LOCUS       sequence-30501-e         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Singla V, Hunkapiller J, Santos N, Seol AD, Norman AR, Wakenight P, 
            Skarnes WC, Reiter JF
  TITLE     Floxin, a resource for genetically engineering mouse ESCs.
  JOURNAL   Nat Methods. 2010 Jan . 7(1):50-2.
  PUBMED    19966808
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2010 Jan . 7(1):50-2."
COMMENT     Sequence Label: pFloxin-IRES-TAP
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             153..170
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             198..218
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             225..290
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    479..613
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(516..535)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     570..589
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    631..678
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(701..717)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(701..717)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(714..736)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    725..741
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt
       61 taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat
      121 gataatagga tcgcatgcgg cgcgccacta gtcatcacca tcaccatcac gattatgata
      181 ttccaactac tgctagcgag aatttgtatt ttcagggtga gctcgactac aaagaccatg
      241 acggtgatta taaagatcat gacatcgact acaaggatga cgatgacaag taggatatcg
      301 ctagcggatc caccacctac agcccagtgg ggtcgctcta gagcgacctc gagggggggc
      361 tagataagta atgatcataa tcagccatat cacatctgta gaggttttac ttgctttaaa
      421 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
      481 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
      541 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
      601 tcatgtctag atccgggggt accgcgtcga gaagttccta ttccgaagtt cctattctct
      661 agaaagtata ggaacttcgt cgacgcgaat tcgtaatcat gtcatagctg tttcctgtgt
      721 gaaattgtta tccgctcaca attccacaca
//
