LOCUS       sequence-300488-        6723 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for SARS-CoV-2 ORF6 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6723)
  TITLE     SARS-CoV-2 protein Mammalian expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 6723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4252991
FEATURES             Location/Qualifiers
     source          1..6723
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(69..657)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(149..168)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1157..2366
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     2203..2222
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             2370..3398
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     polyA_signal    3425..3473
                     /note="synthetic polyadenylation signal"
     CDS             join(3739..3807,3808..4599)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3957..3976)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      4612..4748
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    4900..4971
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(4976..5022)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    5040..5088
                     /note="synthetic polyadenylation signal"
     enhancer        5169..5472
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5474..5676
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5627..5647
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    6002..6123
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6013..6032)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6067..6086
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      6238..6284
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      6314..6450
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    6495..6566
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
ORIGIN
        1 atttaaatca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
       61 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
      121 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
      181 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
      241 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
      301 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
      361 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
      421 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
      481 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
      541 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
      601 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
      661 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
      721 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
      781 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
      841 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcatttaaa
      901 tttccgaact ctccaaggcc ctcgtcggaa aatcttcaaa cctttcgtcc gatccatctt
      961 gcaggctacc tctcgaacga actatcgcaa gtctcttggc cggccttgcg ccttggctat
     1021 tgcttggcag cgcctatcgc caggtattac tccaatcccg aatatccgag atcgggatca
     1081 cccgagagaa gttcaaccta catcctcaat cccgatctat ccgagatccg aggaatatcg
     1141 aaatcggggc gcgcctggcc tccgcgccgg gttttggcgc ctcccgcggg cgcccccctc
     1201 gtcacggcga gcgctgccac gtcagacgaa gggcgcagga gcgtcctgat ccttccgccc
     1261 ggacgctcag gacagcggcc cgctgctcat aagactcggc cttagaaccc cagtatcagc
     1321 agaaggacat tttaggacgg gacttgggtg actctagggc actggttttc tttccagaga
     1381 gcggaacagg cgaggaaaag tagtcccttc tcggcgattc tgcggaggga tctccgtggg
     1441 gcggtgaacg ccgatgatta tataaggacg cgccgggtgt ggcacagcta gttccgtcgc
     1501 agccgggatt tgggtcgcgg ttcttgtttg tggatcgctg tgatcgtcac ttggtgagta
     1561 gcgggctgct gggctggccg gggctttcgt ggccgccggg ccgctcggtg ggacggaagc
     1621 gtgtggagag accgccaagg gctgtagtct gggtccgcga gcaaggttgc cctgaactgg
     1681 gggttggggg gagcgcagca aaatggcggc tgttcccgag tcttgaatgg aagacgcttg
     1741 tgaggcgggc tgtgaggtcg ttgaaacaag gtggggggca tggtgggcgg caagaaccca
     1801 aggtcttgag cccttcgcta atgcgggaaa gctcttattc gggtgagatg ggctgggcac
     1861 catctgggga ccctgacgtg aagtttgtca ctgactggag aactcggttt gtcgtctgtt
     1921 gcgggggcgg cagttatggc ggtgccgttg ggcagtgcac ccgtaccttt gggagcgcgc
     1981 gccctcgtcg tgtcgtgacg tcacccgttc tgttggctta taatgcaggg tggggccacc
     2041 agccggtagg tgtgcggtag gcttttctcc gtcgcaggac gcagggttcg ggcctagggt
     2101 aggctctcct gaatcgacag gcgccggacc tctggtgagg ggagggataa gtgaggcgtc
     2161 agtttctttg gtcggtttta tgtacctatc ttcttaagta gctgaagctc cggttttgaa
     2221 ctatgcgctc ggggttggcg agtgtgtttt gtgaagtttt ttaggcacct tttgaaatgt
     2281 aatcatttgg gtcaatatgt aattttcagt gttagacttg taaattgtcc gctaaattct
     2341 ggccgttttt ggcttttttg ttagacaaca tgggtaaaaa gcctgaactc accgcgacgt
     2401 ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg
     2461 agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg
     2521 taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatccg
