LOCUS       sequence-304811-         793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses ratiometric sensor of lysosomal lumenal pH.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Ponsford AH, Ryan TA, Raimondi A, Cocucci E, Wycislo SA, Frohlich F,
            Swan LE, Stagi M
  TITLE     Live imaging of intra-lysosome pH in cell lines and primary neuronal
            culture using a novel genetically encoded biosensor.
  JOURNAL   Autophagy. 2020 Jun 9:1-19. doi: 10.1080/15548627.2020.1771858.
  PUBMED    32515674
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Autophagy. 
            2020 Jun 9:1-19. doi: 10.1080/15548627.2020.1771858."
COMMENT     Sequence Label: pCAG R2pH-LAMP1-3xFLAG
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(229..247)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     497..516
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ccntggcggc ccccggcgcc cggcggccgc tgctcctgct gctgctggca ggccttgcac
       61 atggcgcctc agcagctagc ggtatggtga gcaagggcga ggaggataac atggccatca
      121 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg
      181 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
      241 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg
      301 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc
      361 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga
      421 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca
      481 acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg
      541 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga
      601 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc
      661 agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact
      721 acaccatcgt ggaacagtac gaacgcgccg aggggccgcc actccaccgg cggcatggac
      781 nagctgtaca ang
//
