LOCUS       sequence-302067-        9763 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of SbfI endonuclease in S. cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9763)
  AUTHORS   Gnugge R, Symington LS
  TITLE     Efficient DNA double-strand break formation at single or multiple 
            defined sites in the Saccharomyces cerevisiae genome.
  JOURNAL   Nucleic Acids Res. 2020 Oct 14. pii: 5923424. doi: 
            10.1093/nar/gkaa833.
  PUBMED    33053188
REFERENCE   2  (bases 1 to 9763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Oct 14. pii: 5923424. doi: 10.1093/nar/gkaa833."
COMMENT     Sequence Label: pRG706_lexO-SbfI_LexA-TF_LEU2MX
FEATURES             Location/Qualifiers
     source          1..9763
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     2597..2619
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2611..2628
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2612..2628
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2635..2654
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2635..2653
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4040..4060
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4067..4090
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      4140..4389
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             join(5059..5310,5311..5664)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     primer_bind     5602..5623
                     /label=LexA
                     /note="LexA DNA binding domain, forward primer"
     CDS             5683..6618
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             5731..6621
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     terminator      7085..7334
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     promoter        complement(7497..7515)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7536..7552)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7536..7552)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7549..7571)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7560..7576
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(7835..7852)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8006..8594)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8086..8105)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8773..9564,9565..9633))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9396..9415
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9634..9738)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacgtcgaaa gaacgcggca ttagcaacat ttgcgaagcg aacgattttg cgaactgcta
       61 ttaattaact ctggcgcgcc taactttttg tgtggtgccc tcctccttgt caatattaat
      121 gttaaagtgc aattcttttt ccttatcacg ttgagccatt agtatcaatt tgcttacctg
      181 tattccttta catcctcctt tttctccttc ttgataaatg tatgtagatt gcgtatatag
      241 tttcgtctac cctatgaaca tattccattt tgtaatttcg tgtcgcatat gatccgcagg
      301 ctaaccggaa cctgtattat ttagtttatg ctacgttaaa taaagacctt tcgttcacat
      361 aactgaatgt gtaatggcct tgagatttca agcataccaa gttggtggag acggggtcgt
      421 tacaaaagac tctttaagcc aagatttcct tgacagcctt ggcgatagca tcgccaacct
      481 cagtggtaga gttagaacca ccaaggtcac cggttctgac acctgcatcc aagacatttc
      541 taacagcttc ttcaagagcc ctaccttctt caaccaaatc caaggataac ttcaacatca
      601 tagctgcaga taagatggta gcaattgggt taaccttgtt tgctggtaaa tctggggcag
      661 aaccatgaca tggttcgtac aaaccgaatg ccttgttagt gtcaggtagg gaagctagag
      721 atgcagaagg taataaaccc aaagaacctg gaataacaga ggcttcatcg gagataatat
      781 caccaaacat gttgttggta ataacaacac cgtttagctt agttggtgat ttaaccaaaa
