LOCUS       sequence-303064-        2301 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of secreted protein fused to 
            Fc(DAPA)-Avi-6xHis..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-SEMA3F-Fc(DAPA)-AviTag-6xHis
FEATURES             Location/Qualifiers
     source          1..2301
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2118..2126
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 agctttccca cacaagatca tctgcctgcc acacctagag tgcggctgag ctttaaagag
       61 ctgaaggcca ccggcacagc ccactttttc aactttctgc tgaacaccac cgactaccgc
      121 atcctgctga aggacgagga ccacgatcgt atgtacgtgg gcagcaagga ctacgtgctg
      181 agcctggacc tgcacgacat caacagagag cccctgatca tccattgggc cgcctctcca
      241 cagagaatcg aggaatgtgt gctgagcggc aaggacgtga acggcgagtg tggaaacttc
      301 gtcagactga tccagccttg gaacagaacc cacctgtacg tgtgtggcac cggcgcctac
      361 aatcctatgt gcacctacgt gaacagaggc cgcagagcac aggctacccc ttggacacag
      421 acacaggccg ttagaggaag aggctccaga gctacagatg gcgctctgag gcctatgcct
      481 accgctccta gacaggacta catcttctac ctggaacctg agcggctgga atccggcaag
      541 ggcaagtgtc cttacgaccc caagctggat acagccagcg ctctgatcaa cgaggaactg
      601 tatgccggcg tgtacatcga cttcatgggc accgatgccg ccatcttcag aaccctggga
      661 aagcagaccg ccatgcggac cgaccagtac aacagcagat ggctgaacga ccccagcttc
      721 atccacgccg agctgatccc tgatagcgcc gagagaaacg acgacaagct gtacttcttc
      781 ttccgcgaac gctctgccga ggctcctcag tctcctgccg tgtatgccag aatcggcaga
      841 atctgcctga acgacgatgg cggccactgc tgtctggtca acaagtggtc taccttcctg
      901 aaggcccggc tcgtttgttc tgtgcctggc gaggatggca tcgagacaca cttcgatgag
      961 ctgcaggacg tgttcgtgca gcagacccag gatgtgcgga atcctgtgat ctacgccgtg
     1021 tttacaagca gcggcagcgt gttcagaggc agcgccgtgt gtgtgtactc catggccgac
     1081 atcagaatgg tgttcaacgg ccccttcgct cacaaagagg gccccaatta ccagtggatg
     1141 cccttcagcg gcaagatgcc ctatcctaga cctggcacat gtcctggcgg caccttcaca
     1201 cctagcatga agtccaccaa ggattacccc gacgaagtga tcaacttcat gcggagccat
     1261 cctctgatgt accaggccgt gtatcccctg cagagaaggc ctctggttgt tagaacaggc
     1321 gccccttaca gactgaccac aatcgctgtg gatcaggtgg acgccgccga tggaagatac
     1381 gaggtgctgt tcctgggcac agacagaggc accgtgcaga aagtgatcgt gctgcccaag
     1441 gacgaccaag agctggaaga actgatgctg gaagaggtgg aagtgttcaa ggaccccgct
     1501 cctgtgaaaa ccatgaccat cagcagcaag cggcagcagc tgtatgtggc ttctgccgtg
     1561 ggagtgacac acctgtctct gcacagatgc caggcttatg gcgccgcttg tgccgattgc
     1621 tgtctcgcca gagatcctta ttgtgcctgg gatggccagg cctgtagcag atacacagcc
     1681 agctccaaga gaagatcccg cagacaggac gtgcggcacg gcaatcctat cagacagtgc
     1741 agaggcttca acagcaacgc caacaagaac gccgtggaaa gcgtgcagta tggcgtggcc
     1801 ggatctgccg cctttcttga gtgtcagcct agaagccctc aggccaccgt gaagtggctg
     1861 tttcagagag atcccggcga ccggcggaga gagattagag ccgaggatag attcctgaga
     1921 accgagcagg gactgctgct gagagcactg caactgagcg atagaggcct gtactcttgc
     1981 accgccaccg agaacaactt caagcacgtg gtcaccagag tccagctgca cgtgctggga
     2041 agagatgctg tgcatgccgc tctgttccct ccactgtcta tgtctgctcc tcctccacct
     2101 ggtgctggac ctccaacacc tccttatcaa gaactggccc agctgctggc tcagcctgaa
     2161 gtgggactga ttcaccagta ctgccaaggc tactggcgcc acgttccacc ttcacctaga
     2221 gaagctccag gcgctccaag aagccctgag cctcaggatc agaagaagcc cagaaacaga
     2281 cggcaccatc ctccagacac a
//
