LOCUS       sequence-305296-       18497 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express doxycycline-inducible full length of human XIST cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18497)
  AUTHORS   Jiang J, Jing Y, Cost GJ, Chiang JC, Kolpa HJ, Cotton AM, Carone DM,
            Carone BR, Shivak DA, Guschin DY, Pearl JR, Rebar EJ, Byron M, 
            Gregory PD, Brown CJ, Urnov FD, Hall LL, Lawrence JB
  TITLE     Translating dosage compensation to trisomy 21.
  JOURNAL   Nature. 2013 Aug 15;500(7462):296-300. doi: 10.1038/nature12394. 
            Epub 2013 Jul 17.
  PUBMED    23863942
REFERENCE   2  (bases 1 to 18497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature12394"; journalName: "Nature"; date: "2013-08-15- 
            15"; volume: "500"; issue: "7462"; pages: "296-300"
COMMENT     Sequence Label: DYRK1A/pTRE3G-FL-hXIST
FEATURES             Location/Qualifiers
     source          1..18497
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4..382
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    12..30
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    48..66
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    84..102
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    120..138
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    156..174
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    192..210
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    228..246
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     291..315
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          14445..14510
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             14640..14660
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    15085..15206
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(15122..15141)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     15176..15195
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(15292..15309)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(16155..16743)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(16235..16254)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(16914..17705,17706..17774))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     17537..17556
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(17775..17879)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     17947..17965
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctcgagttta ctccctatca gtgatagaga acgtatgaag agtttactcc ctatcagtga
       61 tagagaacgt atgcagactt tactccctat cagtgataga gaacgtataa ggagtttact
      121 ccctatcagt gatagagaac gtatgaccag tttactccct atcagtgata gagaacgtat
      181 ctacagttta ctccctatca gtgatagaga acgtatatcc agtttactcc ctatcagtga
      241 tagagaacgt ataagcttta ggcgtgtacg gtgggcgcct ataaaagcag agctcgttta
      301 gtgaaccgtc agatcgcctg gagcaattcc acaacacttt tgtcttatac caactttccg
      361 taccacttcc taccctcgta aagtcgacac cggggcccag atctggtacc gagctcggat
      421 ccactagtcc agtgtggtgg aattctgcag atctagaaca ttttctagtc ccccaacacc
      481 ctttatggcg tatttcttta aaaaaatcac ctaaattcca taaaatattt ttttaaattc
      541 tatactttct cctagtgtct tcttgacacg tcctccatat ttttttaaag aaagtatttg
