LOCUS       sequence-304358-        1185 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian cell surface expression of His- and FLAG-tagged 
            full-length protein for binding and signaling assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 1185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pTT3-SP-6xHis-KIR2DL4(FL)-FLAG
FEATURES             Location/Qualifiers
     source          1..1185
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             76..96
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             1162..1185
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgtctatga gccctacagt gatcatcctg gcctgcctgg gattcttcct ggatcagtct
       61 gtgtgggccg gctctcacca ccatcaccat catcacgttg gcggccagga caagcctttt
      121 tgttctgcct ggcctagcgc tgtggtgcct caaggtggac acgtgaccct gagatgccac
      181 tacagacggg gcttcaacat cttcaccctg tacaagaaag acggcgtgcc cgtgcctgag
      241 ctgtacaaca gaatcttctg gaacagcttc ctgatcagcc ccgtgacacc agctcacgcc
      301 ggcacataca gatgcagagg ctttcaccct cacagcccca cagagtggtc cgctccatct
      361 aaccctctgg tcatcatggt caccggcctg tacgagaagc ctagcctgac agctagaccc
      421 ggacctacag ttagagccgg cgagaatgtg acactgagct gcagcagcca gagcagcttc
      481 gacatctacc acctgagcag agagggcgaa gcccacgaac ttagactgcc tgccgtgcct
      541 agcatcaacg gcacctttca ggccgatttt ccactgggac ctgccacaca cggcgagact
      601 tacagatgct ttggcagctt ccacggcagc ccttacgagt ggtctgatcc tagcgatcct
      661 ctgcctgtgt ccgtgacagg caatcctagc agcagctggc cttctccaac cgagcctagc
      721 tttaagaccg gaatcgccag acatctgcac gccgtgatca gatacagcgt ggccatcatc
      781 ctgtttacga tcctgccttt cttcctgctg caccggtggt gctccaagaa aaaggacgcc
      841 gccgtgatga atcaagagcc tgccggccac agaaccgtga acagagaaga tagcgacgag
      901 caggaccctc aagaagtgac atacgcccag ctggaccact gcatctttac ccagagaaag
      961 atcacaggcc ccagccagag aagcaagagg ccttctaccg ataccagcgt gtgcatcgag
     1021 ctgcccaatg ccgaacctag agcactgagc cctgctcacg aacaccattc tcaggccctg
     1081 atgggcagca gcagagagac aacagctctg tctcagaccc agctggcttc ctctaatgtg
     1141 cctgccgccg gaatcggcag cgactacaag gacgacgacg acaag
//
