LOCUS       sequence-304641-       10412 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-to-T base editor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10412)
  AUTHORS   Arbab M, Shen MW, Mok B, Wilson C, Matuszek Z, Cassa CA, Liu DR
  TITLE     Determinants of Base Editing Outcomes from Target Library Analysis 
            and Machine Learning.
  JOURNAL   Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 
            10.1016/j.cell.2020.05.037.
  PUBMED    32533916
REFERENCE   2  (bases 1 to 10412)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2020 
            Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037."
COMMENT     Sequence Label: p2T-CMV-CDAmax-BlastR
FEATURES             Location/Qualifiers
     source          1..10412
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             116..136
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             140..760
                     /codon_start=1
                     /product="cytidine deaminase from the sea lamprey 
                     Petromyzon marinus (Rogozin et al., 2007)"
                     /label=PmCDA1
                     /translation="TDAEYVRIHEKLDIYTFKKQFFNNKKSVSHRCYVLFELKRRGERR
                     ACFWGYAVNKPQSGTERGIHAEIFSIRKVEEYLRDNPGQFTINWYSSWSPCADCAEKIL
                     EWYNQELRGNGHTLKIWACKLYYEKNARNQIGLWNLRDNGVGLNVMVSEHYQCCRKIFI
                     QSSHNQLNENRWLEKTLKRAEKRRSELSIMIQVKILHTTKSPAV"
     CDS             join(857..880,881..883,884..4957)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             857..4957
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4988..5236
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5267..5515
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5558..5578
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5587..5604
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5627..5644)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5633..5857
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5928..5944)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5928..5944)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5941..5963)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5952..5968
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5976..5982,5983..6000,6001..6006))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6021..6042
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    6159..6280
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6170..6189)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6224..6243
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(6438..6836)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(6855..6902)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(6950..7279)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6964..7099
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7018..7037)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     7264..7284
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     misc_feature    join(7403..7590,7591..7602)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     rep_origin      complement(7663..8251)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7743..7762)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8422..9213,9214..9282))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9045..9064
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9283..9387)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9466..9485)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(9677..9688,9689..9826)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     enhancer        9835..10214
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        join(10215..10412,1..6)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10369..10389
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 agagctggtt tagtgaaccg tcagatccgc tagagatccg cggccgctaa tacgactcac
       61 tatagggaga gccgccacca tgaaacggac agccgacgga agcgagttcg agtcaccaaa
      121 gaagaagcgg aaagtcagta ccgacgccga gtacgtgcgg atccacgaga agctggatat
      181 ctacacattc aagaagcagt tctttaacaa caagaagtcc gtgtctcaca gatgctacgt
