LOCUS       sequence-300586-       13718 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human SMCHD1 Exon1-10A.12D-48 with a C-terminal 3xFLAG 
            tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13718)
  AUTHORS   Hiramuki Y, Tapscott SJ
  TITLE     Identification of SMCHD1 domains for nuclear localization, 
            homo-dimerization, and protein cleavage.
  JOURNAL   Skelet Muscle. 2018 Aug 2;8(1):24. doi: 10.1186/s13395-018-0172-z.
  PUBMED    30071896
REFERENCE   2  (bases 1 to 13718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Skelet 
            Muscle."; date: "2018-08-2"; volume: "8(1):24. doi"; pages: " 
            10.1186/s13395-018-0172-z"
COMMENT     Sequence Label: pLenti-CMV-SMCHD1-Exon1-10A.12D-48-3xFLAG
FEATURES             Location/Qualifiers
     source          1..13718
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    19..135
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        158..461
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        462..665
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     616..636
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     662..686
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             6520..6585
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    6595..7183
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6648..6668)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7066..7077)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7197..7696
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7232..7255)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7629..7648
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7717..8316
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7717..7736)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8213..8233
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             8454..8687
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8759..8880
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8796..8815)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8850..8869
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8920..9055
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9075..9094)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9076..9094)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9104..9121)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9104..9120)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9113..9135)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9262..9717
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9349..9368)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9559..9580
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9743..9847
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9848..9916,9917..10708)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10066..10085)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10879..11467
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11368..11387
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11621..11638
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11755..11776
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11791..11796,11797..11814,11815..11821)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11829..11845
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11834..11856
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11853..11869
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11853..11869
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11888..11908
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11890..11908
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        11936..12162
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             12163..12343
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    12390..12515
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    13008..13241
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             13426..13470
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tcgacggtat cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
       61 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
      121 aaaattcaaa attttatcga taagcttggg agttccgcgt tacataactt acggtaaatg
      181 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      241 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      301 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      361 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      421 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      481 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      541 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      601 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      661 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
      721 atagaagaca ccgactctag aggatccatg gcagcggcgg acggcggcgg gcctggtggg
      781 gcctctgtgg ggactgagga ggatggcgga ggcgtcggcc acaggacggt gtacttgttt
      841 gatcggcgcg aaaaggagtc cgagctcggg gaccggcctc tgcaggtcgg ggagcgctcg
      901 gactacgcgg gatttcgcgc gtgtgtgtgt cagacacttg gcatttcacc tgaagaaaaa
      961 tttgttatta caacaacaag taggaaagaa attacctgtg ataattttga tgaaactgtt
     1021 aaagatggag tcaccttata cctgctacag tcggtcaatc agttactact gacagctacg
     1081 aaagaacgaa ttgacttctt acctcactat gacacactgg ttaaaagtgg catgtatgaa
     1141 tattatgcca gtgaaggaca aaatcctttg ccatttgctc ttgcggaatt aattgacaat
     1201 tcattgtctg ctacttctcg taacattggg gttagaagaa tacagatcaa attgcttttt
     1261 gatgaaacac aaggaaaacc tgctgttgca gtgatagata atggaagagg aatgacctct
     1321 aaacagctta acaactgggc cgtgtatagg ttgtcaaaat tcacaaggca aggtgacttt
     1381 gaaagtgatc attcaggata tgttcgtcca gtaccagtgc cacgcagttt aaatagtgat
     1441 atttcctatt ttggtgttgg gggcaagcaa gctgtcttct ttgttggaca atcagccaga
     1501 atgataagca aacctgcaga ttcccaagat gttcacgagc ttgtgctttc taaagaagat
     1561 tttgagaaga aggagaaaaa taaagaggca atatatagtg gatatattag aaacagaaag
     1621 ccctctgatt ctgttcacat tacaaatgat gatgaaagat ttctacatca tcttatcata
     1681 gaggagaagg aaaaagatag ctttactgct gtggttatca caggggtaca accagaacac
     1741 atacagtact tgaaaaatta tttccacctt tggacacgac agttagcgca tatttatcac
     1801 tactatattc atggcccaaa aggaaatgaa atacgaacat caaaagaagt tgaacctttc
     1861 aacaatattg atattgaaat ttctatgttt gaaaaaggga aggtacctaa gattgtcaac
     1921 ctaagggaaa tacaagacga catgcagacg ttgtatgtaa acacagcagc tgactggtgt
     1981 actcctccta agaagagagg gcttgcacca attgaatgct acaataggat atctggtgca
     2041 ttattcacta atgacaaatt ccaggtcagc acaaataaat tgacgtttat ggatcttgag
     2101 ctaaaattga aagataagaa cacccttttt acaaggattt taaatggaca ggaacagcga
