LOCUS       sequence-304317-       11425 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein partial extracellular 
            domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11425)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 11425)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-NOTCH4(Trunc1)-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..11425
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             7030..7065
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             7075..7098
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             8758..8802
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             8803..8829
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(8969..8986)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8975..9086
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9204..9224)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9209..9538
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9389..9524
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9451..9470
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9586..9633
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9652..10251
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9652..9671)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10148..10168
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    10381..10502
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10418..10437)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10472..10491
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(10555..11346,11347..11415))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11178..11197
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(11416..11425,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgccag aggactgctg
     2761 tgtggatctt ttccagagcc ttgcgccaat ggcggcacat gtctgtctct gtctctcggc
     2821 cagggaacct gtcagtgtgc tcctggattt ctgggcgaga cttgtcagtt ccccgatcct
     2881 tgtcagaacg cccagctgtg tcagaatggc ggctcttgtc aagctctgct gcctgctcct
     2941 ctgggactgc cttcttctcc atctccactg acacccagct ttctgtgcac ctgtctgcct
     3001 ggctttaccg gcgaaagatg ccaggccaag ctggaagatc cctgtcctcc tagcttctgc
     3061 agcaagaggg gcagatgtca catccaggca agcggcagac ctcagtgttc ttgtatgcct
     3121 ggctggacag gcgagcagtg ccagctgaga gatttctgca gcgccaatcc ttgtgtgaac
     3181 ggcggcgtgt gtctggccac atatcctcag atccagtgcc actgtcctcc aggctttgag
     3241 ggacacgcct gcgagaggga tgtgaacgag tgttttcagg accccggacc ttgtcctaag
     3301 ggcacctctt gtcacaacac cctgggcagc ttccagtgtc tgtgtcctgt gggacaagag
     3361 ggacccagat gtgaactgag agccgggcct tgtccaccta gaggctgttc taatggcggg
     3421 acctgtcagc tgatgcccga gaaggatagc accttccacc tgtgtctgtg cccacctggc
     3481 ttcatcggcc ccgattgcga agtgaacccc gacaattgtg tgtcccacca gtgccagaac
     3541 ggcggaactt gtcaggacgg cctggacacc tatacctgcc tgtgtccaga gacatggacc
     3601 ggctgggatt gcagcgagga tgtggatgag tgtgaaacac agggccctcc acactgcaga
     3661 aacggcggga catgccagaa tagcgccggc tcttttcact gcgtgtgtgt gtcaggctgg
     3721 ggcggcacaa gctgcgagga aaatctggac gattgcattg ccgccacatg cgcccctggc
     3781 agcacatgta ttgatagagt gggctccttc agctgtctct gccctcctgg aagaacaggc
     3841 ctgctgtgtc acctggaaga tatgtgcctg agccagcctt gtcatggcga cgcccagtgt
     3901 agcacaaatc ctctgaccgg cagcaccctg tgtctttgtc agcctggcta ctctggccct
     3961 acctgccacc aggatctgga tgaatgtctg atggcccagc agggcccttc tccttgtgaa
     4021 catggcggaa gctgtctgaa cacacccggc agcttcaact gcttgtgtcc tcctggctac
     4081 accggaagca gatgcgaggc cgatcacaac gaatgcctgt ctcagccttg ccatcctggc
     4141 tctacctgtc tggatctgct ggccaccttt cactgcctct gtccgcctgg actggaagga
     4201 cagctgtgcg aggtggaaac aaatgagtgc gccagcgctc cttgcctgaa tcacgccgat
     4261 tgccacgacc tgctgaacgg ctttcagtgt atctgcctgc ctggattcag cggcaccaga
     4321 tgcgaagagg acatcgacga gtgtagaagc agcccttgtg ccaacggtgg ccagtgtcaa
     4381 gatcagccag gcgccttcca ctgcaagtgt ctgccaggat ttgaaggccc cagatgccag
     4441 accgaagtgg acgagtgtct gagcgatcca tgtccagtgg gagcctcttg tcttgacctg
     4501 cctggcgcct tcttttgtct ttgccctagc ggctttacag ggcagctgtg tgaagtgccc
