LOCUS       sequence-30132-e         717 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Potter CJ, Tasic B, Russler EV, Liang L, Luo L
  TITLE     The Q system: a repressible binary system for transgene expression, 
            lineage tracing, and mosaic analysis.
  JOURNAL   Cell. 2010 Apr 30. 141(3):536-48.
  PUBMED    20434990
REFERENCE   2  (bases 1 to 717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Apr 30. 141(3):536-48."
COMMENT     Sequence Label: pQF-M2ET
FEATURES             Location/Qualifiers
     source          1..717
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(196..300)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    complement(326..359)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cttggaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
       61 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
      121 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
      181 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
      241 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
      301 cacatttccc cgaaaagtgc cacctataac ttcgtatagc atacattata cgaagttatg
      361 acgtttggaa ttaattctag ttccagtgaa atccaagcat tttctaaatt aaatgtattc
      421 ttattattat agttgttatt tttgatatat ataaacaaca ctattatgcc caccattttt
      481 ttgagatgca tctacacaag gaacaaacac tggatgtcac tttcagttca aattgtaacg
      541 ctaatcactc cgaacaggtc acaaaaaatt accttaaaaa gtcataatat taaattagaa
      601 taaatatagc tgtgagggaa atatatacaa atatattgga gcaaataaat tgtacataca
      661 aatatttatt actaatttct attgagacga aatgaaccac tcggaccatt tgagcga
//
