LOCUS       sequence-30158-e         671 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Sun Q, Fan W, Chen K, Ding X, Chen S, Zhong Q
  TITLE     Identification of Barkor as a mammalian autophagy-specific factor 
            for Beclin 1 and class III phosphatidylinositol 3-kinase.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Dec 9. 105(49):19211-6.
  PUBMED    19050071
REFERENCE   2  (bases 1 to 671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2008 Dec 9. 105(49):19211-6."
COMMENT     Sequence Label: pcDNA3-Barkor 1-91
FEATURES             Location/Qualifiers
     source          1..671
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..37)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(53..82)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(477..496)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(478..496)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(541..640)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(570..590)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ctggagaccg tttaactcaa tgatgatgat gatgatggtc gacggcgcta ttcagatcct
       61 cttctgagat gagtttttgt tcgggcccaa gcttggtacc gagctcggat ccactagtcc
      121 agtgtggtgg aattctgcag atatccagca cagtggcggc cgcttcttct tgcttgctct
      181 taagtcggct taacctttcc ttcttgtcga taaacctctc ccggtcgcgg ccgtcgaagt
      241 agacgaaatc gccgctctga acgcatttgg cgcaggtcag ccgccggcgg gtagtgttgc
      301 acagcgggca gcgctccaca gccacgtaca gcccctccgc atcgtccacg gagtccacca
      361 ggtcccgggc gagcggccgg ggcccgcagc caggagcctc cagcgcccgg gctcccttcc
      421 cactgggaga cgccatagtc tagagggccc gtttaaacgc tagccagctt gggtctccct
      481 atagtgagtc gtattaattt cgataagcca gtaagcagtg ggttctctag ttagccagag
      541 agctctgctt atatagacct cccaccgtac acgcctaccg cccatttgcg tcaatggggc
      601 ggagttgtta cgacattttg gaaagtcccg ttgattttgg tgccaaaaca aactcccatt
      661 gacgtcaatg g
//
