LOCUS       sequence-300489-        4371 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for SARS-CoV-2 ORF6 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4371)
  TITLE     SARS-CoV-2 protein Mammalian expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 4371)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4136065
FEATURES             Location/Qualifiers
     source          1..4371
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     47..66
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      218..264
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      294..430
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    475..546
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     rep_origin      complement(772..1360)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(852..871)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(2106..2174,2175..2966)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2324..2343)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      2979..3115
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    3267..3338
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(3343..3389)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    3407..3455
                     /note="synthetic polyadenylation signal"
     enhancer        3536..3839
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3841..4043
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3994..4014
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    join(4353..4371,1..103)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(join(4364..4371,1..12))
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
       61 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
      121 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
      181 ctacaaatgt ggtattggcc catctctatc ggtatcgtag cataacccct tggggcctct
      241 aaacgggtct tgaggggttt tttgtgcccc tcgggccgga ttgctatcta ccggcattgg
      301 cgcagaaaaa aatgcctgat gcgacgctgc gcgtcttata ctcccacata tgccagattc
      361 agcaacggat acggcttccc caacttgccc acttccatac gtgtcctcct taccagaaat
      421 ttatccttaa ggtcgtcagc tatcctgcag gcgatctctc gatttcgatc aagacattcc
      481 tttaatggtc ttttctggac accactaggg gtcagaagta gttcatcaaa ctttcttccc
      541 tccctaatct cattggttac cttgggctat cgaaacttaa ttaaccagtc aagtcagcta
      601 cttggcgaga tcgacttgtc tgggtttcga ctacgctcag aattgcgtca gtcaagttcg
      661 atctggtcct tgctattgca cccgttctcc gattacgagt ttcatttaaa tcatgtgagc
      721 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
      781 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
      841 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
      901 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
      961 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     1021 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     1081 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     1141 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     1201 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     1261 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     1321 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     1381 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     1441 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     1501 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     1561 ctcagcgatc tgtctatttc gttcatccat agttgcattt aaatttccga actctccaag
     1621 gccctcgtcg gaaaatcttc aaacctttcg tccgatccat cttgcaggct acctctcgaa
     1681 cgaactatcg caagtctctt ggccggcctt gcgccttggc tattgcttgg cagcgcctat
     1741 cgccaggtat tactccaatc ccgaatatcc gagatcggga tcacccgaga gaagttcaac
     1801 ctacatcctc aatcccgatc tatccgagat ccgaggaata tcgaaatcgg ggcgcgcctg
     1861 gtgtaccgag aacgatcctc tcagtgcgag tctcgacgat ccatatcgtt gcttggcagt
     1921 cagccagtcg gaatccagct tgggacccag gaagtccaat cgtcagatat tgtactcaag
     1981 cctggtcacg gcagcgtacc gatctgttta aacctagata ttgatagtct gatcggtcaa
     2041 cgtataatcg agtcctagct tttgcaaaca tctatcaaga gacaggatca gcaggaggct
     2101 ttcgcatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     2161 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     2221 gtgcgcgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     2281 gccccgaaga acgctttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     2341 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     2401 acttggttga gtattcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     2461 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     2521 cgattggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     2581 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     2641 cgatgcctgt agcaatggca acaaccttgc gtaaactatt aactggcgaa ctacttactc
     2701 tagcttcccg gcaacagttg atagactgga tggaggcgga taaagttgca ggaccacttc
     2761 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     2821 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     2881 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     2941 gtgcctcact gattaagcat tggtaaccga ttctaggtgc attggcgcag aaaaaaatgc
     3001 ctgatgcgac gctgcgcgtc ttatactccc acatatgcca gattcagcaa cggatacggc
     3061 ttccccaact tgcccacttc catacgtgtc ctccttacca gaaatttatc cttaagatcc
     3121 cgaatcgttt aaactcgact ctggctctat cgaatctccg tcgtttcgag cttacgcgaa
     3181 cagccgtggc gctcatttgc tcgtcgggca tcgaatctcg tcagctatcg tcagcttacc
     3241 tttttggcag cgatcgcggc tcccgacatc ttggaccatt agctccacag gtatcttctt
     3301 ccctctagtg gtcataacag cagcttcagc tacctctcaa ttcaaaaaac ccctcaagac
     3361 ccgtttagag gccccaaggg gttatgctat caatcgttgc gttacacaca caaaaaacca
     3421 acacacatcc atcttcgatg gatagcgatt ttattatcta actgctgatc gagtgtagcc
     3481 agatctagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     3541 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     3601 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     3661 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     3721 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     3781 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgc
     3841 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
     3901 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     3961 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
     4021 gggaggtcta tataagcaga gctggtttag tgaaccgtca gatcagatct ttgtcgatcc
     4081 taccatccac tcgacacacc cgccagcggc cgccaccatg tttcatctcg ttgactttca
     4141 ggttactata gcagagatat tactaattat tatgaggact tttaaagttt ccatttggaa
     4201 tcttgattac atcataaacc tcataattaa aaatttatct aagtcactaa ctgagaataa
     4261 atattctcaa ttagatgaag agcaaccaat ggagattgat taagctagct tgactgactg
     4321 agatacagcg taccttcagc tcacagacat gataagatac attgatgagt t
//
