LOCUS       sequence-300778-        6588 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of S CDS bearing a 24nt
            deletion from SARS-CoV-2 genomic analysis in Davidson et al. 2020.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6588)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 6588)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR223 SARS-CoV-2 S 24nt-del
FEATURES             Location/Qualifiers
     source          1..6588
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     710..726
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     710..726
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     731..750
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        731..749
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    767..866
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4665..4764)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4780..4797)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4780..4796)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4789..4811)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      4860..4946
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5038..5065
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(5224..5241)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5395..5983)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5475..5494)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6076..6588,1..279))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gtgcacaaca atggtgactt ctacagcgcg gagaatctcg ctctctccag gggaagccga
       61 agtttccaaa aggtcgttga tcaaagctcg ccgcgttgtt tcatcaagcc ttacggtcac
      121 cgtaaccagc aaatcaatat cactgtgtgg cttcaggccg ccatccactg cggagccgta
      181 caaatgtacg gccagcaacg tcggttcgag atggcgctcg atgacgccaa ctacctctga
      241 tagttgagtc gatacttcgg cgatcaccgc ttccctcata atgtttaact ttgttttagg
      301 gcgactgccc tgctgcgtaa catcgttgct gctccataac atcaaacatc gacccacggc
      361 gtaacgcgct tgctgcttgg atgcccgagg catagactgt accccaaaaa aacagtcata
      421 acaagccatg aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca aggttctgga
      481 ccagttgcgt gagcgcatac gctacttgca ttacagctta cgaaccgaac aggcttatgt
      541 ccactgggtt cgtgccttca tccgtttcca cggtgtgcgt cacccggcaa ccttgggtag
      601 cagcgaagtc gaggcatttc tgtcctggct ggtctagagg aggcatgatg atatattttt
      661 atcttgtgca atgtaacatc agagattttg agacacgggc cagagctgcc aggaaacagc
      721 tatgaccatg taatacgact cactataggg gatatcagct ggatggcaaa taatgatttt
      781 attttgactg atagtgacct gttcgttgca acaaattgat aagcaatgct ttcttataat
      841 gccaactttg tacaagaaag ttgggtatta ggtgtagtgc agcttcacac ccttcagcac
      901 aggctcggag tcgtcctcgt cgaacttgca gcaagatccg caagagcagc atcccttcag
      961 gcaagagcag caagaggtca tgcagcacag catgatggtc accatcacga tagcgatcag
     1021 accagcgatg aaacccagcc agatgtacca gggccacttg atgtactgct cgtactttcc
     1081 cagttcctgc aggtcgatca gagattcgtt caggttcttg gccacctcgt tcagtctgtc
     1141 gatttccttc tggatgttca ccacagaggc gttgattccg gagatgtctc ccaggtccac
     1201 gtctggagag gtgtggttct tgaagtactt gtccagttcc tccttgaaag agtccagttc
     1261 tggctgcagt gggtcgtaca cggtgttgtt cacgattccg atcaccacgt cgcagtttcc
     1321 agacacgaag gtgttgtcgg tggtgatgat ctgtggctcg tagaagttcc tctgggtcac
