LOCUS       sequence-304697-       10382 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A-to-G base editor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10382)
  AUTHORS   Arbab M, Shen MW, Mok B, Wilson C, Matuszek Z, Cassa CA, Liu DR
  TITLE     Determinants of Base Editing Outcomes from Target Library Analysis 
            and Machine Learning.
  JOURNAL   Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 
            10.1016/j.cell.2020.05.037.
  PUBMED    32533916
REFERENCE   2  (bases 1 to 10382)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2020 
            Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037."
COMMENT     Sequence Label: p2T-CMV-ABEmaxCP1041-BlastR
FEATURES             Location/Qualifiers
     source          1..10382
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             116..136
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2369..4084
                     /codon_start=1
                     /product="N-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(N)
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE"
     CDS             5528..5548
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5557..5574
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5597..5614)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5603..5827
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5898..5914)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5898..5914)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5911..5933)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5922..5938
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5946..5952,5953..5970,5971..5976))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5991..6012
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    6129..6250
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6140..6159)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6194..6213
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(6408..6806)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(6825..6872)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(6920..7249)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6934..7069
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6988..7007)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     7234..7254
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     misc_feature    join(7373..7560,7561..7572)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     rep_origin      complement(7633..8221)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7713..7732)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8392..9183,9184..9252))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9015..9034
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9253..9357)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9436..9455)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(9647..9658,9659..9796)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     enhancer        9805..10184
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        join(10185..10382,1..6)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10339..10359
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 agagctggtt tagtgaaccg tcagatccgc tagagatccg cggccgctaa tacgactcac
       61 tatagggaga gccgccacca tgaaacggac agccgacgga agcgagttcg agtcaccaaa
      121 gaagaagcgg aaagtctctg aagtcgagtt tagccacgag tattggatga ggcacgcact
      181 gaccctggca aagcgagcat gggatgaaag agaagtcccc gtgggcgccg tgctggtgca
      241 caacaataga gtgatcggag agggatggaa caggccaatc ggccgccacg accctaccgc
      301 acacgcagag atcatggcac tgaggcaggg aggcctggtc atgcagaatt accgcctgat
      361 cgatgccacc ctgtatgtga cactggagcc atgcgtgatg tgcgcaggag caatgatcca
      421 cagcaggatc ggaagagtgg tgttcggagc acgggacgcc aagaccggcg cagcaggctc
      481 cctgatggat gtgctgcacc accccggcat gaaccaccgg gtggagatca cagagggaat
      541 cctggcagac gagtgcgccg ccctgctgag cgatttcttt agaatgcgga gacaggagat