      241 gctgttcgag ctgaagcgga gaggagagag gcgcgcctgt ttttggggct atgccgtgaa
      301 caagccacag tctggaaccg agaggggaat ccacgcagag atcttcagca tcaggaaggt
      361 ggaggagtac ctgcgcgaca accccggcca gtttacaatc aattggtata gctcctggag
      421 cccttgcgcc gattgtgccg agaagatcct ggagtggtac aaccaggagc tgaggggcaa
      481 tggccacacc ctgaagatct gggcctgcaa gctgtactat gagaagaacg ccaggaatca
      541 gatcggcctg tggaacctgc gcgacaatgg cgtgggcctg aacgtgatgg tgtccgagca
      601 ctatcagtgc tgtcgcaaga tctttatcca gtctagccac aatcagctga acgagaatcg
      661 gtggctggag aagacactga agagagccga gaagcggaga agcgagctgt ccatcatgat
      721 ccaggtgaag atcctgcaca ccacaaagtc tcccgccgtg tctggcggat ctagcggagg
      781 atcctctggc agcgagacac caggaacaag cgagtcagca acaccagaga gcagtggcgg
      841 cagcagcggc ggcagcgaca agaagtacag catcggcctg gccatcggca ccaactctgt
      901 gggctgggcc gtgatcaccg acgagtacaa ggtgcccagc aagaaattca aggtgctggg
      961 caacaccgac cggcacagca tcaagaagaa cctgatcgga gccctgctgt tcgacagcgg
     1021 cgaaacagcc gaggccaccc ggctgaagag aaccgccaga agaagataca ccagacggaa
     1081 gaaccggatc tgctatctgc aagagatctt cagcaacgag atggccaagg tggacgacag
     1141 cttcttccac agactggaag agtccttcct ggtggaagag gataagaagc acgagcggca
     1201 ccccatcttc ggcaacatcg tggacgaggt ggcctaccac gagaagtacc ccaccatcta
     1261 ccacctgaga aagaaactgg tggacagcac cgacaaggcc gacctgcggc tgatctatct
     1321 ggccctggcc cacatgatca agttccgggg ccacttcctg atcgagggcg acctgaaccc
     1381 cgacaacagc gacgtggaca agctgttcat ccagctggtg cagacctaca accagctgtt
     1441 cgaggaaaac cccatcaacg ccagcggcgt ggacgccaag gccatcctgt ctgccagact
     1501 gagcaagagc agacggctgg aaaatctgat cgcccagctg cccggcgaga agaagaatgg
     1561 cctgttcgga aacctgattg ccctgagcct gggcctgacc cccaacttca agagcaactt
     1621 cgacctggcc gaggatgcca aactgcagct gagcaaggac acctacgacg acgacctgga
     1681 caacctgctg gcccagatcg gcgaccagta cgccgacctg tttctggccg ccaagaacct
     1741 gtccgacgcc atcctgctga gcgacatcct gagagtgaac accgagatca ccaaggcccc
     1801 cctgagcgcc tctatgatca agagatacga cgagcaccac caggacctga ccctgctgaa
     1861 agctctcgtg cggcagcagc tgcctgagaa gtacaaagag attttcttcg accagagcaa
     1921 gaacggctac gccggctaca ttgacggcgg agccagccag gaagagttct acaagttcat
     1981 caagcccatc ctggaaaaga tggacggcac cgaggaactg ctcgtgaagc tgaacagaga
     2041 ggacctgctg cggaagcagc ggaccttcga caacggcagc atcccccacc agatccacct
     2101 gggagagctg cacgccattc tgcggcggca ggaagatttt tacccattcc tgaaggacaa
     2161 ccgggaaaag atcgagaaga tcctgacctt ccgcatcccc tactacgtgg gccctctggc
     2221 caggggaaac agcagattcg cctggatgac cagaaagagc gaggaaacca tcaccccctg
     2281 gaacttcgag gaagtggtgg acaagggcgc ttccgcccag agcttcatcg agcggatgac
     2341 caacttcgat aagaacctgc ccaacgagaa ggtgctgccc aagcacagcc tgctgtacga
     2401 gtacttcacc gtgtataacg agctgaccaa agtgaaatac gtgaccgagg gaatgagaaa
     2461 gcccgccttc ctgagcggcg agcagaaaaa ggccatcgtg gacctgctgt tcaagaccaa
     2521 ccggaaagtg accgtgaagc agctgaaaga ggactacttc aagaaaatcg agtgcttcga
     2581 ctccgtggaa atctccggcg tggaagatcg gttcaacgcc tccctgggca cataccacga
     2641 tctgctgaaa attatcaagg acaaggactt cctggacaat gaggaaaacg aggacattct
     2701 ggaagatatc gtgctgaccc tgacactgtt tgaggacaga gagatgatcg aggaacggct
     2761 gaaaacctat gcccacctgt tcgacgacaa agtgatgaag cagctgaagc ggcggagata
     2821 caccggctgg ggcaggctga gccggaagct gatcaacggc atccgggaca agcagtccgg
     2881 caagacaatc ctggatttcc tgaagtccga cggcttcgcc aacagaaact tcatgcagct
     2941 gatccacgac gacagcctga cctttaaaga ggacatccag aaagcccagg tgtccggcca
     3001 gggcgatagc ctgcacgagc acattgccaa tctggccggc agccccgcca ttaagaaggg
     3061 catcctgcag acagtgaagg tggtggacga gctcgtgaaa gtgatgggcc ggcacaagcc
     3121 cgagaacatc gtgatcgaaa tggccagaga gaaccagacc acccagaagg gacagaagaa
     3181 cagccgcgag agaatgaagc ggatcgaaga gggcatcaaa gagctgggca gccagatcct
     3241 gaaagaacac cccgtggaaa acacccagct gcagaacgag aagctgtacc tgtactacct
     3301 gcagaatggg cgggatatgt acgtggacca ggaactggac atcaaccggc tgtccgacta
     3361 cgatgtggac catatcgtgc ctcagagctt tctgaaggac gactccatcg acaacaaggt
     3421 gctgaccaga agcgacaaga accggggcaa gagcgacaac gtgccctccg aagaggtcgt
