LOCUS       sequence-305088-       10486 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express soluble His-tagged zebrafish Kitlgb in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10486)
  AUTHORS   Oltova J, Svoboda O, Machonova O, Svatonova P, Traver D, Kolar M, 
            Bartunek P
  TITLE     Zebrafish Kit ligands cooperate with erythropoietin to promote 
            erythroid cell expansion.
  JOURNAL   Blood Adv. 2020 Dec 8;4(23):5915-5924. doi: 
            10.1182/bloodadvances.2020001700.
  PUBMED    33259600
REFERENCE   2  (bases 1 to 10486)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1182/bloodadvances"; journalName: "Blood Adv"; date: "2020-12-8-
            8"; volume: "4"; issue: "23"; pages: "5915-5924"
COMMENT     Sequence Label: pAc-­His-­zfKitlgb
FEATURES             Location/Qualifiers
     source          1..10486
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     3170..3997
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             3170..3294
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(3332..3937)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     promoter        4001..4092
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     4017..4034
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     sig_peptide     4189..4248
                     /codon_start=1
                     /gene="Autographa californica gp64"
                     /product="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
                     /label=GP64 signal sequence
                     /translation="MVSAIVLYVLLAAAAHSAFA"
     CDS             4294..4311
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_recomb     5494..6199
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     3'UTR           5582..5954
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     CDS             complement(5611..6199)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     CDS             complement(6633..6650)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7867..7883)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7867..7883)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7880..7902)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7891..7907
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7915..7921,7922..7939,7940..7945))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7960..7981
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8098..8115)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8269..8857)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8349..8368)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9028..9819,9820..9888))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9651..9670
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9889..9993)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10061..10079
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10117..10139)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10239..10258
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10452..10474
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10466..10483
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10467..10483
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagcttggct cgtaaagcga gttgaaggat catatttagt tgcgtttatg agataagatt
       61 gaaagcacgt gtaaaatgtt tcccgcgcgt tggcacaact atttacaatg cggccaagtt
      121 ataaaagatt ctaatctgat atgttttaaa acacctttgc ggcccgagtt gtttgcgtac
      181 gtgactagcg aagaagatat gtggaccgca gaacagatag taaaacaaaa ccctagtatt
      241 ggagcaataa tcgatttaac caacacgtct aaatattatg atggtgtgca ttttttgcgg
      301 gcgggcctgt tatacaaaaa aattcaagta cctggccaga ctttgccgcc tgaaagcata
      361 gttcaagaat ttattgacac ggtaaaagaa tttacagaaa agtgtcccgg catgttggtg
      421 ggcgtgcact gcacacacgg tattaatcgc accggttaca tggtgtgcag atatttaatg
      481 cacaccctgg gtattgcgcc gcaggaagcc atagatagat tcgaaaaagc cagaggtcac
      541 aaaattgaaa gacaaaatta cgttcaagat ttattaattt aattaatatt atttgcattc
