LOCUS       sequence-305259-         767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of CDK10 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 767)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-CDK10_IDG-K
FEATURES             Location/Qualifiers
     source          1..767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             95..136
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    137..161
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tgataaacag ggagccctgc tgctggaaca ggacggagtt atcaaaccct ggatgggcgg
       61 agaaatcagt ctgcggtctg ccgaaaaggc tagcggcaag cccatcccca accccctgct
      121 gggcctggac agcaccacaa ctttgtacaa aaaagcaggc tccaccatgg acaaggagaa
      181 ggatggcatc cccatcagca gcttgcggga gatcacgctg ctgctccgcc tgcgtcatcc
      241 gaacatcgtg gagctgaagg aggtggttgt ggggaaccac ctggagagca tcttcctggt
      301 gatgggttac tgtgagcagg acctggccag cctcctggag aatatgccaa cacccttctc
      361 ggaggctcag gtcaagtgca tcgtgctgca ggtgctccgg ggcctccagt atctgcacag
      421 gaacttcatt atccacaggg acctgaaggt ttccaacttg ctcatgaccg acaagggttg
      481 tgtgaagaca gcggatttcg gcctggcccg ggcctatggt gtcccagtaa agccaatgac
      541 ccccaaggtg gtcactctct ggtaccgagc ccctgaactg ctgttgggaa ccaccacgca
      601 gaccaccagc atcgacatgt gggctgtggg ctgcatactg gccgagctgc tggcgcacag
      661 gcctcttctc cccggcactt ccgagatcca ccagatcgac ttgatcgtgc agctgctggg
      721 cacgcccagt gagaacatct ggccgggctt ttccaagctg ccactgg
//