      601 gaatattttg aggcaatttt taatatttaa ggaatttttc tttggaatca tttttggttg
      661 acatctctgt tttttgtgga tcagtttttt actcttccac tctcttttct atattttgcc
      721 catcggggct gcggatacct ggttttatta ttttttcttt gcccaacggg gccgtggata
      781 cctgcctttt aattcttttt tattcgccca tcggggccgc ggatacctgc tttttatttt
      841 tttttcctta gcccatcggg gtatcggata cctgctgatt cccttcccct ctgaaccccc
      901 aacactctgg cccatcgggg tgacggatat ctgcttttta aaaattttct ttttttggcc
      961 catcggggct tcggatacct gctttttttt tttttatttt tccttgccca tcggggcctc
     1021 ggatacctgc tttaattttt gtttttctgg cccatcgggg ccgcggatac ctgctttgat
     1081 tttttttttt catcgcccat cggtgctttt tatggatgaa aaaatgttgg ttttgtgggt
     1141 tgttgcactc tctggaatat ctacactttt ttttgctgct gatcatttgg tggtgtgtga
     1201 gtgtacctac cgctttggca gagaatgact ctgcagttaa gctaagggcg tgttcagatt
     1261 gtggaggaaa agtggccgcc attttagact tgccgcataa ctcggcttag ggctagtcgt
     1321 ttgtgctaag ttaaactagg gaggcaagat ggatgatagc aggtcaggca gaggaagtca
     1381 tgtgcattgc atgagctaaa cctatctgaa tgaattgatt tggggcttgt taggagcttt
     1441 gcgtgattgt tgtatcggga ggcagtaaga atcatctttt atcagtacaa gggactagtt
     1501 aaaaatggaa ggttaggaaa gactaaggtg cagggcttaa aatggcgatt ttgacattgc
     1561 ggcattgctc agcatggcgg gctgtgcttt gttaggttgt ccaaaatggc ggatccagtt
     1621 ctgtcgcagt gttcaagtgg cgggaaggcc acatcatgat gggcgaggct ttgttaagtg
     1681 gttagcatgg tggtggacat gtgcggtcac acaggaaaag atggcggctg aaggtcttgc
     1741 cgcagtgtaa aacatggcgg gcctctttgt ctttgctgtg tgcttttcgt gttgggtttt
     1801 gccgcaggga caatatggca ggcgttgtca tatgtatatc atggcttttg tcacgtggac
     1861 atcatggcgg gcttgccgca ttgttaaaga tggcgggttt tgccgcctag tgccacgcag
     1921 agcgggagaa aaggtgggat ggacagtgct ggattgctgc ataacccaac caattagaaa
     1981 tgggggtgga attgatcaca gccaattaga gcagaagatg gaattagact gatgacacac
     2041 tgtccagcta ctcagcgaag acctgggtga attagcatgg cacttcgcag ctgtctttag
     2101 ccagtcagga gaaagaagtg gaggggccac gtgtatgtct cccagtgggc ggtacaccag
     2161 gtgttttcaa ggtcttttca aggacattta gcctttccac ctctgtcccc tcttatttgt
     2221 cccctcctgt ccagtgctgc ctcttgcagt gctggatatc tggctgtgtg gtctgaacct
     2281 ccctccattc ctctgtattg gtgcctcacc taaggctaag tatacctccc ccccaccccc
     2341 caaccccccc aactccccac cccccccacc tccccacccc cctacccccc taccccccta
     2401 cccccctctg gtctgccctg cactgcactg ttgccatggg cagtgctcca ggcctgcttg
     2461 gtgtggacat ggtggtgagc cgtggcaagg accagaatgg atcacagatg atcgttggcc
     2521 aacaggtggc agaagaggaa ttcctgcctt cctcaagagg aacacctacc ccttggctaa
     2581 tgctggggtc ggattttgat ttatatttat cttttggatg tcagtcatac agtctgattt
     2641 tgtggtttgc tagtgtttga atttaagtct taagtgacta ttatagaaat gtattaagag
     2701 gctttatttg tagaattcac tttaattaca tttaatgagt ttttgttttg agttccttaa
     2761 aattccttaa agtttttagc ttctcattac aaattcctta accttttttt ggcagtagat
     2821 agtcaaagtc aaatcatttc taatgtttta aaaatgtgct ggtcattttc tttgaaattg
     2881 acttaactat tttcctttga agagtctgta gcacagaaac agtaaaaaat ttaacttcat
     2941 gacctaatgt aaaaaagagt gtttgaaggt ttacacaggt ccaggccttg ctttgttccc
     3001 atccttgatg ctgcactaat tgactaatca cctacttatc agacaggaaa cttgaattgc
