LOCUS       sequence-303174-        3315 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-IGSF3-Fc(DAPA)-AviTag-6xHis
FEATURES             Location/Qualifiers
     source          1..3315
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 caaagacaag tgacagtgca agagggccca ctgtacagaa cagagggcag ccacatcacc
       61 atctggtgca acgtgtccgg ctatcagggc cccagcgagc agaatttcca gtggtccatc
      121 tacctgccta gcagccccga acgggaagtg cagatcgtgt ctacaatgga cagcagcttc
      181 ccctacgcca tctacaccca gagagtcaga ggcggcaaga tcttcatcga gagagtgcag
      241 ggcaacagca ccctgctgca catcacagat ctgcaggcca gagatgccgg cgagtacgag
      301 tgtcacaccc ctagcaccga caagcagtac ttcggcagct acagcgccaa gatgaacctg
      361 gtggtcatcc ccgatagcct gcagaccaca gccatgcctc agacactgca cagagtggaa
      421 caggaccctc tggaactgac atgcgaggtg gccagcgaga caatccagca ctctcatctg
      481 tctgtggcct ggctgagaca gaaagtgggc gagaagcccg tggaagtgat cagcctgagc
      541 cgggacttta tgctgcacag cagcagcgag tacgcccaga gacagagtct gggagaagtg
      601 cggctggaca agctgggcag aaccaccttc cggctgacca tcttccatct gcaacccagc
      661 gatcagggcg agttctactg tgaagccgcc gagtggattc aggaccccga tggatcttgg
      721 tacgccatga ccagaaagcg gagcgaaggc gccgtggtca atgtgcagcc taccgacaaa
      781 gaattcaccg tgcgcctgga aaccgagaag agactgcaca ccgtgggcga gcctgtggaa
      841 ttcagatgca tcctggaagc ccagaacgtg cccgacagat actttgccgt gtcctgggcc
      901 ttcaacagca gcctgattgc cacaatgggc cccaatgctg tgcccgtgct gaatagcgag
      961 tttgcccaca gagaggccag aggccagctg aaggtggcca aagaaagcga cagcgtgttc
     1021 gtgctgaaga tctaccacct gagacaagag gacagcggca agtacaactg cagagtgacc
     1081 gagcgggaaa agaccgtgac cggcgagttc atcgacaaag agagcaagcg gcccaagaac
     1141 atccccatca tcgtgctgcc tctgaagtcc agcatctctg tggaagtggc cagcaacgcc
     1201 agcgtgatcc tcgaaggcga ggacctgaga ttcagctgtt ctgtgcggac agccggcaga
     1261 cctcagggca gattttctgt gatctggcag ctggtggacc ggcagaacag acggtccaac
     1321 atcatgtggc tggacagaga tggcaccgtg cagccaggca gcagctactg ggagagaagc
     1381 tcttttggcg gcgtccagat ggaacaagtg cagcccaata gcttcagcct gggcatcttc
     1441 aactcccgga aagaggacga gggccagtac gaatgccacg tgacagagtg ggtccgagcc
     1501 gttgatggcg agtggcagat tgtgggagag agaagggcca gcacacccat ctctatcaca
     1561 gccctggaaa tgggcttcgc cgtgaccgcc attagcagaa cacctggcgt gacctacagc
     1621 gacagcttcg acctgcagtg catcatcaag cctcactacc ctgcctgggt gccagtgtcc
     1681 gtgacttgga gatttcagcc tgtgggcacc gtggaatttc acgacctggt caccttcaca
     1741 agagatggcg gagtgcagtg gggcgacaga agcagcagct ttagaaccag aaccgccatc
     1801 gagaaggccg agagcagcaa caatgtgcgg ctgagcatca gcagagccag cgatacagag
     1861 gccggaaagt accagtgtgt ggccgagctg tggcggaaga actacaacaa cacctggacc
     1921 agactggccg agcggaccag caatctgctg gaaatcagag tgctgcagcc cgtgaccaag
     1981 ctccaggtgt ccaagagcaa gagaaccctg acactggtgg aaaacaagcc catccagctg
     2041 aactgcagcg tgaagtccca gaccagccag aacagccatt tcgccgtgct ttggtacgtg
     2101 cacaagccct ctgatgccga cggcaagctg atcctgaaaa ccacacacaa cagcgccttt
     2161 gagtacggca cctacgccga ggaagaaggc cttagagcca gactgcagtt cgagcggcat
     2221 gtgtctggcg gcctgttttc tctgactgtg cagcgggccg aagtgtccga tagcggcagc
     2281 tattactgcc acgtggaaga atggctgctg agccccaact acgcctggta taagctggcc
     2341 gaagaggtgt ccggacggac cgaagtgact gtgaaacagc ccgacagcag actgaggctg
     2401 tctcaggctc agggcaatct gagcgtgctg gaaacaagac aggtgcagct ggaatgcgtg
     2461 gtgctgaaca gaaccagcat caccagccag ctgatggttg agtggttcgt gtggaagcct
     2521 aatcaccccg agagggaaac cgtggccaga ctgagtagag atgccacctt ccactatggc
     2581 gagcaggccg ctaagaacaa cctgaagggc agactgcacc tggaatcacc ctctccaggc
     2641 gtgtaccggc tgttcatcca gaatgtggcc gtgcaggact ctggcaccta cagctgtcat
     2701 gtcgaagagt ggctgccttc tccttccggc atgtggtaca agagagccga ggataccgcc
     2761 ggacagacag ccctgacagt gatgaggcct gatgccagcc tgcaggttga caccgtggtg
     2821 cctaatgcca ccgtgtctga gaaagccgcc ttccagctcg actgctccat cgtgtccaga
     2881 agctcccagg acagcagatt cgccgtggcc tggtacagcc tgagaacaaa agccggcgga
     2941 aagcggtcta gccctggcct ggaagaacaa gaggaagaga gggaagaaga agaagaagag
     3001 gacgacgacg acgatgacga ccccacagaa agaacagccc tgctgagcgt gggacccgat
     3061 gctgtgtttg gacctgaggg ctctccttgg gaggggagac tgagattcca gagactgagc
     3121 cccgtgctgt acagactgac agtgctgcaa gcctctcctc aggataccgg caactactcc
     3181 tgtcacgtcg aggaatggct cccaagtcct cagaaagagt ggtacaggct gaccgaagaa
     3241 gagagcgccc ctatcggcat tcgggtgctc gatacaagcc ccacactgca gagcattatc
     3301 tgcagcaacg acgct
//
