LOCUS       sequence-304124-        2022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-PTK7-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..2022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1998..2006
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 gccatcgtgt ttatcaagca gcctagcagc caggacgccc tgcaaggccg tagagcactg
       61 ctgagatgcg aagtggaagc ccctggacct gtgcacgttt actggttgct tgatggcgcc
      121 cctgtgcagg acaccgagag aagatttgcc cagggcagca gcctgtcttt cgccgctgtt
      181 gacagactgc aggacagcgg cacctttcag tgcgtggcca gagatgatgt gaccggcgag
      241 gaagccagaa gcgccaacgc cagcttcaac atcaagtgga tcgaggctgg ccccgtggtg
      301 ctgaaacatc ctgcctctga ggccgagatc cagccacaga cacaagtgac cctgcggtgc
      361 cacatcgacg gacaccctag accaacctac cagtggttca gagatggcac ccctctgtcc
      421 gacggccaga gcaatcatac cgtgtccagc aaagagcgga acctgacact gaggcctgcc
      481 ggacctgaac acagcggcct gtattcttgt tgtgcccaca gcgcctttgg ccaggcctgt
      541 agctctcaga acttcaccct gagcattgcc gacgagagct tcgccagagt tgtgctggct
      601 cctcaggatg tggtggtggc cagatacgaa gaggccatgt tccactgcca gttcagcgct
      661 cagcctcctc catctctgca gtggctgttc gaggacgaga cacccatcac caacagaagc
      721 cggcctccac atctgagaag ggccaccgtg tttgccaatg gcagcctgct gctgactcaa
      781 gtgcggccta gaaacgccgg catctacaga tgtatcggcc agggccaaag aggccctcct
      841 atcatcctgg aagccacact gcacctggcc gagattgagg acatgcccct gttcgagccc
      901 agagtgttta cagccggcag cgaggaaaga gtgacctgcc tgcctcctaa gggactgcct
      961 gagccttctg tttggtggga acacgctggc gtcagactgc ctacacacgg cagagtgtac
     1021 cagaaaggcc acgaactggt gctggccaat atcgccgaat ccgacgcagg cgtgtacaca
     1081 tgtcatgccg ccaatctggc cggacagaga aggcaggacg tgaacatcac agtggccaca
     1141 gtgcccagct ggctgaagaa gcctcaggac tctcagctgg aagagggcaa gcctggctac
     1201 ctggattgtc tgacacaggc cacacctaag cctaccgtcg tgtggtacag aaaccagatg
     1261 ctgatctccg aggacagcag attcgaggtg ttcaagaacg gcaccctgcg gatcaacagc
     1321 gtggaagtgt acgacggcac ctggtacaga tgcatgagca gcacaccagc cggctccatt
     1381 gaagcccagg ctagagtgca ggtcctggaa aagctgaagt tcacccctcc acctcagcca
     1441 cagcagtgca tggaattcga caaagaggct acagtcccct gcagcgccac aggcagagag
     1501 aagcctacaa tcaagtggga gagagccgac ggcagctccc tgcctgaatg ggtcacagat
     1561 aatgccggca ctctgcactt tgccagagtg accagggacg acgccggcaa ttatacctgt
     1621 atcgccagca atggcccaca gggccagatt agagcccacg tgcagctgac agtggccgtg
     1681 ttcatcacct tcaaggtgga acccgagcgg accaccgtgt atcagggaca tacagctctg
     1741 ctgcagtgcg aggctcaggg cgatcctaag cctctgatcc agtggaaggg caaagaccgg
     1801 attctggacc ccacaaagct gggccccaga atgcacatct tccagaacgg ctccctcgtg
     1861 atccacgatg tggcccctga agatagcggc cggtacacct gtattgccgg aaacagctgc
     1921 aatatcaagc acacagaggc ccctctgtac gtggtggaca agcctgtgcc tgaggaatct
     1981 gaaggccctg gcagcccacc tccttacaag atgatccaga ca
//