     2161 atgaaaattg acagagaatt tgctttgtgg ctgaaggact gtcatgagaa gtatgataaa
     2221 caaataaaat ttacactttt taagggagta attacacgtc ctgatcttcc ttctaaaaag
     2281 caaggtccct gggcaacata tgcagcaata gaatgggatg gaaagatata caaagcagga
     2341 cagctggtca agacaatcaa gacacttccc ctcttttatg gaagcatagt aagatttttt
     2401 ctttatggcg atcatgatgg agaagtatat gctacaggag gagaggttca aattgcaatg
     2461 gaacctcagg cactatatga tgaagtaaga actgtgccaa ttgcaaagct ggataggaca
     2521 gttgctgaga aagctgttaa aaaatatgta gaagatgaaa tggcaaggct ccctgataga
     2581 ttgtcagtaa cttggcctga aggagatgaa ttattgccta atgaggttag gcctgctgga
     2641 acccctattg gtgcgttaag aattgaaata ctgaataaaa aaggggaagc aatgcaaaag
     2701 cttccaggaa caagccatgg agggtcaaag aaactcctgg ttgagctcaa agttatttta
     2761 cattcttcaa gtggaaataa agagattatt tcgcatatta gtcaacatgg aggaaaatgg
     2821 ccttactggt ttaaaaaaat ggaaaatatt cagaagttgg ggaattatac cttgaaatta
     2881 caagttgtgt tgaatgaaag taatgcagac acttatgcag gaagaccact accatctaaa
     2941 gcaattaagt tttctgttaa agagggtaag ccagagaaat tttcatttgg tcttctggat
     3001 cttccttttc gtgttggagt tccatttaat atccctctgg agtttcagga tgaatttggt
     3061 cataccagtc aactagtaac tgatattcag ccagttcttg aagcaagtgg tttatcttta
     3121 cattatgaag aaataaccaa aggaccaaat tgtgtaattc gaggtgttac agccaagggc
     3181 cctgtaaact cttgtcaagg caagaattat aatctgaagg ttactctgcc tggcttaaaa
     3241 gaagactcac agattttgaa aattagatta ctacctggtc accctcgtcg actgaaagtg
     3301 aaacctgatt ctgaaatttt agttatagaa aatggaacag ctttcccatt tcaggtggaa
     3361 gttttagatg aatcagacaa cataacagca caaccaaaat tgattgttca ttgtaagttt
     3421 tcaggtgctc caaaccttcc agtctatgtt gtagattgca gtagttctgg aaccagtatt
     3481 ttaacaggat ctgcaattca agttcagaat attaaaaaag accagacgct taaagcaaga
     3541 attgaaatac ctagttgtaa agatgtggca cctgtggaga agactattaa gttgcttccc
     3601 agtagccatg ttgcaagact acaaatattc agtgtagaag gacaaaaggc aattcagatc
     3661 aaacatcagg atgaggttaa ttggatagcg ggtgatatta tgcataatct tatttttcaa
     3721 atgtatgatg aaggagaaag agaaatcaat ataacatcag ctttagcaga aaaaattaaa
     3781 gttaattgga ctcctgagat taacaaagaa cacttgctac agggtctgct tcctgatgtg
     3841 caagtaccaa catctgtaaa agatatgcgc tattgccagg tttcattcca agatgatcat
     3901 gtgtctttgg aaagtgcgtt tacagtaaga ccacttcctg atgaacctaa acatttaaaa
     3961 tgtgaaatga aaggaggaaa aacagtacag atgggccaag agcttcaagg agaagtagtt
     4021 ataataatta cagatcagta cggaaatcag attcaagcat tttcaccaag ttctttatct
     4081 tctttgtcaa ttgctggggt tggacttgat agctcaaatt tgaaaacaac ctttcaggaa
     4141 aacacacaga gtataagtgt aagaggcatc aaatttattc caggtcctcc tggaaataag
     4201 gatctttgtt ttacttggcg tgagttttct gactttattc gagtgcaact aatttctgga
     4261 cctcctgcta aacttctcct tatagactgg ccagaactaa aggagtccat tccagtgatt
     4321 aatggaagag atttacagaa ccctattatt gttcaacttt gtgatcagtg ggataatcca
     4381 gcaccggtac aacatgttaa aataagtctt acaaaagcta gcaatttaaa gctcatgcct
     4441 tcaaaccaac agcataaaac agatgagaaa ggcagggcta atttgggagt attcagtgtt
     4501 tttgccccta ggggagagca tactcttcag gttaaagcca tctataacaa aagtatcata
     4561 gaaggaccta taattaagtt aatgattctt ccagacccag aaaaacccgt tcgtctcaat
     4621 gttaaatatg acaaagatgc atccttctta gcagggggtc ttttcactga ttttatgatt
     4681 agtgttattt ctgaagatga cagtatcatt aaaaacatta atccagcacg tatttccatg
     4741 aaaatgtgga agctgtctac cagtgggaac cgacccccag caaatgcaga aacatttagt
     4801 tgtaataaaa taaaagataa tgacaaagaa gatggctgct tctatttcag ggataaagta
     4861 attcctaata aagtggggac atattgtatc cagtttggtt ttatgatgga taaaacaaat
     4921 attctcaaca gtgaacaggt tatagttgaa gtcctgccta atcaacctgt gaagttagta
     4981 cctaaaatta aaccacctac accagctgtt tcaaatgttc gctcagttgc cagtaggacc
     5041 ttggtcagag atctacatct tagtatcacg gatgactacg acaaccatac tggaattgat
     5101 ttggttggca ctataatagc caccattaaa ggctctaatg aggaagatac tgatacccca
     5161 ctttttattg ggaaagttag aacacttgaa ttccccttcg tgaatggttc ggctgaaatc
     5221 atgagtctgg tgctggcaga aagtagtcct ggaagggata gtactgaata ttttattgta
     5281 tttgagcccc ggctaccact tttatcaaga accttagaac catatatcct accgttcatg
     5341 ttttacaatg atgttaagaa gcagcaacaa atggcagcac ttacaaaaga aaaggaccaa
     5401 ttatctcagt ctattgttat gtataaaagt ttatttgaag ccagccaaca gcttcttaat
     5461 gaaatgaaat gtcaagttga agaagcaaga ttaaaagagg cccaattgcg aaatgaacta
     5521 aaaatacata atattgacat tcctacaaca caacaggtgc cacacattga agcacttctg
     5581 aaaagaaagc tatcagaaca agaagaactg aagaaaaaac ctagaagatc gtgtactctt
     5641 ccaaactata ctaaaggcag tggagatgtt ttgggaaaga ttgcacatct agcacaaatt
     5701 gaagatgata gagctgcgat ggttatttct tggcatctgg caagtgacat ggactgtgta
     5761 gtcaccctaa ccactgacgc tgcacgtcgt atctatgatg aaacccaagg tcgtcagcag
     5821 gtgttgcccc ttgattctat ttacaagaag actcttccag attggaaaag atctctacct
     5881 catttccgaa atggaaaatt gtattttaaa cccattggag atccagtctt tgctcgagac
     5941 ttgttaacat ttccagataa tgtagaacat tgtgaaacag tatttggtat gctgttagga
     6001 gacaccatta ttttggataa tctggatgcg gccaatcatt atagaaaaga ggttgttaaa
     6061 attacacact gtcctacact gctgaccaga gatggagatc gaattcgaag taatggaaag
     6121 tttgggggcc ttcagaataa agctcctcca atggataaac ttcggggaat ggtatttgga
     6181 gctccagttc caaaacagtg tctgatctta ggggaacaaa tagatcttct tcagcagtat
     6241 cgttctgctg tgtgcaaact agacagtgtg aataaggatc ttaacagtca attagagtac
     6301 cttcgcactc cggatatgag gaagaaaaag caagaacttg atgaacatga gaaaaatctc
     6361 aaactaatag aggaaaaact aggtatgact cccatacgta agtgtaatga ctcattgcgt
     6421 cattcaccaa aggttgagac gacagattgt ccagttcctc ctaaaagaat gagacgagaa
     6481 gctacaagac aaaataggat tataaccaaa acagatgtag actacaaaga ccatgacggt
     6541 gattataaag atcatgacat cgattacaag gatgacgatg acaagtgagt cgacaatcaa
     6601 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     6661 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     6721 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     6781 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     6841 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     6901 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     6961 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt
     7021 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     7081 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     7141 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat tctaccgggt
     7201 aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac
     7261 ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca
     7321 accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa
     7381 gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct
     7441 cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg
     7501 cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg
