LOCUS       sequence-30482-e         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Machacek M, Hodgson L, Welch C, Elliott H, Pertz O, Nalbant P, Abell
            A, Johnson GL, Hahn KM, Danuser G
  TITLE     Coordination of Rho GTPase activities during cell protrusion.
  JOURNAL   Nature. 2009 Sep 3. 461(7260):99-103.
  PUBMED    19693013
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Sep 3. 461(7260):99-103."
COMMENT     Sequence Label: pBabe-CyPet-Rac1
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    493..509
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(577..600)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 gccatgttat ggtagatgga aaaccggtga atctgggctt atgggataca gctggacaag
       61 aagattatga cagattacgc cccctatcct atccgcaaac agatgtgttc ttaatttgct
      121 tttcccttgt gagtcctgca tcatttgaaa atgtccgtgc aaagtggtat cctgaggtgc
      181 ggcaccactg tcccaacact cccatcatcc tagtgggaac taaacttgat cttagggatg
      241 ataaagacac gatcgagaaa ctgaaggaga agaagctgac tcccatcacc tatccgcagg
      301 gtctagccat ggctaaggag attggtgccg taaaatacct ggagtgctcg gcgctcacac
      361 agcgaggcct caagacagtg tttgacgaag cgatccgagc agtcctctgc ccgcctcccg
      421 tgaagaagag gaagagaaaa tgcctgctgt tgtaaatgaa ttaattcgtt aacgcatgcc
      481 tcgagtgtgg aattgtgagc ggataacaat ttgtcgaggg cccctgcagg tcaattctac
      541 cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg
      601 ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg
      661 cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc
      721 aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca
      781 cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt
      841 tggggcagcg gccaa
//
