LOCUS       sequence-302069-        6892 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6892)
  AUTHORS   Nam HS, Benezra R
  TITLE     High levels of Id1 expression define B1 type adult neural stem 
            cells.
  JOURNAL   Cell Stem Cell. 2009 Nov 6. 5(5):515-26.
  PUBMED    19896442
REFERENCE   2  (bases 1 to 6892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2009 Nov 6. 5(5):515-26."
COMMENT     Sequence Label: pGFAP::MyrTomato
FEATURES             Location/Qualifiers
     source          1..6892
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    115..136
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(151..156,157..174,175..181)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    189..205
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     194..216
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     213..229
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     213..229
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2506..3936
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     primer_bind     complement(4505..4522)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4505..4521)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4514..4536)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4730..4749)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4849..4871
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4909..4927)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4995..5099
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5100..5168,5169..5960)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6131..6719
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6620..6639
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6873..6890
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
       61 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
      121 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      181 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa
      241 ttcgagctcg gtaccagatc tgagctccca cctccctctc tgtgctggga ctcacagagg
      301 gagacctcag gaggcagtct gtccatcaca tgtccaaatg cagagcatac cctgggctgg
      361 gcgcagtggc gcacaactgt aattccagca ctttgggagg ctgatgtgga aggatcactt
      421 gagcccagaa gttctagacc agcctgggca acatggcaag accctatctc tacaaaaaaa
      481 gttaaaaaat cagccacgtg tggtgacaca cacctgtagt cccagctatt caggaggctg
      541 aggtgagggg atcacttaag gctgggaggt tgaggctgca gtgagtcgtg gttgcgccac
      601 tgcactccag cctgggcaac agtgagaccc tgtctcaaaa gacaaaaaaa aaaaaaaaaa
      661 aaaaaagaac atatcctggt gtggagtagg ggacgctgct ctgacagagg ctcgggggcc
      721 tgagctggct ctgtgagctg gggaggaggc agacagccag gccttgtctg caagcagacc
      781 tggcagcatt gggctggccg ccccccaggg cctcctcttc atgcccagtg aatgactcac
      841 cttggcacag acacaatgtt cggggtgggc acagtgcctg cttcccgccg caccccagcc
      901 cccctcaaat gccttccgag aagcccattg agcagggggc ttgcattgca ccccagcctg
      961 acagcctggc atcttgggat aaaagcagca cagcccccta ggggctgccc ttgctgtgtg
     1021 gcgccaccgg cggtggagaa caaggctcta ttcagcctgt gcccaggaaa ggggatcagg
     1081 ggatgcccag gcatggacag tgggtggcag ggggggagag gagggctgtc tgcttcccag
     1141 aagtccaagg acacaaatgg gtgaggggac tgggcagggt tctgaccctg tgggaccaga
     1201 gtggagggcg tagatggacc tgaagtctcc agggacaaca gggcccaggt ctcaggctcc
     1261 tagttgggcc cagtggctcc agcgtttcca aacccatcca tccccagagg ttcttcccat
     1321 ctctccaggc tgatgtgtgg gaactcgagg aaataaatct ccagtgggag acggaggggt
     1381 ggccagggaa acggggcgct gcaggaataa agacgagcca gcacagccag ctcatgtgta
     1441 acggctttgt ggagctgtca aggcctggtc tctgggagag aggcacaggg aggccagaca
     1501 aggaaggggt gacctggagg gacagatcca ggggctaaag tcctgataag gcaagagagt
     1561 gccggccccc tcttgcccta tcaggacctc cactgccaca tagaggccat gattgaccct
     1621 tagacaaagg gctggtgtcc aatcccagcc cccagcccca gaactccagg gaatgaatgg
     1681 gcagagagca ggaatgtggg acatctgtgt tcaagggaag gactccagga gtctgctggg
     1741 aatgaggcct agtaggaaat gaggtggccc ttgagggtac agaacaggtt cattcttcgc
     1801 caaattccca gcaccttgca ggcacttaca gctgagtgag ataatgcctg ggttatgaaa
     1861 tcaaaaagtt ggaaagcagg tcagaggtca tctggtacag cccttccttc cctttttttt
     1921 tttttttttt gtgagacaag gtctctctct gttgcccagg ctggagtggc gcaaacacag
     1981 ctcactgcag cctcaaccta ctgggctcaa gcaatcctcc agcctcagcc tcccaaagtg
     2041 ctgggattac aagcatgagc caccccactc agccctttcc ttccttttta attgatgcat
     2101 aataattgta agtattcatc atggtccaac caaccctttc ttgacccacc ttcctagaga
     2161 gagggtcctc ttgcttcagc ggtcagggcc ccagacccat ggtctggctc caggtaccac
     2221 ctgcctcatg caggagttgg cgtgcccagg aagctctgcc tctgggcaca gtgacctcag
     2281 tggggtgagg ggagctctcc ccatagctgg gctgcggccc aaccccaccc cctcaggcta
     2341 tgccaggggg tgttgccagg ggcacccggg catcgccagt ctagcccact ccttcataaa
     2401 gccctcgcat cccaggagcg agcagagcca gagcaggttg gagaggagac gcatcacctc
     2461 cgctgctcgc ggggatccac catgggttgc tgtttctcca agaccatggt gagcaaggga
     2521 gaggaggtca tcaaagagtt catgcgcttc aaggtgcgca tggagggctc catgaacggc