      601 caaggcccag aagaaggcac agagctccac cgactctgga ggatctagcg gaggatcctc
      661 tggaagcgag acaccaggca caagcgagtc cgccacacca gagagctccg gcggctcctc
      721 cggaggatcc tctgaggtgg agttttccca cgagtactgg atgagacatg ccctgaccct
      781 ggccaagagg gcacgcgatg agagggaggt gcctgtggga gccgtgctgg tgctgaacaa
      841 tagagtgatc ggcgagggct ggaacagagc catcggcctg cacgacccaa cagcccatgc
      901 cgaaattatg gccctgagac agggcggcct ggtcatgcag aactacagac tgattgacgc
      961 caccctgtac gtgacattcg agccttgcgt gatgtgcgcc ggcgccatga tccactctag
     1021 gatcggccgc gtggtgtttg gcgtgaggaa cgcaaaaacc ggcgccgcag gctccctgat
     1081 ggacgtgctg cactaccccg gcatgaatca ccgcgtcgaa attaccgagg gaatcctggc
     1141 agatgaatgt gccgccctgc tgtgctattt ctttcggatg cctagacagg tgttcaatgc
     1201 tcagaagaag gcccagagct ccaccgactc cggaggatct agcggaggct cctctggctc
     1261 tgagacacct ggcacaagcg agagcgcaac acctgaaagc agcgggggca gcagcggggg
     1321 gtcaaacatc atgaactttt tcaagaccga gattaccctg gccaacggcg agatccggaa
     1381 gcggcctctg atcgagacaa acggcgaaac cggggagatc gtgtgggata agggccggga
     1441 ttttgccacc gtgcggaaag tgctgagcat gccccaagtg aatatcgtga aaaagaccga
     1501 ggtgcagaca ggcggcttca gcaaagagtc tatcctgccc aagaggaaca gcgataagct
     1561 gatcgccaga aagaaggact gggaccctaa gaagtacggc ggcttcgaca gccccaccgt
     1621 ggcctattct gtgctggtgg tggccaaagt ggaaaagggc aagtccaaga aactgaagag
     1681 tgtgaaagag ctgctgggga tcaccatcat ggaaagaagc agcttcgaga agaatcccat
     1741 cgactttctg gaagccaagg gctacaaaga agtgaaaaag gacctgatca tcaagctgcc
     1801 taagtactcc ctgttcgagc tggaaaacgg ccggaagaga atgctggcct ctgccggcga
     1861 actgcagaag ggaaacgaac tggccctgcc ctccaaatat gtgaacttcc tgtacctggc
     1921 cagccactat gagaagctga agggctcccc cgaggataat gagcagaaac agctgtttgt
     1981 ggaacagcac aagcactacc tggacgagat catcgagcag atcagcgagt tctccaagag
     2041 agtgatcctg gccgacgcta atctggacaa agtgctgtcc gcctacaaca agcaccggga
     2101 taagcccatc agagagcagg ccgagaatat catccacctg tttaccctga ccaatctggg
     2161 agcccctgcc gccttcaagt actttgacac caccatcgac cggaagaggt acaccagcac
     2221 caaagaggtg ctggacgcca ccctgatcca ccagagcatc accggcctgt acgagacacg
     2281 gatcgacctg tctcagctgg gaggcgacgg cggatcagga ggctctggcg gttcaggtgg
     2341 atcaggcggt agcggaggtt caggtggtga caagaagtac agcatcggcc tggccatcgg
     2401 caccaactct gtgggctggg ccgtgatcac cgacgagtac aaggtgccca gcaagaaatt
     2461 caaggtgctg ggcaacaccg accggcacag catcaagaag aacctgatcg gagccctgct
     2521 gttcgacagc ggcgaaacag ccgaggccac ccggctgaag agaaccgcca gaagaagata
     2581 caccagacgg aagaaccgga tctgctatct gcaagagatc ttcagcaacg agatggccaa
     2641 ggtggacgac agcttcttcc acagactgga agagtccttc ctggtggaag aggataagaa
     2701 gcacgagcgg caccccatct tcggcaacat cgtggacgag gtggcctacc acgagaagta
     2761 ccccaccatc taccacctga gaaagaaact ggtggacagc accgacaagg ccgacctgcg
     2821 gctgatctat ctggccctgg cccacatgat caagttccgg ggccacttcc tgatcgaggg
     2881 cgacctgaac cccgacaaca gcgacgtgga caagctgttc atccagctgg tgcagaccta
     2941 caaccagctg ttcgaggaaa accccatcaa cgccagcggc gtggacgcca aggccatcct
     3001 gtctgccaga ctgagcaaga gcagacggct ggaaaatctg atcgcccagc tgcccggcga
     3061 gaagaagaat ggcctgttcg gaaacctgat tgccctgagc ctgggcctga cccccaactt
     3121 caagagcaac ttcgacctgg ccgaggatgc caaactgcag ctgagcaagg acacctacga
     3181 cgacgacctg gacaacctgc tggcccagat cggcgaccag tacgccgacc tgtttctggc
     3241 cgccaagaac ctgtccgacg ccatcctgct gagcgacatc ctgagagtga acaccgagat
     3301 caccaaggcc cccctgagcg cctctatgat caagagatac gacgagcacc accaggacct
     3361 gaccctgctg aaagctctcg tgcggcagca gctgcctgag aagtacaaag agattttctt
     3421 cgaccagagc aagaacggct acgccggcta cattgacggc ggagccagcc aggaagagtt
     3481 ctacaagttc atcaagccca tcctggaaaa gatggacggc accgaggaac tgctcgtgaa
     3541 gctgaacaga gaggacctgc tgcggaagca gcggaccttc gacaacggca gcatccccca
     3601 ccagatccac ctgggagagc tgcacgccat tctgcggcgg caggaagatt tttacccatt