      841 tcatagcagc agagtcgatc aattggtgct gaacagttaa ttgtgggaac tcagtcttga
      901 tggtttcttc aacagtcttt ctccacaatc tggaagaggc aagcacgtta gccttgtcaa
      961 gagaccagat tggtaatggt gggttttgtt gcaatgccaa gaaagcagcc attcttgtaa
     1021 ttctttgaac ttcaggaaca ctgtatttct cagagtccca agcaactccg tcaccttcat
     1081 cttcttttct ttcaccaaag tagataccac caaccaattc tctaacgacg acgaaatcag
     1141 taccctttgc atattcaggc ttcaaaggag aaagatctag taaagaatca gaagcaaagt
     1201 tacatggtct caagttggcg tatagaccca attcctttct gatcttcaat agaccttgtt
     1261 ctggtctaac tgcgcccgta ccccattttg gaccaccaac agcacctagt aagacagcat
     1321 cggctttctt agaggcttca agagcttcat ctggtaaagg agtgccagtg gcatcgatgg
     1381 cagcaccccc gatcaagtga tgttggaaat tgaacttaat ttctggacgg acttcagcaa
     1441 tggcattcaa gaccttaata gcttcgtcag taacttcttt accgacgtga tcacccggta
     1501 ggacaacgat attcttagac atggtataat ctgtgtagtg tgggatactt tttacttctt
     1561 caaataggta tcaacaataa aaataaatcg aagcaaatgt aggaatgcgt taaagcagat
     1621 gtacttactt agagtacata gatatattta tataattcaa tatataaaag tatatgaagc
     1681 atctgatgtt gaacctgtca tgactctaaa ctggaatggg tagttatgct tctgaaggtt
     1741 ttccgttaga aaatgagtgc agggatcagg cccggaaccg gttttagcct gaaaggaaaa
     1801 gaaaaaaatg cggtgattac tagcacgtga ctgcgctgaa ttggaatcta ccacaagtac
     1861 agcgaatggc aagagggaag gggaatatat tgtgcctggc ggtagcgtct gctatatcaa
     1921 atgttaaaca taactaaatg cagtagtgaa tagagtaatg tatttctaca tctgtgggta
     1981 gggccaatgg gagcccgatg tctattgtac agatattttc cttttatagt taatcaattc
     2041 aacttggaaa tcattgagtc gatgtctact tggataactg gagccgtggc agcgtttgaa
     2101 atttgacgag tcttcttgtt ttgttcgcgc attttcagtt tattcaaacg ggagatggca
     2161 ccgaatccac tgacttctct gttcaatttg aatggtaata ggaagaaagt acatttcgag
     2221 aaattttcgc ccaacgtcga aatctcatgg atgatttcct cgatacaaat gacaccttca
     2281 tcacctaatt tctcttcaac gatattgttg tcattcaaga ttatctcttt tggttcttta
     2341 tcttctggcc tttgccacat tattctagtt ctcttttgga ttaaagaacg gatagatgca
     2401 agtgaaggtt gtccgataac aatatatggt gatacaagtt tcaacaatgg gtacacgtcc
     2461 ttggtaagtt tgatgaaaac accagtgttc aactcaacaa gtctcaaaag agataacact
     2521 ttgtaagcct ttgctgggat cttcacagcg atcggatgtg ctgcaaggcg attaagttgg
     2581 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga attgtaatac
     2641 gactcactat agggcgaatt ggagaaacag aataatatat aaacctgtat aatataacct
     2701 tgaagactat atttcttttc gcggccgcct atagggcgaa ttggagctcc ctaggtgctg
     2761 tatatactca cagcataact gtatatacac ccagggtcta ggtgctgtat atactcacag
     2821 cataactgta tatacaccca gggtctaggt gctgtatata ctcacagcat aactgtatat
     2881 acacccaggg tctaggtgct gtatatactc acagcataac tgtatataca cccagggtct
     2941 agagcatgtg ctctgtatgt atataaaact cttgttttct tcttttctct tctttcctta
     3001 tacattagga cctttgcagc ataaattact atacttctat actagtggat cccccgggct
     3061 gcaggaattc atgaactcct ccgatggtat tgatggtact gttgcttcta ttgatactgc
     3121 tagagctttg ttgaagagat tcggttttga tgcccaaaga tacaacgtta gatctgctgt
     3181 tactttgttg gctttggctg gtttgaaacc tggtgataga tgggttgatt ctactactcc
     3241 aagattgggt gttcaaaaga tcatggattg gtctggtgaa cattgggcta aaccatacgc
     3301 tactggttca agagaagatt tcagaaaaaa gaccttgaga caatgggttg acaatggttt
     3361 tgctgttttg aacgctgaca acttgaatat tgctaccaac tctcagttga acgagtactg
     3421 tttgtctgat gaagccttgc aagccttgag agcttatggt actgaaggtt ttgaagaatc
     3481 cttggtcgtt tttttggacg aagctagtaa agctgttaag gctagagctg aagctctaca
     3541 agctgctatg atttctgttg atttgccagg tggtgaagag tttttgttgt ctccagcagg
     3601 tcaaaaccca ctgttgaaaa aaatggtcga agagttcgtt ccaagatttg ctccaagatc
     3661 tactgtcttg tatttgggtg ataccagagg taagcactct ttgttcgaaa gagaaatctt