     3061 tgtggtctgg tgtcctctat tcagacttat tatattggag tatttcaatt tttcgttgta
     3121 tcctgcctgc ctagcatcca gttcctcccc agccctgctc ccagcaaacc cctagtctag
     3181 ccccagccct actcccaccc cgccccagcc ctgccccagc cccagtcccc taacccccca
     3241 gccctagccc cagtcccagt cctagttcct cagtcccgcc cagcttctct cgaaagtcac
     3301 tctaattttc attgattcag tgctcaaaat aagttgtcca ttgcttatcc tattatactg
     3361 ggatattccg tttacccttg gcattgctga tcttcagtac tgactccttg accattttca
     3421 gttaatgcat acaatcccat ttgtctgtga tctcaggaca aagaatttcc ttactcggta
     3481 cgttgaagtt agggaatgtc aattgagagc tttctatcag agcattattg cccacaattt
     3541 gagttactta tcattttctc gatcccctgc ccttaaagga gaaaccattt ctctgtcatt
     3601 gcttctgtag tcacagtccc aattttgagt agtgatcttt tcttgtgtac tgtgttggcc
     3661 acctaaaact ctttgcattg agtaaaattc taattgccaa taatcctacc cattggatta
     3721 gacagcactc tgaaccccat ttgcattcag cagggggtcg cagacaaccc gtcttttgtt
     3781 ggacagttaa aatgctcagt cccaattgtc atagctttgc ctattaaaca aagacaccct
     3841 actgcgcttt ttgctgtgct tctggagaat cctgctgttc ttggacaatt aaagaacaaa
     3901 gtagtaattg ctaattgtct cacccattaa tcatgaagac taccagtcgc ccttgcattt
     3961 gccttgaggc agcgctgact acctgagatt taagagtttc ttaaattatt gagtaaaatc
     4021 ccaattatcc atagttctgt tagttacact atggcctttg caaacatctt tgcataacag
     4081 cagtgggact gactcattct tagagcccct tcccttggaa tattaatgga tacaatagta
     4141 attattcatg gttctgcgta acagagaaga cccacttatg tgtatgcctt tatcattgct
     4201 cctagatagt gtgaactacc taccaccttg cattaatatg taaaacacta attgcccata
     4261 gtcccactca ttagtctagg atgtcctctt tgccattgct gctgagttct gactacccaa
     4321 gtttccttct cttaaacagt tgatatgcat aattgcatat attcatggtt ctgtgcaata
     4381 aaaatggatt ctcaccccat cccaccttct gtgggatgtt gctaacgagt gcagattatt
     4441 caataacagc tcttgaacag ttaatttgca cagttgcaat tgtccagagt cctgtccatt
     4501 agaaagggac tctgtatcct atttgcacgc tacaatgtgg gctgatcacc caaggactct
     4561 tcttgtgcat tgatgttcat aattgtattt gtccacgatc ttgtgcacta acccttccac
     4621 tccctttgta ttccagcagg ggacccttac tactcaagac ctctgtacta ggacagttta
     4681 tgtgcacaat cctaattgat tagaactgag tcttttatat caaggtccct gcatcatctt
     4741 tgctttacat caagagggtg ctggttacct aatgcccctc ctccagaaat tattgatgtg
     4801 caaaatgcaa tttccctatc tgctgttagt ctggggtctc atcccctcat attccttttg
     4861 tcttacagca gggggtactt gggactgtta atacgcataa ttgcaattat ggtcttttcc
     4921 attaaattaa gatcccaact gctcacaccc tcttagcatt acagtagagg gtgctaatca
     4981 caaggacatt tcttttgtac tgttaatgtg ctacttgcat ttgtccctct tcctgtgcac
     5041 taaagacccc actcacttcc ctagtgttca gcagtggatg acctctagtc aagacctttg
     5101 cactaggata gttaatgtga accatggcaa ctgatcacaa caatgtcttt cagatcagat
     5161 ccattttatc ctccttgttt tacagcaagg gatattaatt acctatgtta cctttccctg
     5221 ggactatgaa tgtgcaaaat tccaatgttc atggtctctc cctttaaacc tatattctac
     5281 cccttttaca ttatagaaag ggatgctgga aacccagagt ccttctcttg ggactcttaa
     5341 tgtgtatttc taattatcca tgactcttaa tgtgcatatt ttcaattgcc taattgattt
     5401 caattgtcta agacatttca aatgtctaat tgattagaac tgagtctttt atatcaagct
     5461 aatatctagc ttttatatca agctaatatc ttgacttccc agcatcatag aagggggtac
     5521 tgatttccta aagtctttct tgaatttcta ttatgcaaaa ttgccctgag gccgggtgtg
