LOCUS       sequence-30543-e         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Lei K, Davis RJ
  TITLE     JNK phosphorylation of Bim-related members of the Bcl2 family 
            induces Bax-dependent apoptosis.
  JOURNAL   Proc Natl Acad Sci U S A. 2003 Mar 4. 100(5):2432-7.
  PUBMED    12591950
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2003 Mar 4. 100(5):2432-7."
COMMENT     Sequence Label: pCDNA3 T7 BimL (AA)
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             82..114
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     primer_bind     complement(544..564)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        549..878
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      729..864
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     791..810
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 cgagctctct ggctactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct tatggctagc atgactggtg gacagcaaat gggtggatcc
      121 gcaaagcaac cttctgatgt aagttctgag tgtgaccgag aaggtagaca attgcagcct
      181 gcggagaggc ctccccagct cagacctggg gcccctacct ccctacagac agagccacaa
      241 gacaggagcc cagcacccat gagttgtgac aaatcaacac aagccccagc tcctccttgc
      301 caggccttca accactatct cagtgcaatg gcttccatga ggcaggctga acctgcagat
      361 atgcgcccag agatatggat cgcccaagag ttgcggcgta tcggagacga gtttaacgct
      421 tactatgcaa ggagggtatt tttgaataat taccaagcag ccgaagacca cccacgaatg
      481 gttatcttac gactgttacg ttacattgtc cgcctggtgt ggagaatgca ttgagaattc
      541 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
      601 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
      661 caggcagaag tatgcaaagc atgcatctca attagtcaca aaccatagtc ccgcccctaa
      721 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
      781 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
      841 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
      901 ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaaa
//