     3481 gaagaagatg aagaactact ggcggcagct gctgaacgcc aagctgatta cccagagaaa
     3541 gttcgacaat ctgaccaagg ccgagagagg cggcctgagc gaactggata aggccggctt
     3601 catcaagaga cagctggtgg aaacccggca gatcacaaag cacgtggcac agatcctgga
     3661 ctcccggatg aacactaagt acgacgagaa tgacaagctg atccgggaag tgaaagtgat
     3721 caccctgaag tccaagctgg tgtccgattt ccggaaggat ttccagtttt acaaagtgcg
     3781 cgagatcaac aactaccacc acgcccacga cgcctacctg aacgccgtcg tgggaaccgc
     3841 cctgatcaaa aagtacccta agctggaaag cgagttcgtg tacggcgact acaaggtgta
     3901 cgacgtgcgg aagatgatcg ccaagagcga gcaggaaatc ggcaaggcta ccgccaagta
     3961 cttcttctac agcaacatca tgaacttttt caagaccgag attaccctgg ccaacggcga
     4021 gatccggaag cggcctctga tcgagacaaa cggcgaaacc ggggagatcg tgtgggataa
     4081 gggccgggat tttgccaccg tgcggaaagt gctgagcatg ccccaagtga atatcgtgaa
     4141 aaagaccgag gtgcagacag gcggcttcag caaagagtct atcctgccca agaggaacag
     4201 cgataagctg atcgccagaa agaaggactg ggaccctaag aagtacggcg gcttcgacag
     4261 ccccaccgtg gcctattctg tgctggtggt ggccaaagtg gaaaagggca agtccaagaa
     4321 actgaagagt gtgaaagagc tgctggggat caccatcatg gaaagaagca gcttcgagaa
     4381 gaatcccatc gactttctgg aagccaaggg ctacaaagaa gtgaaaaagg acctgatcat
     4441 caagctgcct aagtactccc tgttcgagct ggaaaacggc cggaagagaa tgctggcctc
     4501 tgccggcgaa ctgcagaagg gaaacgaact ggccctgccc tccaaatatg tgaacttcct
     4561 gtacctggcc agccactatg agaagctgaa gggctccccc gaggataatg agcagaaaca
     4621 gctgtttgtg gaacagcaca agcactacct ggacgagatc atcgagcaga tcagcgagtt
     4681 ctccaagaga gtgatcctgg ccgacgctaa tctggacaaa gtgctgtccg cctacaacaa
     4741 gcaccgggat aagcccatca gagagcaggc cgagaatatc atccacctgt ttaccctgac
     4801 caatctggga gcccctgccg ccttcaagta ctttgacacc accatcgacc ggaagaggta
     4861 caccagcacc aaagaggtgc tggacgccac cctgatccac cagagcatca ccggcctgta
     4921 cgagacacgg atcgacctgt ctcagctggg aggtgacagc ggcgggagcg gcgggagcgg
     4981 ggggagcact aatctgagcg acatcattga gaaggagact gggaaacagc tggtcattca
     5041 ggagtccatc ctgatgctgc ctgaggaggt ggaggaagtg atcggcaaca agccagagtc
     5101 tgacatcctg gtgcacaccg cctacgacga gtccacagat gagaatgtga tgctgctgac
     5161 ctctgacgcc cccgagtata agccttgggc cctggtcatc caggattcta acggcgagaa
     5221 taagatcaag atgctgagcg gaggatccgg aggatctgga ggcagcacca acctgtctga
     5281 catcatcgag aaggagacag gcaagcagct ggtcatccag gagagcatcc tgatgctgcc
     5341 cgaagaagtc gaagaagtga tcggaaacaa gcctgagagc gatatcctgg tccataccgc
     5401 ctacgacgag agtaccgacg aaaatgtgat gctgctgaca tccgacgccc cagagtataa
     5461 gccctgggct ctggtcatcc aggattccaa cggagagaac aaaatcaaaa tgctgtctgg
     5521 cggctcaaaa agaaccgccg acggcagcga attcgagccc aagaagaaga ggaaagtcta
     5581 accggtcatc atcaccatca ccattgagtt taaacccgct gatcagcctc gactgtgcct
     5641 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     5701 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     5761 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     5821 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     5881 tggggctcga taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     5941 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     6001 tgtaaagcct agggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     6061 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     6121 gggagaggcg gtttgcgtat tgggcgctac agacatgata agatacattg atgagtttgg
     6181 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     6241 tgctttattt gtaaccatta taagctgcaa taaacaagtt ggggtgggcg aagaactcca
     6301 gcatgagatc cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg attccgaagc
     6361 ccaacctttc atagaaggcg gcggtggaat cgaaatctcg tagcacgtgt cagtcctgct
     6421 cctcggccac gaagtgctta gccctcccac acataaccag agggcagcaa ttcacgaatc
     6481 ccaactgccg tcggctgtcc atcactgtcc ttcactatgg ctttgatccc aggatgcaga
     6541 tcgagaagca cctgtcggca ccgtccgcag gggctcaaga tgcccctgtt ctcatttccg
     6601 atcgcgacga tacaagtcag gttgccagct gccgcagcag cagcagtgcc cagcaccacg
     6661 agttctgcac aaggtccccc agtaaaatga tatacattga caccagtgaa gatgcggccg
     6721 tcgctagaga gagctgcgct ggcgacgctg tagtcttcag agatggggat gctgttgatt