     5581 gtggctcaca cctgtaatcc cagcactttg ggaggctgag gtgggaagat cccttactgc
     5641 caggagtttg agaccagcct ggccaacatt aaaaaaaaaa aaagtaagac aattgccctg
     5701 gaatcccatc cccctcacac ctccttggca aagcagcagg agtgctaact agctagtgct
     5761 tcttctctta tactgcttaa atgcgcataa ttagcagtag ttgatgtgcc cctatgttag
     5821 agtagaatcc cgcttccttg ctccatttgc attactgcag gagcttctaa ctagcctgaa
     5881 ttcactctct tggactgtta atgtgcatac ttatatttgc tgctgtactt ttttaccatg
     5941 taaggacccc acccactgta tttacatccc agctggaagt acctactact taagaccctt
     6001 agactagtaa agttagcgtg cataatctta ggtgttatat acacattttc agttgcatac
     6061 agttgtgcct tttatcagga ctcctgtact tatcaaagca gagagtgcta atcaatatta
     6121 agcccttctc ttcgaactgt agatggcatg taattgcagt tgtcaatggt ccttcaatta
     6181 gacttgggtt tctgacctat cacaccctct ttgctttatt gcatggggta ctattcactt
     6241 aaggcccctt tctcaaactg ttaatgtgcc taatgacaat tacatcagta tccttccttt
     6301 tgaaggacag catggttggt gacacctaag gccccatttc ttggcctccc aatatgtgtg
     6361 attgtatttg tcgaggttgc tatgcactag agaaggaaag tgctcccctc atccccactt
     6421 ttcccttcca gcaggaagtg cccaccccat aagacccttt tatttggaga gtctaggtgc
     6481 acaattgtaa gtgaccacaa gcatgcatct tggacattta tgtgcgtaat cgcacactgc
     6541 tcattccatg tgaataaggt cctactctcc gacccctttt gcaatacaga agggttgctg
     6601 ataacgcagt ccccttttct tggcatgttg tgtgtgatta taatcgtctg ggatcctatg
     6661 cactagaaaa ggagggtcct ctccacatac ctcagtctca cctttccctt ccagcaggga
     6721 gtgcccactc cataagactc tcacatttgg acagtcaagg tgcgtaattg ttaagtgaac
     6781 acaaccatgc accttagaca tggatttgca taactacaca cagctcaacc tatctgaata
     6841 aaatcctact ctcagacccc ttttgcagta cagcaggggt gctgatcacc aaggcccttt
     6901 ttcctggcct ggtatgcgtg tgattatgtt tgtcccggtt cctgtgtatt agacatggaa
     6961 gcctcccctg ccacactcca cccccaatct tcccttccct tccggcaggg agtgccctct
     7021 ccataagacg cttacgtttg gacaatcaag gtgcacagtt gtaagtgacc acaggcatac
     7081 accttggaca ttaatgtgca taaccacttt gcccattcca tctgaataag gtcctactct
     7141 cagacccctt ttgcagtaca gcaggggtgc tgatcaccaa ggcccctttt cttggcctgt
     7201 tatgtgcgtg attatatttg tctgggttcc tgtgtattag acaaggaagc cttccccccg
     7261 cccccacccc cactcccagt cttcctttcc cttccagcag ggagtgcccc ctccataaga
     7321 tcattacatt tggacaatca aggtgcacaa ttataagtga ccacagccat gcaccttgga
     7381 cattattgga cattaatgtg cgtaactgca catggcccat cccatctgaa taaggtccta
     7441 ctctcagatg ccctttgcag tacagcaggg gtactgaatc accaaggccc tttttcttgg
     7501 cctgttatgt gtgtgattat atttatccca gtttctgtgt aatagacatg aaagcctccc
     7561 ctgccacacc ccacctccaa tcttcctttc ccttccacca gggagtgtcc actccatata
     7621 cccttacatt tggacaatca aggtgcacaa ttgtaagtga gcataggcac tcaccttgga
     7681 catgaatgtg cataactgca catggcccat cccatctgaa taaggtccca ctctcagacc
     7741 cttttcgcag tacagcaggg gtgctgatca ccaaggcccc ttttcctggc ctgttatgtg
     7801 tgtgattata tttgttccag ttcctgtgta atagacatgg aagcctcccc tgccacactc
     7861 cacccccaat cttcctttcc cttctggcag gaagtacccg ctccataaga cccttacatt
     7921 tggacagtca aggtgcacaa ttgtatgtga ccacaaccat gcaccttgga cataaatgtg
     7981 tgtaactgca catggcccat cccatctgaa taaggtccta ctctcagacc ccttttgcag
     8041 tacagtaggt gtgctgataa ccaaggcccc tcttcctggc ctgttaacgt atgtgattat