     7561 gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg
     7621 aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc
     7681 atctccgggc ctttcgacct gcagcccaag cttaccatga ccgagtacaa gcccacggtg
     7741 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
     7801 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
     7861 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     7921 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     7981 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     8041 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     8101 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     8161 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     8221 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
     8281 cgcacctggt gcatgacccg caagcccggt gcctgacgcc cgccccacga cccgcagcgc
     8341 ccgaccgaaa ggagcgcacg accccatgca tctcgagggc ccggtacctt taagaccaat
     8401 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     8461 gctagctcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
     8521 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     8581 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     8641 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     8701 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
     8761 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     8821 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     8881 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
     8941 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     9001 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg
     9061 acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac
     9121 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     9181 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     9241 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     9301 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     9361 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     9421 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     9481 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     9541 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     9601 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     9661 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
     9721 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     9781 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     9841 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     9901 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     9961 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
    10021 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
    10081 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
    10141 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
    10201 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
    10261 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
    10321 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
    10381 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
    10441 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
    10501 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
    10561 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
    10621 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
    10681 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
    10741 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
    10801 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
    10861 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
    10921 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
    10981 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
    11041 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
    11101 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
    11161 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
    11221 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
    11281 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
    11341 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
    11401 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
    11461 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
    11521 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
    11581 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
    11641 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
    11701 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
    11761 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
    11821 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
    11881 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag
    11941 tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta
    12001 caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc
    12061 ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat
    12121 tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac
    12181 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
    12241 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
    12301 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
    12361 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
    12421 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
    12481 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
    12541 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
    12601 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
    12661 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
    12721 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
    12781 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
    12841 ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
    12901 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
    12961 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
    13021 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
    13081 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
    13141 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
    13201 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
    13261 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
    13321 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
    13381 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
    13441 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
    13501 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
    13561 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
    13621 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
    13681 agagacagag acagatccat tcgattagtg aacggatc
//
