LOCUS       sequence-30356-e         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Tomishige M, Vale RD
  TITLE     Controlling kinesin by reversible disulfide cross-linking. 
            Identifying the motility-producing conformational change.
  JOURNAL   J Cell Biol. 2000 Nov 27. 151(5):1081-92.
  PUBMED    11086009
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2000 Nov 27. 151(5):1081-92."
COMMENT     Sequence Label: A337C
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(33..50)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(51..764)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             complement(51..764)
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(91..111)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     706..734
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 cgggcttgta gcagccggat ctgctcgagt taatggtgat gatggtgatg tttgtatagt
       61 tcatccatgc catgtgtaat cccagcagct gttacaaact caagaaggac catgtggtct
      121 ctcttttcgt tgggatcttt cgaaagggca gattgtgtgg acaggtaatg gttgtctggt
      181 aaaaggacag ggccatcgcc aattggagta ttttgttgat aatggtctgc tagttgaacg
      241 cttccatctt caatgttgtg tctaattttg aagttaactt tgattccatt cttttgtttg
      301 tctgccatga tgtatacatt gtgtgagtta tagttgtatt ccaatttgtg tccaagaatg
      361 tttccatctt ctttaaaatc aatacctttt aactcgattc tattaacaag ggtatcacct
      421 tcaaacttga cttcagcacg tgtcttgtag ttcccgtcat ctttgaaaaa tatagttctt
      481 tcctgtacat aaccttcggg catggcactc ttgaaaaagt catgctgttt catatgatct
      541 gggtatcttg aaaagcattg aacaccataa gtcagagtag tgacaagtgt tggccatgga
      601 acaggtagtt ttccagtagt gcaaataaat ttaagggtaa gttttccgta tgttgcatca
      661 ccttcaccct ctccactgac agaaaatttg tgcccattaa catcaccatc taattcaaca
      721 agaattggga caactccagt gaaaagttct tctcctttac tcatggtacc ttttagtaaa
      781 gatgccatca tctcagctgc tcgttttttc tggtggttgg tcatttcctt aagtttctga
      841 agctcagcat ctatactcgc taaagttgcc gatttctgat tcaattcatc actaagcaat
      901 tca
//