      601 tttaacaaat actttatcct attttcaaat tgttgcgctt cttccagcga accaaaacta
      661 tgcttcgctt gctccgttta gcttgtagcc gatcagtggc gttgttccaa tcgacggtag
      721 gattaggccg gatattctcc accacaatgt tggcaacgtt gatgttacgt ttatgctttt
      781 ggttttccac gtacgtcttt tggccggtaa tagccgtaaa cgtagtgccg tcgcgcgtca
      841 cgcacaacac cggatgtttg cgcttgtccg cggggtattg aaccgcgcga tccgacaaat
      901 ccaccacttt ggcaactaaa tcggtgacct gcgcgtcttt tttctgcatt atttcgtctt
      961 tcttttgcat ggtttcctgg aagccggtgt acatgcggtt tagatcagtc atgacgcgcg
     1021 tgacctgcaa atctttggcc tcgatctgct tgtccttgat ggcaacgatg cgttcaataa
     1081 actcttgttt tttaacaagt tcctcggttt tttgcgccac caccgcttgc agcgcgtttg
     1141 tgtgctcggt gaatgtcgca atcagcttag tcaccaactg tttgctctcc tcctcccgtt
     1201 gtttgatcgc gggatcgtac ttgccggtgc agagcacttg aggaattact tcttctaaaa
     1261 gccattcttg taattctatg gcgtaaggca atttggactt cataatcagc tgaatcacgc
     1321 cggatttagt aatgagcact gtatgcggct gcaaatacag cgggtcgccc cttttcacga
     1381 cgctgttaga ggtagggccc ccattttgga tggtctgctc aaataacgat ttgtatttat
     1441 tgtctacatg aacacgtata gctttatcac aaactgtata ttttaaactg ttagcgacgt
     1501 ccttggccac gaaccggacc tgttggtcgc gctctagcac gtaccgcagg ttgaacgtat
     1561 cttctccaaa tttaaattct ccaattttaa cgcgagccat tttgatacac gtgtgtcgat
     1621 tttgcaacaa ctattgtttt ttaacgcaaa ctaaacttat tgtggtaagc aataattaaa
     1681 tatgggggaa catgcgccgc tacaacactc gtcgttatga acgcagacgg cgccggtctc
     1741 ggcgcaagcg gctaaaacgt gttgcgcgtt caacgcggca aacatcgcaa aagccaatag
     1801 tacagttttg atttgcatat taacggcgat tttttaaatt atcttattta ataaatagtt
     1861 atgacgccta caactccccg cccgcgttga ctcgctgcac ctcgagcagt tcgttgacgc
     1921 cttcctccgt gtggccgaac acgtcgagcg ggtggtcgat gaccagcggc gtgccgcacg
     1981 cgacgcacaa gtatctgtac accgaatgat cgtcgggcga aggcacgtcg gcctccaagt
     2041 ggcaatattg gcaaattcga aaatatatac agttgggttg tttgcgcata tctatcgtgg
     2101 cgttgggcat gtacgtccga acgttgattt gcatgcaagc cgaaattaaa tcattgcgat
     2161 tagtgcgatt aaaacgttgt acatcctcgc ttttaatcat gccgtcgatt aaatcgcgca
     2221 atcgagtcaa gtgatcaaag tgtggaataa tgttttcttt gtattcccga gtcaagcgca
     2281 gcgcgtattt taacaaacta gccatcttgt aagttagttt catttaatgc aactttatcc
     2341 aataatatat tatgtatcgc acgtcaagaa ttaacaatgc gcccgttgtc gcatctcaac
     2401 acgactatga tagagatcaa ataaagcgcg aattaaatag cttgcgacgc aacgtgcacg
     2461 atctgtgcac gcgttccggc acgagctttg attgtaataa gtttttacga agcgatgaca
     2521 tgacccccgt agtgacaacg atcacgccca aaagaactgc cgactacaaa attaccgagt
     2581 atgtcggtga cgttaaaact attaagccat ccaatcgacc gttagtcgaa tcaggaccgc
     2641 tggtgcgaga agccgcgaag tatggcgaat gcatcgtata acgtgtggag tccgctcatt
     2701 agagcgtcat gtttagacaa gaaagctaca tatttaattg atcccgatga ttttattgat
     2761 aaattgaccc taactccata cacggtattc tacaatggcg gggttttggt caaaatttcc
     2821 ggactgcgat tgtacatgct gttaacggct ccgcccacta ttaatgaaat taaaaattcc
     2881 aattttaaaa aacgcagcaa gagaaacatt tgtatgaaag aatgcgtaga aggaaagaaa
     2941 aatgtcgtcg acatgctgaa caacaagatt aatatgcctc cgtgtataaa aaaaatattg
     3001 aacgatttga aagaaaacaa tgtaccgcgc ggcggtatgt acaggaagag gtttatacta
     3061 aactgttaca ttgcaaacgt ggtttcgtgt gccaagtgtg aaaaccgatg tttaatcaag
     3121 gctctgacgc atttctacaa ccacgactcc aagtgtgtgg gtgaagtcat gcatctttta
     3181 atcaaatccc aagatgtgta taaaccacca aactgccaaa aaatgaaaac tgtcgacaag
     3241 ctctgtccgt ttgctggcaa ctgcaagggt ctcaatccta tttgtaatta ttgaataata
     3301 aaacaattat aaatgtcaaa tttgtttttt attaacgata caaaccaaac gcaacaagaa
     3361 catttgtagt attatctata attgaaaacg cgtagttata atcgctgagg taatatttaa
     3421 aatcattttc aaatgattca cagttaattt gcgacaatat aattttattt tcacataaac
     3481 tagacgcctt gtcgtcttct tcttcgtatt ccttctcttt ttcatttttc tcctcataaa
     3541 aattaacata gttattatcg tatccatata tgtatctatc gtatagagta aattttttgt
     3601 tgtcataaat atatatgtct tttttaatgg ggtgtatagt accgctgcgc atagtttttc
     3661 tgtaatttac aacagtgcta ttttctggta gttcttcgga gtgtgttgct ttaattatta