     3721 cgaggaagtt ttgggtttga cttttgatcc acatggtaga atgcctgatt tgatcttgca
     3781 tgatgaagtt agaggttggc tgtttttgat ggaagccgtt aagtctaaag gtccattcga
     3841 tgaagaaaga cacagatcat tgcaagagtt gttcgttact ccatctgccg gtttgatttt
     3901 cgttaactgt ttcgaaaacc gtgagtctat gagacagtgg ttgccagaat tggcttggga
     3961 aactgaagcc tgggttgctg aagatccaga tcatttgatt catttgaacg gctccagatt
     4021 tttgggtcca tacgaaagac caaagaagaa gagaaaggtc gatatcgact acaaggatga
     4081 cgacgacaag aagctttaac taactaacct cgagacaggc cccttttcct ttgtctatat
     4141 catgtaatta gttatgtcac gcttacattc acgccctcct cccacatccg ctctaaccga
     4201 aaaggaagga gttagacaac ctgaagtcta ggtccctatt tatttttttt aatagttatg
     4261 ttagtattaa gaacgttatt tatatttcaa atttttcttt tttttctgta caaacgcgtg
     4321 tacgcatgta acattatact gaaaaccttg cttgagaagg ttttgggacg ctcgaaggct
     4381 ttaatttgca aggttcgcag tttacactct catcgtcgct ctcatcatcg cttccgttgt
     4441 tgttttcctt agtagcgtct gcttggtacc cagcttcggc cgctctagag aagcgggtaa
     4501 gctgccacag caattaatgc acaacattta acctacattc ttccttatcg gatcctcaaa
     4561 acccttaaaa acatatgcct caccctaaca tattttccaa ttaaccctca atatttctct
     4621 gtcacccggc ctctattttc cattttcttc tttacccgcc acgcgttttt ttctttcaaa
     4681 tttttttctt ccttcttctt tttcttccac gtcctcttgc ataaataaat aaaccgtttt
     4741 gaaaccaaac tcgcctctct ctctcctttt tgaaatattt ttgggtttgt ttgatccttt
     4801 ccttcccaat ctctcttgtt taatatatat tcatttatat cacgctctct ttttatcttc
     4861 ctttttttcc tctctcttgt attcttcctt cccctttcta ctcaaaccaa gaagaaaaag
     4921 aaaaggtcaa tctttgttaa agaataggat cttctactac atcagctttt agatttttca
     4981 cgcttactgc ttttttcttc ccaagatcga aaatttactg aattaacaac tagtggatcc
     5041 cccgggctgc aggaattcat gaaagcgtta acggccaggc aacaagaggt gtttgatctc
     5101 atccgtgatc acatcagcca gacaggtatg ccgccgacgc gtgcggaaat cgcgcagcgt
     5161 ttggggttcc gttccccaaa cgcggctgaa gaacatctga aggcgctggc acgcaaaggc
     5221 gttattgaaa ttgtttccgg cgcatcacgc gggattcgtc tgttgcagga agaggaagaa
     5281 gggttgccgc tggtaggtcg tgtggctgcc ggtgaaccac ttctggcgca acagcatatt
     5341 gaaggtcatt atcaggtcga tccttcctta ttcaagccga atgctgattt cctgctgcgc
     5401 gtcagcggga tgtcgatgaa agacatcggc attatggatg gtgacttgct ggcagtgcat
     5461 aaaactcagg atgtacgtaa cggtcaggtc gttgtcgcac gtattgatga cgaggttacc
     5521 gttaagcgcc tgaaaaaaca gggcaataaa gtcgaactgt tgccagaaaa tagcgagttt
     5581 aaaccaattg tcgtagatct tcgtcagcag agcttcacca ttgaagggct ggcggttggg
     5641 gttattcgca acggcgactg gctgggttct aaggatatct ctgctggaga catgagagct
     5701 gccaaccttt ggccaagccc gctcatgatc aaacgctcta agaagaacag cctggccttg
     5761 tccctgacgg ccgaccagat ggtcagtgcc ttgttggatg ctgagccccc catactctat
     5821 tccgagtatg atcctaccag acccttcagt gaagcttcga tgatgggctt actgaccaac
     5881 ctggcagaca gggagctggt tcacatgatc aactgggcga agagggtgcc aggctttgtg
     5941 gatttgaccc tccatgatca ggtccacctt ctagaatgtg cctggttaga gatcctgatg
     6001 attggtctcg tctggcgctc catggagcac ccagtgaagc tactgtttgc tcctaacttg
     6061 ctcttggaca ggaaccaggg aaaatgtgta gagggcatgg tggagatctt cgacatgctg
     6121 ctggctacat catctcggtt ccgcatgatg aatctgcagg gagaggagtt tgtgtgcctc
     6181 aaatctatta ttttgcttaa ttctggagtg tacacatttc tgtccagcac cctgaagtct
     6241 ctggaagaga aggaccatat ccaccgagtc ctggacaaga tcacagacac tttgatccac
     6301 ctgatggcca aggcaggcct gaccctgcag cagcagcacc agcggctggc ccagctcctc
     6361 ctcatcctct cccacatcag gcacatgagt aacaaaggca tggagcatct gtacagcatg
     6421 aagtgcaaga acgtggtgcc cctctatgac ctgctgctgg agatgctgga cgcccaccgc
     6481 ctacatgcgc ccactagccg tggaggggca tccgtggagg agacggacca aagccacttg
     6541 gccactgcgg gctctacttc atcgcattcc ttgcaaaagt attacatcac gggggaggca