     6781 gtagccgttg ctctttcaat gagggtggat tcttcttgag acaaaggctt ggccatggtt
     6841 tagttcctca ccttgtcgta ttatactatg ccgatatact atgccgatga ttaattgtca
     6901 acacgtgctg atcagatccg aaaatggata tacaagctcc cgggagcttt ttgcaaaagc
     6961 ctaggcctcc aaaaaagcct cctcactact tctggaatag ctcagaggca gaggcggcct
     7021 cggcctctgc ataaataaaa aaaattagtc agccatgggg cggagaatgg gcggaactgg
     7081 gcggagttag gggcgggatg ggcggagtta ggggcgggac tatggttgct gactaattga
     7141 gatgcatgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca cacctggttg
     7201 ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc
     7261 cacaccctaa ctgacacaca ttccacagaa ttcactgact cgctgcgctc ggtcgttcgg
     7321 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     7381 gataacgcag gaaagaacat gtaagtgatc tccaaaaaat aagtactttt tgactgtaaa
     7441 taaaattgta aggagtaaaa agtacttttt tttctaaaaa aatgtaatta agtaaaagta
     7501 aaagtattga tttttaattg tactcaagta aagtaaaaat ccccaaaaat aatacttaag
     7561 tacagtaatc aagtaaaatt actcaagtac tttacacctc tgcatgtgag caaaaggcca
     7621 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     7681 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     7741 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     7801 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     7861 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     7921 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     7981 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     8041 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     8101 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     8161 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     8221 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     8281 tgacactcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     8341 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     8401 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     8461 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     8521 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgggacccac gctcaccggc
     8581 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     8641 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     8701 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     8761 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     8821 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     8881 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     8941 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     9001 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     9061 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     9121 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     9181 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     9241 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     9301 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     9361 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga
     9421 cggatcggga gatcgatctc ccgatcccct agggtcgact ctcagtacaa tctgctctga
     9481 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
     9541 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
     9601 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgggtacc
     9661 ggcatatggt tcttgacaga ggtgtaaaaa gtactcaaaa attttactca agtgaaagta
     9721 caagtactta gggaaaattt tactcaatta aaagtaaaag tatctggcta gaatcttact
     9781 tgagtaaaag taaaaaagta ctccattaaa attgtacttg agtattaacg cgttgacatt
     9841 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
     9901 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     9961 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
    10021 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
    10081 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
    10141 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
    10201 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
    10261 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
    10321 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
    10381 gtaggcgtgt acggtgggag gtctatataa gc
//