     3721 aatttatata atcaatgaat ttgggatcgt cggttttgta caatatgttg ccggcatagt
     3781 acgcagcttc ttctagttca attacaccat tttttagcag caccggatta acataacttt
     3841 ccaaaatgtt gtacgaaccg ttaaacaaaa acagttcacc tcccttttct atactattgt
     3901 ctgcgagcag ttgtttgttg ttaaaaataa cagccattgt aatgagacgc acaaactaat
     3961 atcacaaact ggaaatgtct atcaatatat agttgctgat atcatggaga taattaaaat
     4021 gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt tgtaataaaa
     4081 aaacctataa atattccgga ttattcatac cgtcccacca tcgggcgcgg atctatgcta
     4141 ctagtaaatc agtcacacca aggcttcaat aaggaacaca caagcaagat ggtaagcgct
     4201 attgttttat atgtgctttt ggcggcggcg gcgcattctg cctttgcggc ggatcttgga
     4261 tcccgggcca tgggaattct acatatggct agccaccatc accatcacca tggtggatcc
     4321 gggagccctt taacagatga tgtggccacg ttagataccc tgagtgagaa tattcccagt
     4381 gactacagaa tacccatcaa gttcatcaca aaggatgtgg gtggagcttg ctggctgcat
     4441 ttgaacctgt atccagtcga gagcagttta aaaaaattgg ccatcaagtt tggcaaccag
     4501 tccaccaata aggccaacat caccattttc ataacaatgc tgcaggattt tcgtttcact
     4561 ctgaacagtg acgacctgga ggacagaatg caagccttca agtgccacta cagaagggaa
     4621 aagtggccga ctaggcgctt ctttagctat gttaaatccg ttcttacagt ggccggctcc
     4681 acatatggtg atattcctcc atgcacacct cctccttgcc agaccctcgc agctcctcct
     4741 ttcacaccag gtcaaagcag acagcagaat ggcatgaact ctgcggttca ttaaggatcc
     4801 acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga
     4861 gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa
     4921 attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat
     4981 ggacgatgaa ttccggagcg gccgctgcag atctgatcct ttcctgggac ccggcaagaa
     5041 ccaaaaactc actctcttca aggaaatccg taatgttaaa cccgacacga tgaagcttgt
     5101 cgttggatgg aaaggaaaag agttctacag ggaaacttgg acccgcttca tggaagacag
     5161 cttccccatt gttaacgacc aagaagtgat ggatgttttc cttgttgtca acatgcgtcc
     5221 cactagaccc aaccgttgtt acaaattcct ggcccaacac gctctgcgtt gcgaccccga
     5281 ctatgtacct catgacgtga ttaggatcgt cgagccttca tgggtgggca gcaacaacga
     5341 gtaccgcatc agcctggcta agaagggcgg cggctgccca ataatgaacc ttcactctga
     5401 gtacaccaac tcgttcgaac agttcatcga tcgtgtcatc tgggagaact tctacaagcc
     5461 catcgtttac atcggtaccg actctgctga agaggaggaa attctccttg aagtttccct
     5521 ggtgttcaaa gtaaaggagt ttgcaccaga cgcacctctg ttcactggtc cggcgtatta
     5581 aaacacgata cattgttatt agtacattta ttaagcgcta gattctgtgc gttgttgatt
     5641 tacagacaat tgttgtacgt attttaataa ttcattaaat ttataatctt tagggtggta
     5701 tgttagagcg aaaatcaaat gattttcagc gtctttatat ctgaatttaa atattaaatc
     5761 ctcaatagat ttgtaaaata ggtttcgatt agtttcaaac aagggttgtt tttccgaacc
     5821 gatggctgga ctatctaatg gattttcgct caacgccaca aaacttgcca aatcttgtag
     5881 cagcaatcta gctttgtcga tattcgtttg tgttttgttt tgtaataaag gttcgacgtc
     5941 gttcaaaata ttatgcgctt ttgtatttct ttcatcactg tcgttagtgt acaattgact
     6001 cgacgtaaac acgttaaata aagcttggac atatttaaca tcgggcgtgt tagctttatt
     6061 aggccgatta tcgtcgtcgt cccaaccctc gtcgttagaa gttgcttccg aagacgattt
     6121 tgccatagcc acacgacgcc tattaattgt gtcggctaac acgtccgcga tcaaatttgt
     6181 agttgagctt tttggaatta tttctgattg cgggcgtttt tgggcgggtt tcaatctaac
     6241 tgtgcccgat tttaattcag acaacacgtt agaaagcgat ggtgcaggcg gtggtaacat
     6301 ttcagacggc aaatctacta atggcggcgg tggtggagct gatgataaat ctaccatcgg
     6361 tggaggcgca ggcggggctg gcggcggagg cggaggcgga ggtggtggcg gtgatgcaga
     6421 cggcggttta ggctcaaatg tctctttagg caacacagtc ggcacctcaa ctattgtact
     6481 ggtttcgggc gccgtttttg gtttgaccgg tctgagacga gtgcgatttt tttcgtttct
     6541 aatagcttcc aacaattgtt gtctgtcgtc taaaggtgca gcgggttgag gttccgtcgg
     6601 cattggtgga gcgggcggca attcagacat cgatggtggt ggtggtggtg gaggcgctgg
     6661 aatgttaggc acgggagaag gtggtggcgg cggtgccgcc ggtataattt gttctggttt
     6721 agtttgttcg cgcacgattg tgggcaccgg cgcaggcgcc gctggctgca caacggaagg
     6781 tcgtctgctt cgaggcagcg cttggggtgg tggcaattca atattataat tggaatacaa
     6841 atcgtaaaaa tctgctataa gcattgtaat ttcgctatcg tttaccgtgc cgatatttaa