     3661 cctgaaggac aaccgggaaa agatcgagaa gatcctgacc ttccgcatcc cctactacgt
     3721 gggccctctg gccaggggaa acagcagatt cgcctggatg accagaaaga gcgaggaaac
     3781 catcaccccc tggaacttcg aggaagtggt ggacaagggc gcttccgccc agagcttcat
     3841 cgagcggatg accaacttcg ataagaacct gcccaacgag aaggtgctgc ccaagcacag
     3901 cctgctgtac gagtacttca ccgtgtataa cgagctgacc aaagtgaaat acgtgaccga
     3961 gggaatgaga aagcccgcct tcctgagcgg cgagcagaaa aaggccatcg tggacctgct
     4021 gttcaagacc aaccggaaag tgaccgtgaa gcagctgaaa gaggactact tcaagaaaat
     4081 cgagtgcttc gactccgtgg aaatctccgg cgtggaagat cggttcaacg cctccctggg
     4141 cacataccac gatctgctga aaattatcaa ggacaaggac ttcctggaca atgaggaaaa
     4201 cgaggacatt ctggaagata tcgtgctgac cctgacactg tttgaggaca gagagatgat
     4261 cgaggaacgg ctgaaaacct atgcccacct gttcgacgac aaagtgatga agcagctgaa
     4321 gcggcggaga tacaccggct ggggcaggct gagccggaag ctgatcaacg gcatccggga
     4381 caagcagtcc ggcaagacaa tcctggattt cctgaagtcc gacggcttcg ccaacagaaa
     4441 cttcatgcag ctgatccacg acgacagcct gacctttaaa gaggacatcc agaaagccca
     4501 ggtgtccggc cagggcgata gcctgcacga gcacattgcc aatctggccg gcagccccgc
     4561 cattaagaag ggcatcctgc agacagtgaa ggtggtggac gagctcgtga aagtgatggg
     4621 ccggcacaag cccgagaaca tcgtgatcga aatggccaga gagaaccaga ccacccagaa
     4681 gggacagaag aacagccgcg agagaatgaa gcggatcgaa gagggcatca aagagctggg
     4741 cagccagatc ctgaaagaac accccgtgga aaacacccag ctgcagaacg agaagctgta
     4801 cctgtactac ctgcagaatg ggcgggatat gtacgtggac caggaactgg acatcaaccg
     4861 gctgtccgac tacgatgtgg accatatcgt gcctcagagc tttctgaagg acgactccat
     4921 cgacaacaag gtgctgacca gaagcgacaa gaaccggggc aagagcgaca acgtgccctc
     4981 cgaagaggtc gtgaagaaga tgaagaacta ctggcggcag ctgctgaacg ccaagctgat
     5041 tacccagaga aagttcgaca atctgaccaa ggccgagaga ggcggcctga gcgaactgga
     5101 taaggccggc ttcatcaaga gacagctggt ggaaacccgg cagatcacaa agcacgtggc
     5161 acagatcctg gactcccgga tgaacactaa gtacgacgag aatgacaagc tgatccggga
     5221 agtgaaagtg atcaccctga agtccaagct ggtgtccgat ttccggaagg atttccagtt
     5281 ttacaaagtg cgcgagatca acaactacca ccacgcccac gacgcctacc tgaacgccgt
     5341 cgtgggaacc gccctgatca aaaagtaccc taagctggaa agcgagttcg tgtacggcga
     5401 ctacaaggtg tacgacgtgc ggaagatgat cgccaagagc gagcaggaaa tcggcaaggc
     5461 taccgccaag tacttcttct acagctctgg cggctcaaaa agaaccgccg acggcagcga
     5521 attcgagccc aagaagaaga ggaaagtcta accggtcatc atcaccatca ccattgagtt
     5581 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     5641 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     5701 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     5761 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     5821 tctatggctt ctgaggcgga aagaaccagc tggggctcga taccgtcgac ctctagctag
     5881 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     5941 ccacacaaca tacgagccgg aagcataaag tgtaaagcct agggtgccta atgagtgagc
     6001 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     6061 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctac
     6121 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     6181 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     6241 taaacaagtt ggggtgggcg aagaactcca gcatgagatc cccgcgctgg aggatcatcc
     6301 agccggcgtc ccggaaaacg attccgaagc ccaacctttc atagaaggcg gcggtggaat
     6361 cgaaatctcg tagcacgtgt cagtcctgct cctcggccac gaagtgctta gccctcccac
     6421 acataaccag agggcagcaa ttcacgaatc ccaactgccg tcggctgtcc atcactgtcc
     6481 ttcactatgg ctttgatccc aggatgcaga tcgagaagca cctgtcggca ccgtccgcag
     6541 gggctcaaga tgcccctgtt ctcatttccg atcgcgacga tacaagtcag gttgccagct
     6601 gccgcagcag cagcagtgcc cagcaccacg agttctgcac aaggtccccc agtaaaatga
     6661 tatacattga caccagtgaa gatgcggccg tcgctagaga gagctgcgct ggcgacgctg
     6721 tagtcttcag agatggggat gctgttgatt gtagccgttg ctctttcaat gagggtggat
     6781 tcttcttgag acaaaggctt ggccatggtt tagttcctca ccttgtcgta ttatactatg
     6841 ccgatatact atgccgatga ttaattgtca acacgtgctg atcagatccg aaaatggata