     1381 gaaccagtgg gttccgttag acacgaacac tccctccctg gggaagtgag cctttccgtc
     1441 gtggcagata gctggggcgg tggtgaagtt cttttcctgg gcgggcacgt aggtcacgtg
     1501 caggaacacc actccgtgtg gagcagactg tgggaaggac atcaggtggt atccctttcc
     1561 gcagaagtcc accctcttag actggcccag cacgcattcg gacatcttgg tggcagccag
     1621 gttggcagaa gctctgattt cagcggccct gatcagctgc tgggtcacgt aggtctgcag
     1681 agactgcagt cttccggtga tcagtctgtc gatctgcacc tcggcttcca ccttgtccag
     1741 cctagacagg atgtcgttca gcacagagga gatggctccg aagttagaag acagctgctt
     1801 caccagggtg ttcagggcct gggcgttctg gttcaccacg tcctgcagct ttcccagggc
     1861 agaagcggta gaggacaggg agtcctggat ctttccgata gcagagttga actggttagc
     1921 gatcagcttc tggttttcgt acagcacgtt ctgggtcact ccgataccgt tgaaacggta
     1981 ggccatctgc atagcgaatg ggatctgcag ggcggcgcca gctccgaagg tccaaccaga
     2041 ggtgatggtt ccagccagca gggcagaggt gtactgggcg atcatctcgt cggtcagcag
     2101 tggtggcagc acggtcagac cgttgaactt ctgggcgcag atcaggtctc tagcggcgat
     2161 gtctcccagg cagtcaccgt actgcttgat gaaaccggcg tcggccaggg tcaccttgtt
     2221 gaacagcagg tcttcgatga aagacctctt agagggctta gatgggtcgg gcaggatctg
     2281 agagaagttg aatcctccga agtccttgat aggtggggtc ttgtatatct gcttcacctg
     2341 agcgaacact tcctgggtgt tcttgtcctg ctccacagcg attccggtca gagcgcggtt
     2401 cagctgggtg cagaagctgc cgtactgcag cagcaggtta gagcattcgg tagagtctcc
     2461 gcagatgtac atggtgcagt ccacgctggt cttggtcatg ctcactggca ggatttcggt
     2521 ggtcacagag atggtgaagt tggttgggat ggcgatagag ttgttgctgt aagccacgct
     2581 gttttcagcg cccaggctca tggtgtaggc gatgatgctc tgagaggcgt tggtctgggt
     2641 ctggtaagaa gcgcagatgc cagctccgat tgggatgtcg cattcgtagg agttgttcac
     2701 gtgctcggct ccgatcaggc agccggcacg ggtctggaac acgttagatc cggtgctgta
     2761 cactctccag gttggggtca gctggtcagc gtggatggcc actggcactt cggtgcagtt
     2821 cacgtcctgg tacagcacgg ccacctggtt ggaggtgttg gttcctgggg tgatcacgct
     2881 cactcctccg aaagagcagg gggtgatgtc caggatttcc agggtctgtg ggtctctcac
     2941 agcgtcggtg gtgtcggcga tgtctctacc gaactgctgg aaaggcagga acttcttgtt
     3001 ggattcggtc agcactccgg ttccggtcag tccgttgaag ttgaagttca cgcacttgtt
     3061 cttcaccagg ttggtagact tctttggtcc gcacacggta gcaggagcgt gcagcagctc
     3121 gaaagacagc accaccactc tgtatggctg gtatcccact ccgttggttg gctggaatcc
     3181 gtaagactgc agagggaagt agcagttgaa tccttccact ccgttgcatg gggtagatcc
     3241 agcctggtag atttcggtgc tgatgtccct ttcgaaaggc ttcaggttgc tcttgcggaa
     3301 cagcctgtac aggtagttgt agttgcctcc caccttagag tccaggttgt tagagttcca
     3361 agcgatcacg caaccggtga agtcgtctgg cagcttgtag ttgtagtcgg cgatctttcc
     3421 ggtctgaccg ggagcgatct gtctcacttc gtctcctctg atcacgaagg agtcagcgta
     3481 cacgttggtg aagcacaggt cgttcagctt ggttggggac acgccgtagc acttgaaggt
     3541 ggagaaggag gcagagttgt acagcacaga gtagtcggcc acgcagttgc tgattctctt
     3601 cctgttccaa gcgtacacgc tggcgaacct ggtagcgttg aacacttctc cgaatgggca
     3661 caggttggtg atgtttggga atctcacgat agactcggtg ggctgcacgc ggaagttgct
     3721 ggtctggtag attccctttt ccacggtgaa agacttcagg gtgcacttgg tctcagacag
     3781 tgggtccaga gcgcagtcca cggcgtcggt gatggtgccg ttttcgttgt acttcagcag
     3841 gaaggtcctt ggctgcaggt atcccacgta gtaagcagcg gcgccggcgg tccatccaga
