LOCUS       sequence-30205-e         716 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Morimoto-Tomita M, Uchimura K, Bistrup A, Lum DH, Egeblad M, 
            Boudreau N, Werb Z, Rosen SD
  TITLE     Sulf-2, a proangiogenic heparan sulfate endosulfatase, is 
            upregulated in breast cancer.
  JOURNAL   Neoplasia. 2005 Nov . 7(11):1001-10.
  PUBMED    16331886
REFERENCE   2  (bases 1 to 716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neoplasia. 
            2005 Nov . 7(11):1001-10."
COMMENT     Sequence Label: f1H ∂HD (267)
FEATURES             Location/Qualifiers
     source          1..716
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..74
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        55..73
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      91..100
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     sig_peptide     97..156
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             217..240
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac
       61 gactcactat agggagaccc aagctggcta gccaccatgg agacagacac actcctgcta
      121 tgggtactgc tgctctgggt tccaggttcc actggtgacg cggcccagcc ggccaggcgc
      181 gcgcgccgta cgaagcttgg taccgagctc ggatccgatt acaaggatga cgacgataag
      241 ccagcagcat cgactgtcag atccccgagg ttcagaggac ggatacagca ggaacgaaaa
      301 aacatccgac ccaacattat tcttgtgctt accgatgatc aagatgtgga gctggggtcc
      361 ctgcaagtca tgaacaaaac gagaaagatt atggaacatg ggggggccac cttcatcaat
      421 gcctttgtga ctacacccat gtgctgcccg tcacggtcct ccatgctcac cgggaagtat
      481 gtgcacaatc acaatgtcta caccaacaac gagaactgct cttccccctc gtggcaggcc
      541 atgcatgagc ctcggacttt tgctgtatat cttaacaaca ctggctacag aacagccttt
      601 tttggaaaat acctcaatga atataatggc agctacatcc cccctgggtg gcgagaatgg
      661 cttgnattaa tcaagaattc tcgcttctat aattacactg tttgtcgcaa tggcat
//