     8101 atttgtctgg gttccagtgt ataagacatg gaagcctccc ctgccccacc ccaccctcaa
     8161 tcttcctttc ccttctggca gggagtgcca gctccataag aaccttacat ttggacagtc
     8221 aaggtgcaca attctaagtg accgcagcca tgcaccttgg tcaataatgt gtgtaactgc
     8281 acacggccta tctcatctga ataaggcctt actctcagac cccttttgca gtacagcagg
     8341 ggtgctgata accaaggccc attttcctgg cctgttatgt gtgtgattat atttgtccag
     8401 gtttctgtgt actagacaag gaagcctcct ctgccccatc ccatctacgc ataatctttc
     8461 ttttcctccc agcagggagt gctcactcca taagaccctt acatttggac aatcaaggtg
     8521 cacaattgta agtgaccaca accatgcatc ttggaaattt atgtgcataa ctgcacatgg
     8581 cttatcctat ttgaataaag tcctactctc agaccccctt tgcagtatag ctggggtgct
     8641 gatcactgag gcctctttgc ttggcttgtc tatattcttg tgtactagat aagggcacct
     8701 tctcatggac tccctttgct tttcaacaag gagtacccac tactttttaa gattcttata
     8761 tttgtccaaa gtacatggtt ttaattgacc acaacaatgt cccttggaca ttaatgtatg
     8821 taatcaccac atggttcatc ctaattaaac aaagttctac cttctcaccc tccatttgca
     8881 gtataccagg gttgctgacc ccctaagtcc ccttttcttg gcttgttgac atgcataatt
     8941 gcatttatgt tggttcttgt gccctagaca aggatgcccc acctcttttc aatagtgggt
     9001 gcccactcct tatgatcttt acatttgaac agttaatgtg aataattgca gttgtccaca
     9061 accctatcac ttctaggacc attatacctc ttttgcatta ctgtggggta tactgtttcc
     9121 ctccaaggcc ccttctggtg gactatcaac atataattga aattttcttt tgtctttgtc
     9181 agtagattaa ggtcataccc catcaccttt cctttgtagt acaacagggt gtcctgatca
     9241 accaaagtcc tgttgttttg gactgttaat atgtgcaatt acatttgctc ctgatctgtg
     9301 cactagataa ggatcctacc tactttctta gtgtttttag caggtagtgc ccactactca
     9361 agactgtcac ttggaatgtt catgtgcaca aactcaattc tctaagcatg ttcctgtacc
     9421 acctttgctt tagagcaggg ggatgatatt cactaagtgc cccttctttt ggacttaata
     9481 tgcattaatg caattgtcca cctcttcttt tagactaaga gttgatctcc acatattccc
     9541 cttgcatcag gggcatgtta attatgaatg aacccttttc ttttaatatt aatgtcataa
     9601 ttgtatttgt ggacctgtgt aggagaaaaa gaccctatgt tcctcccatt accctttgga
     9661 ttgctgctga gaagtgttaa ctactcataa tctcagctct tggacaatta atagcattaa
     9721 taacaattat caagggcact gatcattaga taagactcct gcttcctcgt tgcttacatc
     9781 gggggtactg acccactaag gccccttgta ctgttaatgt gaatatttgc aattatatat
     9841 gtctccttct ggtagagtgg gatattatgc cctagtatcc cctttgcatt actgcagggg
     9901 ctgctgacta ctcaaaactt ctcctgggac tgttaatagg cacaatggca gttatcaatg
     9961 gttttctccc tccctgacct tgttaagcaa gcgccccacc ccacccttag tttcccatgg
    10021 cataataaag tataagcatt ggagtattcc atgcacttgt ctatcaaaca gtggtccata
    10081 ctcccaaccc ttttgcattg cgccagtgtg taaaatcaca ggtagccatg gtgtcatgct
    10141 ttatatacga agtcttccct ctctctgccc cttgtgtgcc cttggcccct ttttacagac
    10201 tattgctcac aatctcaggt gtccatattt gcagctatta ggtaagattg tgctgtctcc
    10261 ctcttccctt ccctctgccc tgcccctttt gcctctttgc tgggtaatgt tgaccggaca
    10321 aggccctttc tcttggactt aaacaattct cagttgcact ttccttggtc ccacccatta
    10381 tacatgaacc cctctacttc ctttcgcatt gcttctgagt atgctgacta cccaaagccc
    10441 cttctgtgtt attaataaac acagtactga ttgtcccatt tttcagccca tcagtccaag
    10501 atctccctac cactttggtg tgttggtgca gtgttgacta tgaaaagcag gcctgaacta
    10561 ggtggataag ccttcactca ttttctttca tttattaatg atcctagttt caattattgt
