LOCUS       sequence-305311-       13425 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing Human ACE2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13425)
  TITLE     Best Lab Plasmids
REFERENCE   2  (bases 1 to 13425)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pWPI-IRES-Puro-Ak-ACE2
FEATURES             Location/Qualifiers
     source          1..13425
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    183..416
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             601..645
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1028..2206
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1259..2197
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2154..2174
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    2254..2371
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    4896..5463
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4914..5465
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5058..5075)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5285..5304
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5468..6067
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5468..5487)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5964..5984
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(6109..6125)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6110..6126)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    6139..6727
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6192..6212)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6610..6621)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(6871..6904)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             6924..7104
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7129..7147)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7130..7147)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7437..7456)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7670..7689)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7789..7811
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7849..7867)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7935..8039
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8040..8108,8109..8900)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8258..8277)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9071..9659
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9560..9579
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9813..9830
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9900..9920)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9905..10234
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10085..10220
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11368..11433
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11563..11583
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    12008..12142
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12045..12064)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12099..12118
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             12310..12943
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    12990..13115
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
       61 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
      121 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
      181 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
      241 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
      301 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
      361 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
      421 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
      481 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
      541 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
      601 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
      661 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
      721 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
      781 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
      841 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
      901 tatcgatcac gagactagcc tcgaccatcg atgtcgacga taagctttgc aaagatggat
      961 aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct tgaaaggagt
     1021 gggaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
     1081 gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg
     1141 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat
     1201 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt
     1261 aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc
     1321 ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc gggttggaag
     1381 tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg cttgagttga
     1441 ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct
     1501 cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt
     1561 tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta tttcggtttt
     1621 tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg
     1681 cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc ggcctgctct
     1741 ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc
     1801 ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag ggagctcaaa
     1861 atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa ggaaaagggc
     1921 ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc cgtccaggca
     1981 cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg aggggtttta
     2041 tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag cttggcactt
     2101 gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca ttctcaagcc
     2161 tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgaggaa tttcgacatt
     2221 taaatttaat taatctcgac ggtatcggtt aacttttaaa agaaaagggg ggattggggg
     2281 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     2341 acaaaaacaa attacaaaaa ttcaaaattt tatcgatcac gagactagcc tcgaggttta
     2401 aacccgggag cgccaccatg tcaagctctt cctggctcct tctcagcctt gttgctgtaa
     2461 ctgctgctca gtccaccatt gaggaacagg ccaagacatt tttggacaag tttaaccacg
     2521 aagccgaaga cctgttctat caaagttcac ttgcttcttg gaattataac accaatatta
     2581 ctgaagagaa tgtccaaaac atgaataatg ctggggacaa atggtctgcc tttttaaagg
     2641 aacagtccac acttgcccaa atgtatccac tacaagaaat tcagaatctc acagtcaagc
     2701 ttcagctgca ggctcttcag caaaatgggt cttcagtgct ctcagaagac aagagcaaac
     2761 ggttgaacac aattctaaat acaatgagca ccatctacag tactggaaaa gtttgtaacc
     2821 cagataatcc acaagaatgc ttattacttg aaccaggttt gaatgaaata atggcaaaca
     2881 gtttagacta caatgagagg ctctgggctt gggaaagctg gagatctgag gtcggcaagc
     2941 agctgaggcc attatatgaa gagtatgtgg tcttgaaaaa tgagatggca agagcaaatc
     3001 attatgagga ctatggggat tattggagag gagactatga agtaaatggg gtagatggct
     3061 atgactacag ccgcggccag ttgattgaag atgtggaaca tacctttgaa gagattaaac
     3121 cattatatga acatcttcat gcctatgtga gggcaaagtt gatgaatgcc tatccttcct
     3181 atatcagtcc aattggatgc ctccctgctc atttgcttgg tgatatgtgg ggtagatttt
     3241 ggacaaatct gtactctttg acagttccct ttggacagaa accaaacata gatgttactg
     3301 atgcaatggt ggaccaggcc tgggatgcac agagaatatt caaggaggcc gagaagttct
     3361 ttgtatctgt tggtcttcct aatatgactc aaggattctg ggaaaattcc atgctaacgg
     3421 acccaggaaa tgttcagaaa gcagtctgcc atcccacagc ttgggacctg gggaagggcg
     3481 acttcaggat ccttatgtgc acaaaggtga caatggacga cttcctgaca gctcatcatg
     3541 agatggggca tatccagtat gatatggcat atgctgcaca accttttctg ctaagaaatg
     3601 gagctaatga aggattccat gaagctgttg gggaaatcat gtcactttct gcagccacac
     3661 ctaagcattt aaaatccatt ggtcttctgt cacccgattt tcaagaagac aatgaaacag
     3721 aaataaactt cctgctcaaa caagcactca cgattgttgg gactctgcca tttacttaca
     3781 tgttagagaa gtggaggtgg atggtcttta aaggggaaat tcccaaagac cagtggatga
     3841 aaaagtggtg ggagatgaag cgagagatag ttggggtggt ggaacctgtg ccccatgatg
     3901 aaacatactg tgaccccgca tctctgttcc atgtttctaa tgattactca ttcattcgat
     3961 attacacaag gaccctttac caattccagt ttcaagaagc actttgtcaa gcagctaaac
     4021 atgaaggccc tctgcacaaa tgtgacatct caaactctac agaagctgga cagaaactgt
     4081 tcaatatgct gaggcttgga aaatcagaac cctggaccct agcattggaa aatgttgtag
     4141 gagcaaagaa catgaatgta aggccactgc tcaactactt tgagccctta tttacctggc
     4201 tgaaagacca gaacaagaat tcttttgtgg gatggagtac cgactggagt ccatatgcag
     4261 accaaagcat caaagtgagg ataagcctaa aatcagctct tggagataaa gcatatgaat
     4321 ggaacgacaa tgaaatgtac ctgttccgat catctgttgc atatgctatg aggcagtact
     4381 ttttaaaagt aaaaaatcag atgattcttt ttggggagga ggatgtgcga gtggctaatt
     4441 tgaaaccaag aatctccttt aatttctttg tcactgcacc taaaaatgtg tctgatatca
     4501 ttcctagaac tgaagttgaa aaggccatca ggatgtcccg gagccgtatc aatgatgctt
     4561 tccgtctgaa tgacaacagc ctagagtttc tggggataca gccaacactt ggacctccta
     4621 accagccccc tgtttccata tggctgattg tttttggagt tgtgatggga gtgatagtgg
     4681 ttggcattgt catcctgatc ttcactggga tcagagatcg gaagaagaaa aataaagcaa
     4741 gaagtggaga aaatccttat gcctccatcg atattagcaa aggagaaaat aatccaggat
     4801 tccaaaacac tgatgatgtt cagacctcct tttagggatc catatgcgga ccgtacgtac
     4861 tagtggccac ggctgatcat acgggctgca ggaattccgc cccccccccc ctaacgttac
     4921 tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat
     4981 attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat
     5041 tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga
