LOCUS       sequence-30511-e         755 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Singla V, Romaguera-Ros M, Garcia-Verdugo JM, Reiter JF
  TITLE     Ofd1, a human disease gene, regulates the length and distal 
            structure of centrioles.
  JOURNAL   Dev Cell. 2010 Mar 16. 18(3):410-24.
  PUBMED    20230748
REFERENCE   2  (bases 1 to 755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2010 Mar 16. 18(3):410-24."
COMMENT     Sequence Label: pFloxin-IRES-Ofd1-Myc-G139S
FEATURES             Location/Qualifiers
     source          1..755
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(31..78)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     polyA_signal    96..230
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(120..139)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     174..193
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(574..603)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gctatgacat gattacgaat tcgcgtcgac gaagttccta tactttctag agaataggaa
       61 cttcggaata ggaacttctc gacgcggtac ccccggatct agacatgata agatacattg
      121 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
      181 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
      241 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt
      301 aaaacctcta cagatgtgat atggctgatt atgatcatta cttatctagc cccccctcga
      361 ggtcgctcta gagcgacccc actgggctgt aggtggtgga tccgctagcg atctcacatt
      421 gccaaaagac ggcaatatgg tggaaaataa catatagaca aacgcacacc ggccttattc
      481 caagcggctt cggccagtaa cgttaggggg gggggaggga gaggggcgga tccactagta
      541 acggccgcca gtgtgctgga attcgccctt ttacaagtcc tcttcagaaa tgagcttttg
      601 ctcccacatg tcatctggtt cttcatgaga aaatcctgga gaactttcat ccttgtcact
      661 aggcatcatc atgtccacta gtgagcattc tttgcccgat tttttggagc tcttatctgc
      721 atttgattct tcctgtctct gctggataat tttca
//