    10621 cagattctgg ggacaagaac cattcttgcc cacctgtgtt actgctttac tgtgcaaaat
    10681 actgaaggca agtcagaccc agggagctgg attgccatcc tttattttgt gtttccagtg
    10741 tacactataa aattgtctcc ccaggaagga aggttggcac tttctctgca ttcttctttc
    10801 cagagcagat tgcctggtta agaatctctt gttgtcccct ttgtatattg ttattgtaaa
    10861 gtgccaaatg ccaggataca gccagaaaaa ttgcttatta ttattaaaaa aattttttta
    10921 agaaagacat ctggattgta gggtggactc gataacctgg tcattatttt tttgaagcca
    10981 aaatatccat ttatactatg tacctggtga ccagtgtctc tcattttaac tgagggtggt
    11041 gggtctgtgg atagaacact gactcttgct attttaatat caaagatatt ctagatccag
    11101 cacagtggcg gccgctctag agtggaactc ttaagaccag tatctttgtg tgggctttac
    11161 cagcattcac ttttagaaaa actacctaaa ttttataatc ctttaatttc ttcatctgga
    11221 gcacctgccc ctacttattt caagaagatt gcagtaaaac gattaaatga gggaacatat
    11281 gcagaggtgc ttttaaaaag catatgccac cttttttatt aattattata taaaatgaag
    11341 catttaatta tagtaataat ttgaagtagt ttgaagtacc acactgaggt gaggacttaa
    11401 aaatgataag acgagttccc tattttataa gaaaaataag ccaaaattaa atattctttt
    11461 ggatataaat ttcaacagtg agatagctgc ctagtggaaa tgaataatat cccagccgct
    11521 agtgtacagg gtgttttgtg gcacaggatt atgtaatatg gaactgctca agcaaataac
    11581 tagtcatcac aacagcagtt ctttgtaata actgaaaaag aatattgttt ctcggagaag
    11641 gatgtcaaaa gatcggccca gctcagggag cagtttgccc tactagctcc tcggacagct
    11701 gtaaagaaga gtctctggct ctttagaata ctgatcccat tgaagatacc acgctgcatg
    11761 tgtccttagt agtcatgtct ccttaggctc ctcttggaca ttctgagcat gtgagacctg
    11821 aggactgcaa acagctataa gaggctccaa attaatcata tctttccctt tgagaatctg
    11881 gccaagctcc agctaatcta cttggatggg ttgccagcta tctggagaaa aagatcttcc
    11941 tcagaagaat aggcttgttg ttttacagtg ttagtgatcc attccctttg acgatcccta
    12001 ggtggagatg gggcatgagg atcctccagg ggaaaagctc actaccactg ggcaacaacc
    12061 ctaggtcagg aggttctgtc aagatacttt cctggtccca gataggaaga taaagtctca
    12121 aaaacaacca ccacacgtca agctcttcat tgttcctatc tgccaaatca ttatacttcc
    12181 tacaagcagt gcagagagct gagtcttcag caggtccaag aaatttgaac acactgaagg
    12241 aagtcagcct tcccacctga agatcaacat gcctggcact ctagcacttg aggatagctg
    12301 aatgaatgtg tatttctttg tctctttctt tcttgtcttt gctctttgtt ctctatctaa
    12361 agtgtgtctt acccatttcc atgtttctct tgctaatttc tttcgtgtgt gcctttgcct
    12421 cattttctct ttttgttcac aagagtggtc tgtgtcttgt cttagacata tctctcattt
    12481 ttcattttgt tgctatttct ctttgctctc ctagatgtgg ctcttctttc acgctttatt
    12541 tcatgtctcc tttttgggtc acatgctgtg tgctttttgt ccttttcttg ttctgtctac
    12601 ctctcctttc tctgcctacc tctcttttct ctttgtgaac tgtgattatt tgttacccct
    12661 tccccttctc gttcgtttta aatttcacct tttttctgag tctggcctcc tttctgctgt
    12721 ttctactttt tatctcacat ttctcatttc tgcatttcct ttctgcctct cttgggctat
    12781 tctctctctc ctcccctgcg tgcctcagca tctcttgctg tttgtgattt tctatttcag
    12841 tattaatctc tgttggcttg tatttgttct ctgcttcttc cctttctact cacctttgag
    12901 tatttcagcc tcttcatgaa tctatctccc tctctttgat ttcatgtaat ctctccttaa
    12961 atatttcttt gcatatgtgg gcaagtgtac gtgtgtgtgt gtcatgtgtg gcagaggggc
    13021 ttcctaaccc ctgcctgata ggtgcagaac gtcggctatc agagcaagca ttgtggagcg