     2581 ccgcgctccc gattccggaa gtgcttgaca ttggggagtt cagcgagagc ctgacctatt
     2641 gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg
     2701 ctgttctgca gccggtcgcg gaggcaatgg atgccatcgc tgccgccgat cttagccaga
     2761 cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt
     2821 tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg
     2881 tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg
     2941 aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc
     3001 gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg
     3061 ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg
     3121 agcggaggca tccggagctt gcaggatcgc cccggctccg ggcgtatatg ctccgcattg
     3181 gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc
     3241 agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg
     3301 cccgcagaag cgccgccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa
     3361 accgacgccc cagcactcgt ccgagggcaa aggaataagc tagtatgtaa gcctagtctt
     3421 agataataaa atcgctatcc atcgaagatg gatgtgtgtt ggttttttgt gtgtgtaacg
     3481 ctaggcgcgc ctggtgtacc gagaacgatc ctctcagtgc gagtctcgac gatccatatc
     3541 gttgcttggc agtcagccag tcggaatcca gcttgggacc caggaagtcc aatcgtcaga
     3601 tattgtactc aagcctggtc acggcagcgt accgatctgt ttaaacctag atattgatag
     3661 tctgatcggt caacgtataa tcgagtccta gcttttgcaa acatctatca agagacagga
     3721 tcagcaggag gctttcgcat gagtattcaa catttccgtg tcgcccttat tccctttttt
     3781 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     3841 gaagatcagt tgggtgcgcg agtgggttac atcgaactgg atctcaacag cggtaagatc
     3901 cttgagagtt ttcgccccga agaacgcttt ccaatgatga gcacttttaa agttctgcta
     3961 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     4021 tattctcaga atgacttggt tgagtattca ccagtcacag aaaagcatct tacggatggc
     4081 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     4141 ttacttctga caacgattgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     4201 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     4261 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacct tgcgtaaact attaactggc
     4321 gaactactta ctctagcttc ccggcaacag ttgatagact ggatggaggc ggataaagtt
     4381 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     4441 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     4501 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     4561 atcgctgaga taggtgcctc actgattaag cattggtaac cgattctagg tgcattggcg
     4621 cagaaaaaaa tgcctgatgc gacgctgcgc gtcttatact cccacatatg ccagattcag
     4681 caacggatac ggcttcccca acttgcccac ttccatacgt gtcctcctta ccagaaattt
     4741 atccttaaga tcccgaatcg tttaaactcg actctggctc tatcgaatct ccgtcgtttc
     4801 gagcttacgc gaacagccgt ggcgctcatt tgctcgtcgg gcatcgaatc tcgtcagcta
     4861 tcgtcagctt acctttttgg cagcgatcgc ggctcccgac atcttggacc attagctcca
     4921 caggtatctt cttccctcta gtggtcataa cagcagcttc agctacctct caattcaaaa
     4981 aacccctcaa gacccgttta gaggccccaa ggggttatgc tatcaatcgt tgcgttacac
     5041 acacaaaaaa ccaacacaca tccatcttcg atggatagcg attttattat ctaactgctg
     5101 atcgagtgta gccagatcta gtaatcaatt acggggtcat tagttcatag cccatatatg
     5161 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     5221 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     5281 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     5341 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     5401 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     5461 gctattacca tgctgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     5521 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     5581 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     5641 aggcgtgtac ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatcaga
     5701 tctttgtcga tcctaccatc cactcgacac acccgccagc ggccgccacc atgtttcatc
     5761 tcgttgactt tcaggttact atagcagaga tattactaat tattatgagg acttttaaag
     5821 tttccatttg gaatcttgat tacatcataa acctcataat taaaaattta tctaagtcac
     5881 taactgagaa taaatattct caattagatg aagagcaacc aatggagatt gattaaggat
     5941 ccgcaggcct ctgctagctt gactgactga gatacagcgt accttcagct cacagacatg
     6001 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     6061 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     6121 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
     6181 ttttaaagca agtaaaacct ctacaaatgt ggtattggcc catctctatc ggtatcgtag
     6241 cataacccct tggggcctct aaacgggtct tgaggggttt tttgtgcccc tcgggccgga
     6301 ttgctatcta ccggcattgg cgcagaaaaa aatgcctgat gcgacgctgc gcgtcttata
     6361 ctcccacata tgccagattc agcaacggat acggcttccc caacttgccc acttccatac
     6421 gtgtcctcct taccagaaat ttatccttaa ggtcgtcagc tatcctgcag gcgatctctc
     6481 gatttcgatc aagacattcc tttaatggtc ttttctggac accactaggg gtcagaagta
     6541 gttcatcaaa ctttcttccc tccctaatct cattggttac cttgggctat cgaaacttaa
     6601 ttaaccagtc aagtcagcta cttggcgaga tcgacttgtc tgggtttcga ctacgctcag
     6661 aattgcgtca gtcaagttcg atctggtcct tgctattgca cccgttctcc gattacgagt
     6721 ttc
//