     5101 agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca
     5161 gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac
     5221 acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt
     5281 caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca
     5341 ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt
     5401 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga
     5461 taataccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
     5521 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     5581 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     5641 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     5701 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     5761 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     5821 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     5881 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     5941 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     6001 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     6061 tgcctgacgg gcgcgtctgg aacttcgaat tcgatatcaa gcttatcgcg ataccgtcga
     6121 cctcgaggga attccgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     6181 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     6241 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     6301 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     6361 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     6421 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     6481 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     6541 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     6601 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     6661 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     6721 tccccgcatc gggaattcga gctcggtacc tttaagacca atgacttaca aggcagctgt
     6781 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     6841 aagacaagat gggatcaatt caccatggga ataacttcgt atagcataca ttatacgaag
     6901 ttatgctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     6961 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     7021 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     7081 agtcagtgtg gaaaatctct agcagcatct agaattaatt ccgtgtattc tatagtgtca
     7141 cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc gttctaacga
     7201 caatatgtac aagcctaatt gtgtagcatc tggcttactg aagcagaccc tatcatctct
     7261 ctcgtaaact gccgtcagag tcggtttggt tggacgaacc ttctgagttt ctggtaacgc
     7321 cgtcccgcac ccggaaatgg tcagcgaacc aatcagcagg gtcatcgcta gccagatcct
     7381 ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta
     7441 tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg
     7501 tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt
     7561 gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt
     7621 cctaatgcag gagtcgcata agggagagcg tcgaatggtg cactctcagt acaatctgct
     7681 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     7741 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     7801 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     7861 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     7921 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     7981 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     8041 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     8101 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     8161 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     8221 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     8281 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     8341 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     8401 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     8461 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     8521 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     8581 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     8641 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     8701 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     8761 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     8821 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     8881 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     8941 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     9001 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     9061 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     9121 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     9181 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     9241 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     9301 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     9361 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     9421 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     9481 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     9541 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     9601 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     9661 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     9721 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     9781 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     9841 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgtg
     9901 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     9961 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
    10021 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
    10081 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
    10141 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
    10201 aggaggcttt tttggaggcc taggcttttg caaaaagctt ggacacaaga caggcttgcg
    10261 agatatgttt gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg
    10321 cggactcatg tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta
    10381 ttttcccctc gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg
    10441 aaaaatacat cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac
    10501 tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg
    10561 tgcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta
    10621 cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg
    10681 cttcatcgtt attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat
    10741 gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga
    10801 tgactatgtt gttgatatcc cgcaagatac ctggattgaa cagccgtggg atatgggcgt
    10861 cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt
    10921 tgttcttttt aacttcaggc gggttacaat agtttccagt aagtattctg gaggctgcat
    10981 ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa
    11041 cctcgacgct agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg
    11101 atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg
    11161 gcattgaccc acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac
    11221 gtaccgacga tgatttatac gatacggtga ttggctaccg tggcggcaac tggatttatg
    11281 agtgggcccc ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa
    11341 ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt
    11401 aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg
    11461 gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct
    11521 agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag
    11581 gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt
    11641 agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta
    11701 tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat
    11761 cataacatac tgttttttct tactccacac aggcatagag tgtctgctat taataactat
    11821 gctcaaaaat tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg
    11881 atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag aggttttact
    11941 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
    12001 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
    12061 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
    12121 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
    12181 tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg
    12241 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa
    12301 cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga
    12361 tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca
    12421 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag
    12481 aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg
    12541 atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg
    12601 tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg
    12661 gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag
    12721 ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac
    12781 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
    12841 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
    12901 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
    12961 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
    13021 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
    13081 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
    13141 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
    13201 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
    13261 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
    13321 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
    13381 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaa
//