     6601 gagggtttcc ctgccacagt cgatatcatg caggatctgc cgggcaacga taacagcacc
     6661 gcgggcgaat ttccaggtat tactttgaga atccaagaaa ctgatatgtt gtataaaggt
     6721 gatactttgt atttggattg gttggaagat ggtattgctg aattggtttt cgatgctcca
     6781 ggttctgtta ataaattgga tactgctgtt gcttctttgg gtgaagctat tggtgttttg
     6841 gaacaacaat ctgatttgat ttgggaaact ttgactgtta aagatgctaa agttaatttc
     6901 gattctggtt tggaaaaatt cgaagaagct attccatctg ctgatgattt cgatccagtt
     6961 gctgaaagaa gatcctctgg tgaatttaga gctgaaagac attctggtgg tactgatttg
     7021 tgtttcaagc tttaaacgcg tttaaagctt atcctcgaga caggcccctt ttcctttgtc
     7081 tatatcatgt aattagttat gtcacgctta cattcacgcc ctcctcccac atccgctcta
     7141 accgaaaagg aaggagttag acaacctgaa gtctaggtcc ctatttattt tttttaatag
     7201 ttatgttagt attaagaacg ttatttatat ttcaaatttt tctttttttt ctgtacaaac
     7261 gcgtgtacgc atgtaacatt atactgaaaa ccttgcttga gaaggttttg ggacgctcga
     7321 aggctttaat ttgcaaggtt cgcagtttac actctcatcg tcgctctcat catcgcttcc
     7381 gttgttgttt tccttagtag cgtctgcttg gtacccagct tcggccgtat ttacagataa
     7441 cgatgtcgtt attatatata tatatatata ttgtcacttg tacccagctt ttgttccctt
     7501 tagtgagggt taatttcgag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     7561 tgttatccgc tcacaacagc tgtaatatat agtagtaacc tgaaaaatag tcaatgcccg
     7621 tggttcattt gccaatagaa cagtcgatgt gatcaatgta cacgaaagtc aatataaaat
     7681 atccaaatag ccaatggtgt caggacggct aaggcaaatg caactttgaa atacttattc
     7741 ggtttgcatc ttggcgcgcc tcttcctgca ggcattaatg aatcggccaa cgcgcgggga
     7801 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     7861 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     7921 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     7981 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     8041 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     8101 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     8161 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     8221 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     8281 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     8341 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     8401 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     8461 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     8521 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     8581 aaaaaggatc tcaagaagat cctttgatct tttgtttaaa cctacggggt ctgacgctca
     8641 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     8701 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     8761 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     8821 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     8881 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     8941 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     9001 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     9061 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     9121 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     9181 gtgcaaaaaa gcggttagct ccttcggtcc tccgatggtt gtcagaagta agttggccgc
     9241 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     9301 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     9361 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     9421 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     9481 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     9541 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     9601 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     9661 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     9721 acaaataggg gttccgcgca catttccccg aaaagtgcca cct
//
