LOCUS       sequence-30246-e         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Conklin BR, Farfel Z, Lustig KD, Julius D, Bourne HR
  TITLE     Substitution of three amino acids switches receptor specificity of 
            Gq alpha to that of Gi alpha.
  JOURNAL   Nature. 1993 May 20. 363(6426):274-6.
  PUBMED    8387644
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            1993 May 20. 363(6426):274-6."
COMMENT     Sequence Label: qo5
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(240..260)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          390..455
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        539..557
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     539..556
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat
       61 ggctgattat gatctctagt caaggcacta tacatcaaat atttttccat aattttcttg
      121 tatagcagtg cagctttttc ctttgtggtg taaatagcaa agcaagcaag agttctatta
      181 ctaaacacag catgactcaa aaaacttagc aattctgaag gaaagtcctt ggggtcttct
      241 acctttctct tcttttttgg aggagtagaa tgttgagagt cagcagtagc ctcatcatca
      301 ctagatggca tttcttctga gcaaaacagg ttttcctcat taaaggcatt ccaccactgc
      361 tcccattcat cagttccata ggttggaatc taaaatacac aaacaattag aatcagtagt
      421 ttaacacatt atacacttaa aaattttata tttaccttag agctttaaat ctctgtaggt
      481 agtttgtcca attatgtcac accacagaag taaggttcct tcacaaagat cctctagcat
      541 ttaggtgaca ctatagaata gggccctcta gatgcattag tacaagccac agcccttcag
      601 gttcagctgc aggatggtgt ccttgacggc tgcaaagacg aagcggatgt tctcggtatc
      661 tgtggcgcac gtgaagtggg agtagatgat tttgtcactg tcggggttca ggtccacgaa
      721 cattttcagg atgaattctc gagctgcctg ggcatctctc tggggtccat catattctgg
      781 gaagtagtcg actaggtggg aatacatgat tttctcctct agaagatctt tcttgtttaa
      841 gaacagaatc acagaggagt tctggaacca ggggtagg
//
