     3781 ttttgctcac atgtt
//