     6901 caaccgctca atgtaagcaa ttgtattgta aagagattgt ctcaagctcg cacgccgata
     6961 acaagccttt tcatttttac tacagcattg tagtggcgag acacttcgct gtcgtcgacg
     7021 tacatgtatg ctttgttgtc aaaaacgtcg ttggcaagct ttaaaatatt taaaagaaca
     7081 tctctgttca gcaccactgt gttgtcgtaa atgttgtttt tgataatttg cgcttccgca
     7141 gtatcgacac gttcaaaaaa ttgatgcgca tcaattttgt tgttcctatt attgaataaa
     7201 taagattgta cagattcata tctacgattc gtcatggcca ccacaaatgc tacgctgcaa
     7261 acgctggtac aattttacga aaactgcaaa aacgtcaaaa ctcggtataa aataatcaac
     7321 gggcgctttg gcaaaatatc tattttatcg cacaagccca ctagcaaatt gtatttgcag
     7381 aaaacaattt cggcgcacaa ttttaacgct gacgaaataa aagttcacca gttaatgagc
     7441 gaccacccaa attttataaa aatctatttt aatcacggtt ccatcaacaa ccaagtgatc
     7501 gtgatggact acattgactg tcccgattta tttgaaacac tacaaattaa aggcgagctt
     7561 tcgtaccaac ttgttagcaa tattattaga cagctgtgtg aagcgctcaa cgatttgcac
     7621 aagcacaatt tcatacacaa cgacataaaa ctcgaaaatg tcttatattt cgaagcactt
     7681 gatcgcgtgt atgtttgcga ttacggattg tgcaaacacg aaaactcact tagcgtgcac
     7741 gacggcacgt tggagtattt tagtccggaa aaaattcgac accacaacta tgcacgttcg
     7801 tttgactggt acgccgtcgg cgtgttaaca tacaagttgc taaccggcgg ccaattcgta
     7861 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     7921 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     7981 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     8041 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     8101 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     8161 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     8221 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     8281 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     8341 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     8401 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     8461 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     8521 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     8581 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     8641 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     8701 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     8761 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     8821 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     8881 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     8941 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     9001 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     9061 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     9121 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     9181 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     9241 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     9301 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     9361 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     9421 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     9481 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     9541 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     9601 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     9661 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     9721 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     9781 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     9841 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     9901 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     9961 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
    10021 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
    10081 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
    10141 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
    10201 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
    10261 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
    10321 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
    10381 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
    10441 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgcc
//
