LOCUS       sequence-300407-        6723 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for SARS-CoV-2 ORF6 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6723)
  TITLE     SARS-CoV-2 protein Mammalian expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 6723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4252988
FEATURES             Location/Qualifiers
     source          1..6723
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      51..97
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_feature    102..173
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(325..461)
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     CDS             complement(join(474..1265,1266..1334))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1097..1116
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     polyA_signal    1600..1648
                     /note="synthetic polyadenylation signal"
     CDS             complement(1675..2703)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     promoter        complement(2707..3916)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(2851..2870)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     rep_origin      4416..5004
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4905..4924
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    5230..5301
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(5346..5482)
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     terminator      complement(5512..5558)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    5673..5794
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5710..5729)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5764..5783
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        complement(6120..6322)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6149..6169)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        6324..6627
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    join(6708..6723,1..33)
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 atcgaagatg gatgtgtgtt ggttttttgt gtgtgtaacg caacgattga tagcataacc
       61 ccttggggcc tctaaacggg tcttgagggg ttttttgaat tgagaggtag ctgaagctgc
      121 tgttatgacc actagaggga agaagatacc tgtggagcta atggtccaag atgtcgggag
      181 ccgcgatcgc tgccaaaaag gtaagctgac gatagctgac gagattcgat gcccgacgag
      241 caaatgagcg ccacggctgt tcgcgtaagc tcgaaacgac ggagattcga tagagccaga
      301 gtcgagttta aacgattcgg gatcttaagg ataaatttct ggtaaggagg acacgtatgg
      361 aagtgggcaa gttggggaag ccgtatccgt tgctgaatct ggcatatgtg ggagtataag
      421 acgcgcagcg tcgcatcagg catttttttc tgcgccaatg cacctagaat cggttaccaa
      481 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
      541 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
      601 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
      661 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatc
      721 aactgttgcc gggaagctag agtaagtagt tcgccagtta atagtttacg caaggttgtt
      781 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
      841 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
      901 tccttcggtc ctccaatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
      961 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     1021 ggtgaatact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     1081 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     1141 ggaaagcgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     1201 atgtaaccca ctcgcgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     1261 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     1321 tgttgaatac tcatgcgaaa gcctcctgct gatcctgtct cttgatagat gtttgcaaaa
     1381 gctaggactc gattatacgt tgaccgatca gactatcaat atctaggttt aaacagatcg
     1441 gtacgctgcc gtgaccaggc ttgagtacaa tatctgacga ttggacttcc tgggtcccaa
     1501 gctggattcc gactggctga ctgccaagca acgatatgga tcgtcgagac tcgcactgag
     1561 aggatcgttc tcggtacacc aggcgcgcct agcgttacac acacaaaaaa ccaacacaca
     1621 tccatcttcg atggatagcg attttattat ctaagactag gcttacatac tagcttattc
     1681 ctttgccctc ggacgagtgc tggggcgtcg gtttccacta tcggcgagta cttctacaca
     1741 gccatcggtc cagacggcgg cgcttctgcg ggcgatttgt gtacgcccga cagtcccggc
     1801 tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat cgaaattgcc
     1861 gtcaaccaag ctctgataga gttggtcaag accaatgcgg agcatatacg cccggagccg
     1921 gggcgatcct gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct gctccataca
     1981 agccaaccac ggcctccaga agaagatgtt ggcgacctcg tattgggaat ccccgaacat
     2041 cgcctcgctc cagtcaatga ccgctgttat gcggccattg tccgtcagga cattgttgga
     2101 gccgaaatcc gcgtgcacga ggtgccggac ttcggggcag tcctcggccc aaagcatcag
     2161 ctcatcgaga gcctgcgcga cggacgcact gacggtgtcg tccatcacag tttgccagtg
     2221 atacacatgg ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt attgaccgat
     2281 tccttgcggt ccgaatgggc cgaacccgct cgtctggcta agatcggcgg cagcgatggc
     2341 atccattgcc tccgcgaccg gctgcagaac agcgggcagt tcggtttcag gcaggtcttg
     2401 caacgtgaca ccctgtgcac ggcgggagat gcaataggtc aggctctcgc tgaactcccc
