LOCUS       sequence-305389-         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens 
            at a single cell resolution (using cytometry)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Wroblewska A, Dhainaut M, Ben-Zvi B, Rose SA, Park ES, Amir ED, 
            Bektesevic A, Baccarini A, Merad M, Rahman AH, Brown BD
  TITLE     Protein Barcodes Enable High-Dimensional Single-Cell CRISPR Screens.
  JOURNAL   Cell. 2018 Nov 1;175(4):1141-1155.e16. doi: 
            10.1016/j.cell.2018.09.022. Epub 2018 Oct 18.
  PUBMED    30343902
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.09.022"; journalName: "Cell"; date: "2018-11-1-
            1"; volume: "175"; issue: "4"; pages: "1141-1155.e16"
COMMENT     Sequence Label: pLKO_FLAG.VSVg.C_NGFR
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             97..120
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             139..171
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             190..225
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
ORIGIN
        1 atgggggcag gtgccaccgg ccgcgccatg gacgggccgc gcctgctgct gttgctgctt
       61 ctgggggtgt cccttggagg tgccaaggag gcatgcgact acaaagacga tgacgacaag
      121 ggaggaggcg ggggagggta tacagatatc gaaatgaata gactcggaaa gggaggaggc
      181 gggggagggg aggaccaggt ggaccccagg ctgatcgacg gcaagcccac aggcctgtac
      241 acacacagcg gtgagtgctg caaagcctgc aacctgggcg agggtgtggc ccagccttgt
      301 ggagccaacc agaccgtgtg tgagccctgc ctggacagcg tgacgttctc cgacgtggtg
      361 agcgcgaccg agccgtgcaa gccgtgcacc gagtgcgtgg ggctccagag catgtcggcg
      421 ccgtgcgtgg aggccgacga cgccgtgtgc cgctgcgcct acggctacta ccaggatgag
      481 acgactgggc gctgcgaggc gtgccgcgtg tgcgaggcgg gctcgggcct cgtgttctcc
      541 tgccaggaca agcagaacac cgtgtgcgag gagtgccccg acggcacgta ttccgacgag
      601 gccaaccacg tggacccgtg cctgccctgc accgtgtgcg aggacaccga gcgccagctc
      661 cgcgagtgca cacgctgggc cgacgccgag tgcgaggaga tccctggccg ttggattaca
      721 cggtccacac ccccagaggg ctcggacagc acagccccca gcacccagga gcctgaggca
      781 cctccagaac aagacctcat agccagcacg gtggcaggtg tggtgaccac agtgatgggc
      841 agctcccagc ccgtggtgac ccgaggcacc accgacaacc tcatccctgt ctattgctcc
      901 atcctggctg ctgtggttgt gggccttgtg gcctacatag ccttcaagag gtggaacagg
      961 ggtatcctct ag
//