     4561 ctgtgcgctc ctaatctgtg ccagcctaaa cagatctgca aggaccagaa ggataaggcc
     4621 aactgccttt gtcctgatgg ctctccagga tgcgccccac ctgaggataa ttgcacatgt
     4681 caccacggcc actgccagag aagctcttgt gtgtgtgatg tcggatggac aggacccgag
     4741 tgtgaagccg aactcggcgg atgtattagc gccccatgtg ctcacggtgg cacctgttat
     4801 ccacagccaa gcggctacaa ctgcacatgc cctacaggct acacaggccc aacctgctct
     4861 gaggaaatga cagcctgtca cagcggccca tgccttaacg gcgggtcttg taatccttct
     4921 cctggcggct actactgtac ctgtccacca tctcacaccg ggcctcagtg tcagacaagc
     4981 accgactact gtgtgtctgc cccttgcttt aacggtggaa cctgcgtgaa cagacctggc
     5041 acattctctt gcctgtgcgc catgggcttt caggggccta gatgtgaagg caagctgagg
     5101 ccttcctgcg ccgactctcc ctgtagaaat agagccacct gtcaggacag ccctcagggc
     5161 ccaagatgtt tgtgtccaac cggctatacc ggcggcagct gtcagacact gatggacctg
     5221 tgtgcccaga agccttgtcc tcggaatagc cactgtctgc aaacaggccc ctccttccat
     5281 tgcctctgcc tgcaaggatg gaccgggcca ctgtgtaatc tgcctctcag ctcctgtcag
     5341 aaggccgcac tgtctcaggg catcgatgtg tccagtctct gccacaatgg cggactgtgt
     5401 gttgatagcg gccccagcta cttctgtcac tgcccaccag gttttcaggg ctccctgtgt
     5461 caggatcacg tgaacccttg cgagagcaga ccttgccaga atggggccac ttgtatggct
     5521 cagccttctg gctatctgtg tcagtgcgcc ccaggctacg atggccagaa ttgcagcaaa
     5581 gagctggacg cctgtcagag ccagccatgc cacaatcacg gcacatgcac acctaagcct
     5641 ggcggatttc actgtgcatg ccctccagga ttcgtgggac ttcgctgtga aggcgacgtt
     5701 gacgaatgtc tggaccagcc atgtcaccct actggaacag ccgcctgtca ttctctggcc
     5761 aacgccttct attgccagtg tctccctgga cacacaggcc agtggtgcga ggtcgagatt
     5821 gatccttgcc actctcagcc ctgctttcac ggcggtacat gtgaagctac agccggctct
     5881 cccctgggct tcatttgtca ctgtcccaag ggattcgagg gccccacatg ttctcacaga
     5941 gcccctagct gtggcttcca ccactgtcat cacggcggac tctgtctgcc atctcctaag
     6001 ccaggcttcc cacctagatg cgcctgtctc tctggatatg gcggccctga ttgtctgacc
     6061 cctcctgctc ctaaaggctg tggccctcct tctccatgcc tgtacaatgg cagctgtagc
     6121 gagacaacag gcctcggcgg accaggcttt agatgtagct gtcctcacag cagccctgga
     6181 ccaaggtgtc agaaacctgg cgctaagggc tgcgaaggca gatctggcga cggtgcatgt
     6241 gatgctggat gttctggacc tggcggaaac tgggatggcg gagattgctc tctgggagtc
     6301 cctgatcctt ggaagggatg ccctagccac agcagatgct ggctgctgtt cagagatggc
     6361 cagtgccatc ctcagtgcga cagcgaagag tgcctgttcg atggctacga ctgcgagaca
     6421 ccaccagcct gtacacccgc ctacgatcag tactgccacg atcacttcca caacggacac
     6481 tgcgagaagg gctgcaatac cgccgaatgt ggatgggacg gtggcgattg cagaccagag
     6541 gatggcgatc ctgaatgggg accttctctc gctctgctgg ttgtgcttag ccctccagct
     6601 ctggatcagc agctgtttgc tctggctaga gtgctgagcc tgactctgag agttggcctg
     6661 tgggtccgaa aggaccgcga cggcagagat atggtgtacc cttatccagg cgccagagcc
     6721 gaggaaaagc tcggcggaac aagagatccc acctaccaag aaagagccgc tcctcagaca
     6781 cagcccctgg gcaaagagac agattccctg agcgccggat ttgtggtggt catgggagtc
     6841 gatctgtcca gatgtggacc tgatcacccc gccagtagat gtccttggga ccctggactg
     6901 ctgctgagat tccttgctgc tatggctgcc gtgggagcac ttgaaccatt gctgcctgga
     6961 ccactgctgg ctgtgcatcc tcatgctgga accgctcctc cagctaacca gcttccttgg
     7021 ggcgcgccgg aagatcaagt tgatccaaga ttgattgatg gcaagggcgg atctctggag
     7081 gtgctgttcc agggaccagg cggaagcggc aatcccggcg gcgatctggc cccccagatg
     7141 ctgcgcgagc tgcaggagac caatgccgcc ctgcaggacg tgcgcgagct gctgcgccac
     7201 cgcgtgaagg agatcacctt cctgaagaac accgtgatgg agtgcgacgc ctgcggtatg
     7261 cagccctcta gaggctccgg ctccatcatc ccagttgagg aggagaaccc ggacttctgg
     7321 aaccgcgagg cagccgaggc cctgggtgcc gccaagaagc tgcagcctgc acagacagcc
     7381 gccaagaacc tcatcatctt cctgggcgat gggatggggg tgtctacggt gacagctgcc
     7441 aggatcctaa aagggcagaa gaaggacaaa ctggggcctg agatacccct ggccatggac
     7501 cgcttcccat atgtggctct gtccaagaca tacaatgtag acaaacatgt gccagacagt
     7561 ggagccacag ccacggccta cctgtgcggg gtcaagggca acttccagac cattggcttg
     7621 agtgcagccg cccgctttaa ccagtgcaac acgacacgcg gcaacgaggt catctccgtg
     7681 atgaatcggg ccaagaaagc agggaagtca gtgggagtgg taaccaccac acgagtgcag
     7741 cacgcctcgc cagccggcac ctacgcccac acggtgaacc gcaactggta ctcggacgcc
     7801 gacgtgcctg cctcggcccg ccaggagggg tgccaggaca tcgctacgca gctcatctcc