    13081 gttccttatg ccaggctgcc atgtgagatg atccaagacc aaaacaaggc cctagactgc
    13141 agtaaaaccc agaactcaag tagggcagaa ggtggaaggc tcatatggat agaaggccca
    13201 aagtataaga cagatggttt gagacttgag acccgaggac taagatggaa agcccatgtt
    13261 ccaagataga tagaagcctc aggcctgaaa ccaacaaaag cctcaagagc caagaaaaca
    13321 gagggtggcc tgaattggac cgaaggcctg agttggatgg aagtctcaag gcttgagtta
    13381 gaagtcttaa gacctgggac aggacacatg gaaggcctaa gaactgagac ttgtgacaca
    13441 aggccaacga cctaagatta gcccagggtt gtagctggaa gacctacaac ccaaggatgg
    13501 aaggcccctg tcacaaagcc tacctagatg gatagaggac ccaagcgaaa aaggtatctc
    13561 aagactaacg gccggaatct ggaggcccat gacccagaac ccaggaagga tagaagcttg
    13621 aagacctggg gaaatcccaa gatgagaacc ctaaacccta cctcttttct attgtttaca
    13681 cttcttactc ttagatattt ccagttctcc tgtttatctt taagcctgat tcttttgaga
    13741 tgtacttttt gatgttgccg gttaccttta gattgacagt attatgcctg ggccagtctt
    13801 gagccagctt taaatcacag cttttaccta tttgttaggc tatagtgttt tgtaaacttc
    13861 tgtttctatt cacatcttct ccacttgaga gagacaccaa aatccagtca gtatctaatc
    13921 tggcttttgt taacttccct caggagcaga cattcatata ggtgatactg tatttcagtc
    13981 ctttcttttg accccagaag ccctagactg agaagataaa atggtcaggt tgttggggaa
    14041 aaaaaaagtg ccaggctctc tagagaaaaa tgtgaagaga tgctccaggc caatgagaag
    14101 aattagacaa gaaatacaca gatgtgccag acttctgaga agcacctgcc agcaacagct
    14161 tccttctttg agcttagtcc atccctcatg aaaaatgact gaccactgct gggcagcagg
    14221 agggatgatg accaactaat tcccaaaccc cagtctcatt ggtaccatcg atcggccgga
    14281 tatcacgcgt catatggcta gcctgcaggg atccaatgta actgtattca gcgatgacga
    14341 aattcttagc tattgtaata ctctagagga tctttgtgaa ggaaccttac ttctgtggtg
    14401 tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt
    14461 taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct
    14521 atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct
    14581 cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc
    14641 caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt
    14701 tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg
    14761 aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta
    14821 ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt
    14881 ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg
    14941 ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca
    15001 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
    15061 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
    15121 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
    15181 ttgtccaaac tcatcaatgt atcttatcat gtctgcggct ctagagctgc attaatgaat
    15241 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
    15301 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
    15361 aatacggtta tccacagaat caggggataa cgcaggaaag aacattacgt aaactggcaa
    15421 aggggtggct gggccaaaag acagaggaat taagtaagaa gtccaggaaa aatgaacttc
    15481 acatcaaatt ttagagcacg gtagccatga atcttgtgaa tagctcccaa aaatgtcctg
    15541 tggaagacaa ctagaaagca ttctacaatc aggcacccac ctccacctgc agcctcctgt
    15601 gttgttctca tggggcacct ctgggctcca gctcctccaa ggcacctcca cactctctca
    15661 agtacactct tcactcttcc ccaaacatga ttcccctact gctctgccta actcccactt