     3901 gctgctgtcg cctggggtca ggtaagacct gtgcagagcc agcagggtct ggaacctggt
     3961 gatgttgatg ccgattggca ggtccaccag gggctccaga gcagagaaac cctgtggcag
     4021 gtccctcacc aggttgattg gggtgtgctt gctgtatatc ttgaagtaac cgtcgatgtt
     4081 cttgaacacg aactctctca ggttcttgaa gtttccctgc tttccttcca ggtccatcag
     4141 gaaaggctga gacacgtact cgaaggtgca gttgttagca gagctgtaca ccctgaactc
     4201 gctttccatc caagacttgt tgttcttgtg gtagtacact cccaggaatg ggtcgttgca
     4261 gaactggaac tcgcacacct tgatcaccac gttggtagcg ttgttcacga tcagcagaga
     4321 ctgggtctta gagtccaggg tggttccgaa gatccaaccc ctgatgatgt tagacttctc
     4381 ggtagaagcg aagtacacgc cgtcgttgaa tggcagcact gggttgtcga atctcttggt
     4441 gccgttggtt ccagacacgt ggatggcgtg gaaccaggtc acgttagaga agaatggcag
     4501 gaacaggtcc tgggtagagt gcagcacgct ggatctgaac accttgtctg ggtagtacac
     4561 gcccctggtg aaggagttgg tgtaggcagg tggcagctgg gttctggtgg tcaggttcac
     4621 gcactgggag ctcaccagtg gcagcagcac caggaacacg aacatgccaa cttttttgta
     4681 caaagttggc attataaaaa agcattgctc atcaatttgt tgcaacgaac aggtcactat
     4741 cagtcaaaat aaaatcatta tttggggccc gagcttaaga ctggccgtcg ttttacaacg
     4801 tcgtgactgg gaaaacatcc atgctagcgt taacgcgaga gtagggaact gccaggcatc
     4861 aaataaaacg aaaggctcag tcggaagact gggcctttcg ttttatctgt tgtttgtcgg
     4921 tgaacgctct cctgagtagg acaaatccgc cgggagcgga tttgaacgtt gtgaagcaac
     4981 ggcccggagg gtggcgggca ggacgcccgc cataaactgc caggcatcaa actaagcaga
     5041 aggccatcct gacggatggc ctttttgcgt ttctacaaac tcttcctggc tagcggtacg
     5101 cgtattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     5161 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     5221 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     5281 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     5341 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     5401 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     5461 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     5521 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     5581 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     5641 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     5701 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     5761 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     5821 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     5881 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     5941 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     6001 ttctacgggg tctgacgctc agtggaacga cgcgtaactc acgttaaggg attttggtca
     6061 tgggtggctc gagggttatt tgccgactac cttggtgatc tcgcctttca cgtagtggac
     6121 aaattcttcc aactgatctg cgcgcgaggc caagcgatct tcttcttgtc caagataagc
     6181 ctgtctagct tcaagtatga cgggctgata ctgggccggc aggcgctcca ttgcccagtc
     6241 ggcagcgaca tccttcggcg cgattttgcc ggttactgcg ctgtaccaaa tgcgggacaa
     6301 cgtaagcact acatttcgct catcgccagc ccagtcgggc ggcgagttcc atagcgttaa
     6361 ggtttcattt agcgcctcaa atagatcctg ttcaggaacc ggatcaaaga gttcctccgc
     6421 cgctggacct accaaggcaa cgctatgttc tcttgctttt gtcagcaaga tagccagatc
     6481 aatgtcgatc gtggctggct cgaagatacc tgcaagaatg tcattgcgct gccattctcc
     6541 aaattgcagt tcgcgcttag ctggataacg ccacggaatg atgtcgtc
//