     6901 tacaagctcc cgggagcttt ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact
     6961 tctggaatag ctcagaggca gaggcggcct cggcctctgc ataaataaaa aaaattagtc
     7021 agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta
     7081 ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg
     7141 gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac
     7201 ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca ttccacagaa
     7261 ttcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     7321 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtaagtgatc
     7381 tccaaaaaat aagtactttt tgactgtaaa taaaattgta aggagtaaaa agtacttttt
     7441 tttctaaaaa aatgtaatta agtaaaagta aaagtattga tttttaattg tactcaagta
     7501 aagtaaaaat ccccaaaaat aatacttaag tacagtaatc aagtaaaatt actcaagtac
     7561 tttacacctc tgcatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     7621 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     7681 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     7741 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     7801 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     7861 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     7921 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     7981 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     8041 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     8101 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     8161 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8221 agaagatcct ttgatctttt ctacggggtc tgacactcag tggaacgaaa actcacgtta
     8281 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     8341 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     8401 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     8461 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     8521 aatgataccg cgggacccac gctcaccggc tccagattta tcagcaataa accagccagc
     8581 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     8641 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     8701 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     8761 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     8821 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     8881 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     8941 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     9001 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     9061 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     9121 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     9181 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     9241 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     9301 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     9361 atttccccga aaagtgccac ctgacgtcga cggatcggga gatcgatctc ccgatcccct
     9421 agggtcgact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc
     9481 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca
     9541 aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg
     9601 cgatgtacgg gccagatata cgcgggtacc ggcatatggt tcttgacaga ggtgtaaaaa
     9661 gtactcaaaa attttactca agtgaaagta caagtactta gggaaaattt tactcaatta
     9721 aaagtaaaag tatctggcta gaatcttact tgagtaaaag taaaaaagta ctccattaaa
     9781 attgtacttg agtattaacg cgttgacatt gattattgac tagttattaa tagtaatcaa
     9841 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     9901 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     9961 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
    10021 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
    10081 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
    10141 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
    10201 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
    10261 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
    10321 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
    10381 gc
//