     7861 aacatggaca ttgacgtgat cctaggtgga ggccgaaagt acatgtttcg catgggaacc
     7921 ccagaccctg agtacccaga tgactacagc caaggtggga ccaggctgga cgggaagaat
     7981 ctggtgcagg aatggctggc gaagcgccag ggtgcccggt atgtgtggaa ccgcactgag
     8041 ctcatgcagg cttccctgga cccgtctgtg acccatctca tgggtctctt tgagcctgga
     8101 gacatgaaat acgagatcca ccgagactcc acactggacc cctccctgat ggagatgaca
     8161 gaggctgccc tgcgcctgct gagcaggaac ccccgcggct tcttcctctt cgtggagggt
     8221 ggtcgcatcg accatggtca tcatgaaagc agggcttacc gggcactgac tgagacgatc
     8281 atgttcgacg acgccattga gagggcgggc cagctcacca gcgaggagga cacgctgagc
     8341 ctcgtcactg ccgaccactc ccacgtcttc tccttcggag gctaccccct gcgagggagc
     8401 tccatcttcg ggctggcccc tggcaaggcc cgggacagga aggcctacac ggtcctccta
     8461 tacggaaacg gtccaggcta tgtgctcaag gacggcgccc ggccggatgt taccgagagc
     8521 gagagcggga gccccgagta tcggcagcag tcagcagtgc ccctggacga agagacccac
     8581 gcaggcgagg acgtggcggt gttcgcgcgc ggcccgcagg cgcacctggt tcacggcgtg
     8641 caggagcaga ccttcatagc gcacgtcatg gccttcgccg cctgcctgga gccctacacc
     8701 gcctgcgacc tggcgccccc cgccggcacc accgacgccg cgcacccggg tggcggagga
     8761 ctgaacgaca tcttcgaggc tcagaaaatc gaatggcacg aacatcacca tcaccatcac
     8821 catcaccatt aaggccggcc tgtagtcact ggcacaacgc gttacccggt aagccaatcg
     8881 ggtatacacg gtcgtcatac tgcagacagg gttcttctac tttgcaagat agtcttgagt
     8941 agtaaaataa atagatagag aaaatcagcc tcgactgtgc cttctagttg ccagccatct
     9001 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     9061 tcctaataaa atgaggaaat tgcatcacaa cactcaaccc tatctcggtc tattcttttg
     9121 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     9181 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     9241 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     9301 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     9361 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     9421 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     9481 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     9541 gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca
     9601 tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac catgaccgag
     9661 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
     9721 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
     9781 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     9841 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
     9901 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     9961 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
    10021 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
    10081 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacatcc
    10141 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
    10201 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acacgtgcta
    10261 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
    10321 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
    10381 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
    10441 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
    10501 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcattacca
    10561 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
    10621 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagcgc
    10681 tgcgatgata ccgcgagaac cacgctcacc ggctccggat ttatcagcaa taaaccagcc
    10741 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
    10801 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
    10861 tgccatcgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
    10921 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
    10981 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
    11041 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
    11101 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    11161 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    11221 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    11281 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    11341 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    11401 atgttgaata ctcatattct tcctt
//