     2581 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
     2641 aagctgaagg tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag
     2701 ttcatgtacg gctccaaggc gtacgtgaag caccccgccg acatccccga ttacaagaag
     2761 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggtctg
     2821 gtgaccgtga cccaggactc ctccctgcag gacggcacgc tgatctacaa ggtgaagatg
     2881 cgcggcacca acttcccccc cgacggcccc gtaatgcaga agaagaccat gggctgggag
     2941 gcctccaccg agcgcctgta cccccgcgac ggcgtgctga agggcgagat ccaccaggcc
     3001 ctgaagctga aggacggcgg ccactacctg gtggagttca agaccatcta catggccaag
     3061 aagcccgtgc aactgcccgg ctactactac gtggacacca agctggacat cacctcccac
     3121 aacgaggact acaccatcgt ggaacagtac gagcgctccg agggccgcca ccacctgttc
     3181 ctggggcatg gcaccggcag caccggcagc ggcagctccg gcaccgcctc ctccgaggac
     3241 aacaacatgg ccgtcatcaa agagttcatg cgcttcaagg tgcgcatgga gggctccatg
     3301 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
     3361 accgccaagc tgaaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc
     3421 ccccagttca tgtacggctc caaggcgtac gtgaagcacc ccgccgacat ccccgattac
     3481 aagaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     3541 ggtctggtga ccgtgaccca ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg
     3601 aagatgcgcg gcaccaactt cccccccgac ggccccgtaa tgcagaagaa gaccatgggc
     3661 tgggaggcct ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg cgagatccac
     3721 caggccctga agctgaagga cggcggccgc tacctggtgg agttcaagac catctacatg
     3781 gccaagaagc ccgtgcaact gcccggctac tactacgtgg acaccaagct ggacatcacc
     3841 tcccacaacg aggactacac catcgtggaa cagtacgagc gctccgaggg ccgccaccac
     3901 ctgttcctgt acggcatgga cgagctgtac aagtaaggat ccgcagatcc cggccagata
     3961 ccgatgctgc cgcagcaaaa gcaggagcag atgccgccgt cgcaggcgaa gatgtcgcag
     4021 acggaggagg cgatgctgcc ggcggaggag gcgaagtaag tagagggctg ggctgggctg
     4081 tggggggtgt ggggtgcggg actgggcagt ctgggagtcc ctctcaccac ttttcttacc
     4141 tttctaggat gctgccgtcg ccgccgctca tacaccataa ggtgtaaaaa atactagatg
     4201 cacagaatag caagtccatc aaaactcctg cgtgagaatt ttaccagact tcaagagcat
     4261 ctcgccacat cttgaaaaat gccaccgtcc gatgaaaaac aggagcctgc taaggaacaa
     4321 tgccacctgt caataaatgt tgaaaactca tcccattcct gcctcttggt ccttgggctt
     4381 ggggaggggt gcgcggatgt ggttagggaa catgactggt caaatgggaa gggcttcaaa
     4441 agaattccca atattgacta ccaagccacc tgtacagatc tgcctgcagg catgcaagct
     4501 tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     4561 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     4621 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc
     4681 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct
     4741 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     4801 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     4861 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     4921 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     4981 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     5041 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     5101 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     5161 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     5221 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     5281 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     5341 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     5401 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     5461 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     5521 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     5581 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     5641 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     5701 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     5761 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     5821 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     5881 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     5941 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     6001 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     6061 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     6121 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     6181 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     6241 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     6301 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     6361 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     6421 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     6481 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     6541 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     6601 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     6661 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     6721 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     6781 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     6841 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag ga
//
