LOCUS       sequence-30538-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Lei K, Davis RJ
  TITLE     JNK phosphorylation of Bim-related members of the Bcl2 family 
            induces Bax-dependent apoptosis.
  JOURNAL   Proc Natl Acad Sci U S A. 2003 Mar 4. 100(5):2432-7.
  PUBMED    12591950
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2003 Mar 4. 100(5):2432-7."
COMMENT     Sequence Label: pCDNA3 Flag BimL (T56D)
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..74
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        55..73
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             102..125
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(606..624)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(607..624)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(644..661)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    650..763
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ttagcagagc tctctggcta ctagagaacc cactgcttac tggcttatcg aaattaatac
       61 gactcactat agggagaccc aagcttacgg cagccaacat ggactataag gacgatgatg
      121 acaaaggatc cgcaaagcaa ccttctgatg taagttctga gtgtgaccga gaaggtagac
      181 aattgcagcc tgcggagagg cctccccagc tcagacctgg ggcccctacc tccctacaga
      241 cagagccaca agacaggagc ccagcaccca tgagttgtga caaatcaaca caagacccaa
      301 gtcctccttg ccaggccttc aaccactatc tcagtgcaat ggcttccatg aggcaggctg
      361 aacctgcaga tatgcgccca gagatatgga tcgcccaaga gttgcggcgt atcggagacg
      421 agtttaacgc ttactatgca aggagggtat ttttgaataa ttaccaagca gccgaagacc
      481 acccacgaat ggttatctta cgactgttac gttacattgt ccgcctggtg tggagaatgc
      541 attgagaatt ctgcagatat ccatcacact ggcggccgct cgagcatgca tctagagggc
      601 cctattctat agtgtcacct aaatgctaga gctcgctgat cagcctcgac tgtgccttct
      661 agttgccagc catctngttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
      721 cactcnccac tgtcctttcc taataaaatg aggaaattgc atctcattgt ctgagtaggt
      781 gtcattctat tctgggggtg gggtggggca ggacagcang gggaggatgg gaagncatag
      841 caggctgctg ggng
//
