LOCUS       sequence-30189-e         680 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Griffis ER, Stuurman N, Vale RD
  TITLE     Spindly, a novel protein essential for silencing the spindle 
            assembly checkpoint, recruits dynein to the kinetochore.
  JOURNAL   J Cell Biol. 2007 Jun 18. 177(6):1005-15.
  PUBMED    17576797
REFERENCE   2  (bases 1 to 680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2007 Jun 18. 177(6):1005-15."
COMMENT     Sequence Label: pMT-Mad2-GFP
FEATURES             Location/Qualifiers
     source          1..680
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(27..68)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(149..170)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     498..517
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atggtgaggt gatgatgacc ggtacgcgta gaatcgagac cgaggagagg gttagggata
       61 ggcttacctt cgaagggccc tctagcttac gtcacgtgga ccggtgcttg tacagctcgt
      121 ccatgccgag agtgatcccg gcggcggtca cgaactccag caggaccatg tgatcgcgct
      181 tctcgttggg gtctttgctc agggcggact gggtgctcag gtagtggttg tcgggcagca
      241 gcacggggcc gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc
      301 cgtcctcgat gttgtggcgg atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg
      361 ccatgatata gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc aggatgttgc
      421 cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt caccagggtg tcgccctcga
      481 acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct
      541 ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt
      601 agcggctgaa gcactgcacg ccgtaggtca gggtggtcac gagggtgggc cagggcacgg
      661 gcagcttgcc ggtggtgcag
//
