LOCUS       sequence-303148-        2955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-ADGRF5-Fc(DAPA)-AviTag-6xHis
FEATURES             Location/Qualifiers
     source          1..2955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gctctgaatt ggaactacga gagcaccatc catcctctga gcctgcacga acatgagcct
       61 gctggcgaag aggccctgag acagaaaaga gccgtggcca caaagagccc caccgccgaa
      121 gagtacaccg tgaacatcga gatcagcttc gagaacgcca gctttctgga ccccatcaag
      181 gcctacctga acagcctgag cttccccatc cacggcaaca acaccgacca gatcaccgac
      241 atcctgagca tcaacgtgac caccgtgtgc agacctgccg gcaatgagat ctggtgtagc
      301 tgtgaaacag gctacggctg gcccagagag agatgcctgc acaacctgat ctgccaagag
      361 cgggatgtgt ttctgcctgg ccaccactgc agctgcctga aagaactgcc tcctaatggc
      421 cccttctgcc tgctgcaaga ggacgtgacc ctgaacatga gagtgcggct gaacgtgggc
      481 ttccaagagg acctgatgaa taccagcagc gccctgtaca gaagctacaa gaccgacctg
      541 gaaaccgcct tccggaaagg ctatggcatc ctgcctggct ttaagggcgt gaccgtgaca
      601 ggctttaaga gcggcagcgt ggtggtcacc tacgaagtga aaaccacacc tcctagcctg
      661 gaactgatcc acaaggccaa cgaacaggtg gtgcagagcc tgaaccagac ctacaagatg
      721 gactacaaca gcttccaggc cgtgaccatc aacgagagca atttcttcgt gaccccggaa
      781 atcatcttcg agggcgacac agtgtccctc gtgtgcgaga aagaggtgct gtctagtaac
      841 gtgtcctggc gctacgagga acagcagctg gaaatccaga acagctcccg gttcagcatc
      901 tacacagccc tgttcaacaa catgaccagc gtgtccaagc tgaccatcca caacattaca
      961 cctggcgacg ccggcgagta tgtgtgcaag ctgatcctgg atatcttcga gtacgagtgc
     1021 aagaaaaaga tcgacgtgat gcccatccag atcctcgcca acgaagagat gaaggtcatg
     1081 tgcgacaaca accccgtgtc tctgaactgc tgcagccagg gcaatgtgaa ctggtccaag
     1141 gtggaatgga agcaagaggg caagatcaac atccccggca cacccgagac agacatcgac
     1201 tccagctgta gcagatacac cctgaaggcc gatggcaccc agtgtcctag cggatcttct
     1261 ggcaccaccg tgatctacac atgcgagttc atcagcgcct acggcgccag aggcagcgcc
     1321 aatatcaaag tgacctttat ctccgtggcc aacctcacca tcacacccga tcctatctct
     1381 gtgtccgagg gccagaactt cagcatcaag tgcatcagcg acgtgtccaa ctacgacgag
     1441 gtgtactgga atacctccgc cggcatcaag atctaccagc ggttctacac caccagaaga
     1501 tacctggacg gcgccgagag cgtgctgacc gtgaaaacaa gcacaagaga gtggaacggc
     1561 acctaccact gcatcttccg gtacaagaac agctactcta tcgccaccaa ggacgtgatc
     1621 gtgcaccctc tgcctctgaa gctgaacatc atggtggacc ctctggaagc cacagtgtct
     1681 tgttctggct cccaccacat caagtgttgc atcgaagagg acggcgacta caaagtgaca
     1741 ttccacaccg gctcctcttc tctgcctgcc gccaaagaag tgaacaagaa gcaagtctgc
     1801 tataagcaca acttcaacgc ctcttccgtg tcatggtgca gcaagaccgt ggacgtgtgc
     1861 tgccacttta ccaacgccgc caacaatagc gtgtggtccc ctagcatgaa gctcaacctg
     1921 gtgcctggcg agaacatcac ctgtcaggac cctgtgattg gcgtgggcga gcctggaaaa
     1981 gtgatccaga agctgtgcag attcagcaac gtgccaagca gccctgagtc tcctatcggc
     2041 ggcaccatca cctataagtg tgtgggcagc cagtgggaag agaagcggaa cgattgcatc
     2101 tctgccccta tcaactccct gctgcagatg gccaaggcac tgatcaagag ccctagccag
     2161 gatgagatgc tgcccaccta tctgaaggac ctgtccatca gcatcgacaa ggccgagcac
     2221 gagatctctt ctagccctgg atctctgggc gccatcatca atatcctgga cctgctgagc
     2281 accgtgccta cacaagtgaa ctccgagatg atgacccacg tgctgtccac cgtgaatgtg
     2341 atcctgggca agcccgtgct gaacacctgg aaagttctcc agcagcagtg gaccaaccag
     2401 tcctctcagc tgctgcacag cgtggaaaga ttctctcagg ccctgcagtc tggcgatagc
     2461 cctccactga gcttcagcca gaccaacgtg cagatgagca gcatggtcat caagagcagt
     2521 caccccgaaa cctaccagca gagattcgtg ttcccctact tcgacctgtg gggcaacgtc
     2581 gtgatcgaca agagctatct ggaaaacctg cagagcgaca gcagcatcgt gaccatggcc
     2641 tttccaacac tgcaggccat tctggcccag gacatccaag agaacaactt tgccgagagc
     2701 ctggtcatga caaccactgt gtcccacaac accaccatgc cattccggat cagcatgacc
     2761 tttaagaaca acagcccctc tggcggcgag actaagtgcg tgttctggaa cttccggctg
     2821 gccaacaaca caggcggctg ggattctagc ggctgctatg ttgaagaagg cgacggcgat
     2881 aacgtgacct gcatctgtga tcacctgacc agcttctcca tcctgatgag ccctgacagc
     2941 cccgatccaa gctct
//