    15721 ctctttcaag tagcagctta aacgtcacct catatttggc tggaaaatag aatatagaca
    15781 gaggggtaag ttaaggctag aaaggcaggc tgggtcaaca gaatggcaag ctaaaacatg
    15841 ggattttcta aaacagccta agagggtgcc agataaaagt gtgcaaggag tggcacaact
    15901 ccagtttcat ctttagctat agcaattaac accataagga gtctggattc aattttgcca
    15961 tttactagct agctaccaac ttctgtgtcg ctttgggcaa atcaattaaa tccatacctc
    16021 cctttccatc tgcagaatgg gtttataaca gtacttaaac ctcaaggtac taagaacagt
    16081 aaagagttaa tggtaatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
    16141 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
    16201 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
    16261 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
    16321 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
    16381 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
    16441 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
    16501 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
    16561 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
    16621 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
    16681 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
    16741 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
    16801 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
    16861 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
    16921 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
    16981 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
    17041 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
    17101 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
    17161 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
    17221 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
    17281 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
    17341 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
    17401 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
    17461 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
    17521 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    17581 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    17641 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    17701 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    17761 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    17821 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    17881 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
    17941 tataaaaata ggcgtatcac gaggcccttt cgtcttcaag aattcgaaaa ccagaaagta
    18001 ttctcagtaa tgatagtatg gataaagcag gtttctatga ccctttatta cagaatctgt
    18061 gagtttttca caattaaaaa gtaataaaaa gtagtgacaa cattcactga actcttattc
    18121 tatgccaact tgttccggta tgcccttaca cccacaaaag ccctatgcat aaggtggcat
    18181 tattccagca tgtattgcat tgtacacaca aagaggtcaa gcactccacc acggccctaa
    18241 gcatggtggc tgaggtggga aggccagagg taggtgggcc cgcgcccttt tccactctga
    18301 accatgcctc caagatagga gggtgggaaa gtgctcaaga cacattagaa attccccata
    18361 aaagacaaga ttgttgaaca cctgcaagtg aataaagata aactgatctc agaggggaaa
    18421 aagacgcagg gttaggaaac agcaccctgc tcgaggacgt tctttccaaa cagcctgctc
    18481 atcacccgtt cgaattc
//
