LOCUS       sequence-301-exp        3404 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1999
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLT63
FEATURES             Location/Qualifiers
     source          1..3404
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        19..37
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     95..111
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     95..111
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(795..811)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(796..812)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(837..855)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(865..882)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(865..881)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(874..896)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1023..1478
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1110..1129)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1320..1341
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1504..1608
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1609..1677,1678..2469)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1827..1846)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2640..3228
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3129..3148
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3382..3399
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aacctggctt atcgaaatta atacgactca ctatagggag accggcagat ctgatatcat
       61 cgatgaattc gagctccacc gcggtggcgg ccgctctaga actagtggat ccaccggtgc
      121 tccactatta gaggatacgg aatgctgtgg aaaagagaga tgtcagcatg cgactgaaga
      181 atctgaatac aagaaacttg tatatgtagc tgtgcatctt gtgaatgtac tcgaacgact
      241 atctcttcct tctgcacata tcgatgattc cgatgaggaa aaagagccga aagccgacgt
      301 tcgaagactg atgatcgtgt gccatgaatt acatattcca cttctacatc atacacttga
      361 agagaggatt cctgatagta acagtgcaga gttgggactt cccaaagctg cagttttgga
      421 acaacttcta gaacttgacc tagatgtgaa tgccactgat aagaataacg acactccaat
      481 gcacattctg cttcgagcca gagagtttag gaaaagcttg gttcgagcac tgctcgtacg
      541 tggcacatgg ctgttcgcaa ggaatcggca cggagatgtg gtgttgaatg tgatgaaacg
      601 tatgaaagcg ttgaatcacg ccaactttga tgatctacca ctaggtgcgt gtcgaatcag
      661 aaattgagag ttattagatt atttggaacg cgaataactt cattatacca cggagagagc
      721 cagaaattga agaaattggt taaggagaat ggaatttaat gaataatagc gcaccaattc
      781 gatatcaagc ttatcgatac cgtcgacctc gagggggggc ccggtaccca attcgcccta
      841 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
      901 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
      961 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     1021 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     1081 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     1141 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     1201 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     1261 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     1321 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     1381 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     1441 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
     1501 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     1561 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     1621 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     1681 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     1741 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     1801 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     1861 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     1921 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     1981 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     2041 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     2101 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     2161 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     2221 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     2281 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     2341 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     2401 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     2461 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     2521 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     2581 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     2641 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     2701 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     2761 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     2821 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     2881 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     2941 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3001 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3061 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3121 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3181 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3241 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3301 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3361 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagc
//
