LOCUS       sequence-30386-e         757 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Tomishige M, Vale RD
  TITLE     Controlling kinesin by reversible disulfide cross-linking. 
            Identifying the motility-producing conformational change.
  JOURNAL   J Cell Biol. 2000 Nov 27. 151(5):1081-92.
  PUBMED    11086009
REFERENCE   2  (bases 1 to 757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2000 Nov 27. 151(5):1081-92."
COMMENT     Sequence Label: K339CLM S330C
FEATURES             Location/Qualifiers
     source          1..757
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(42..59)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(100..120)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     715..743
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 cttctttcgg gctttgttag cagccggatc tgctcgagtt aatggtgatg atggtgatgt
       61 ttgtatagtt catccatgcc atgtgtaatc ccagcagctg ttacaaactc aagaaggacc
      121 atgtggtctc tcttttcgtt gggatctttc gaaagggcag attgtgtgga caggtaatgg
      181 ttgtctggta aaaggacagg gccatcgcca attggagtat tttgttgata atggtctgct
      241 agttgaacgc ttccatcttc aatgttgtgt ctaattttga agttaacttt gattccattc
      301 ttttgtttgt ctgccatgat gtatacattg tgtgagttat agttgtattc caatttgtgt
      361 ccaagaatgt ttccatcttc tttaaaatca atacctttta actcgattct attaacaagg
      421 gtatcacctt caaacttgac ttcagcacgt gtcttgtagt tcccgtcatc tttgaaaaat
      481 atagttcttt cctgtacata accttcgggc atggcactct tgaaaaagtc atgctgtttc
      541 atatgatctg ggtatcttga aaagcattga acaccataag tcagagtagt gacaagtgtt
      601 ggccatggaa caggtagttt tccagtagtg caaataaatt taagggtaag ttttccgtat
      661 gttgcatcac cttcaccctc tccactgaca gaaaatttgt gcccattaac atcaccatct
      721 aattcaacaa gaattgggac aactccagtg aaaaggt
//