     2461 aatgtcaagc acttccggaa tcgggagcgc ggcggatgca aagtgccgat aaacataacg
     2521 atctttgtag aaaccatcgg cgcagctatt tacccgcagg acatatccac gccctcctac
     2581 atcgaagctg aaagcacgag attcttcgcc ctccgagagc tgcatcaggt cggagacgct
     2641 gtcgaacttt tcgatcagaa acttctcgac agacgtcgcg gtgagttcag gctttttacc
     2701 catgttgtct aacaaaaaag ccaaaaacgg ccagaattta gcggacaatt tacaagtcta
     2761 acactgaaaa ttacatattg acccaaatga ttacatttca aaaggtgcct aaaaaacttc
     2821 acaaaacaca ctcgccaacc ccgagcgcat agttcaaaac cggagcttca gctacttaag
     2881 aagataggta cataaaaccg accaaagaaa ctgacgcctc acttatccct cccctcacca
     2941 gaggtccggc gcctgtcgat tcaggagagc ctaccctagg cccgaaccct gcgtcctgcg
     3001 acggagaaaa gcctaccgca cacctaccgg ctggtggccc caccctgcat tataagccaa
     3061 cagaacgggt gacgtcacga cacgacgagg gcgcgcgctc ccaaaggtac gggtgcactg
     3121 cccaacggca ccgccataac tgccgccccc gcaacagacg acaaaccgag ttctccagtc
     3181 agtgacaaac ttcacgtcag ggtccccaga tggtgcccag cccatctcac ccgaataaga
     3241 gctttcccgc attagcgaag ggctcaagac cttgggttct tgccgcccac catgcccccc
     3301 accttgtttc aacgacctca cagcccgcct cacaagcgtc ttccattcaa gactcgggaa
     3361 cagccgccat tttgctgcgc tccccccaac ccccagttca gggcaacctt gctcgcggac
     3421 ccagactaca gcccttggcg gtctctccac acgcttccgt cccaccgagc ggcccggcgg
     3481 ccacgaaagc cccggccagc ccagcagccc gctactcacc aagtgacgat cacagcgatc
     3541 cacaaacaag aaccgcgacc caaatcccgg ctgcgacgga actagctgtg ccacacccgg
     3601 cgcgtcctta tataatcatc ggcgttcacc gccccacgga gatccctccg cagaatcgcc
     3661 gagaagggac tacttttcct cgcctgttcc gctctctgga aagaaaacca gtgccctaga
     3721 gtcacccaag tcccgtccta aaatgtcctt ctgctgatac tggggttcta aggccgagtc
     3781 ttatgagcag cgggccgctg tcctgagcgt ccgggcggaa ggatcaggac gctcctgcgc
     3841 ccttcgtctg acgtggcagc gctcgccgtg acgagggggg cgcccgcggg aggcgccaaa
     3901 acccggcgcg gaggccaggc gcgccccgat ttcgatattc ctcggatctc ggatagatcg
     3961 ggattgagga tgtaggttga acttctctcg ggtgatcccg atctcggata ttcgggattg
     4021 gagtaatacc tggcgatagg cgctgccaag caatagccaa ggcgcaaggc cggccaagag
     4081 acttgcgata gttcgttcga gaggtagcct gcaagatgga tcggacgaaa ggtttgaaga
     4141 ttttccgacg agggccttgg agagttcgga aatttaaatg caactatgga tgaacgaaat
     4201 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     4261 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     4321 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     4381 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     4441 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     4501 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     4561 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     4621 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     4681 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     4741 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     4801 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     4861 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     4921 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     4981 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     5041 ttttgctggc cttttgctca catgatttaa atgaaactcg taatcggaga acgggtgcaa
     5101 tagcaaggac cagatcgaac ttgactgacg caattctgag cgtagtcgaa acccagacaa
     5161 gtcgatctcg ccaagtagct gacttgactg gttaattaag tttcgatagc ccaaggtaac
     5221 caatgagatt agggagggaa gaaagtttga tgaactactt ctgaccccta gtggtgtcca
     5281 gaaaagacca ttaaaggaat gtcttgatcg aaatcgagag atcgcctgca ggatagctga
     5341 cgaccttaag gataaatttc tggtaaggag gacacgtatg gaagtgggca agttggggaa
     5401 gccgtatccg ttgctgaatc tggcatatgt gggagtataa gacgcgcagc gtcgcatcag
     5461 gcattttttt ctgcgccaat gccggtagat agcaatccgg cccgaggggc acaaaaaacc
     5521 cctcaagacc cgtttagagg ccccaagggg ttatgctacg ataccgatag agatgggcca
     5581 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     5641 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     5701 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     5761 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtgagctg aaggtacgct
     5821 gtatctcagt cagtcaagct agcagaggcc tgcggatcct tagtcaatct ccattggctg
     5881 ttcctcatcc agttgggaat atttattctc agttaaagac ttactaaggt tttttatgat
     5941 gagattaatg atgtagtcca ggttccaaat gctaaccttg aaggttcgca tgataattag
     6001 cagtatttct gcgatcgtga cctgaaaatc caccaagtgg aacatggtgg cggccgctgg
     6061 cgggtgtgtc gagtggatgg taggatcgac aaagatctga tctgacggtt cactaaacca
     6121 gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg
     6181 gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg
     6241 acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg
     6301 atgtactgcc aaaaccgcat cagcatggta atagcgatga ctaatacgta gatgtactgc
     6361 caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg
     6421 tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg
     6481 gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact
     6541 atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc
     6601 gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac
     6661 cccgtaattg attactagat ctggctacac tcgatcagca gttagataat aaaatcgcta
     6721 tcc
//
