LOCUS       sequence-305430-        8861 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens 
            at a single cell resolution (using cytometry)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8861)
  AUTHORS   Wroblewska A, Dhainaut M, Ben-Zvi B, Rose SA, Park ES, Amir ED, 
            Bektesevic A, Baccarini A, Merad M, Rahman AH, Brown BD
  TITLE     Protein Barcodes Enable High-Dimensional Single-Cell CRISPR Screens.
  JOURNAL   Cell. 2018 Nov 1;175(4):1141-1155.e16. doi: 
            10.1016/j.cell.2018.09.022. Epub 2018 Oct 18.
  PUBMED    30343902
REFERENCE   2  (bases 1 to 8861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.09.022"; journalName: "Cell"; date: "2018-11-1-
            1"; volume: "175"; issue: "4"; pages: "1141-1155.e16"
COMMENT     Sequence Label: pLKO_HSV.S.VSVg_NGFR
FEATURES             Location/Qualifiers
     source          1..8861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        50..298
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     50..70
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     221..240
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        317..392
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    440..557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        697..1874
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          928..1865
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1822..1842
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1923..1932
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2025..2057
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             2076..2120
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             2139..2171
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     misc_feature    2934..3522
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2987..3007)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3405..3416)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3597..3830
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3902..4023
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3939..3958)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3993..4012
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4063..4198
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4218..4237)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4219..4237)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4247..4264)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4247..4263)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4256..4278)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4405..4860
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4492..4511)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4702..4723
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4886..4990
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4991..5059,5060..5851)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5209..5228)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6022..6610
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6511..6530
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6764..6781
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6898..6919
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6934..6939,6940..6957,6958..6964)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6972..6988
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6977..6999
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6996..7012
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6996..7012
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7031..7051
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7033..7051
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7079..7305
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7306..7486
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7533..7658
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8151..8384
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8569..8613
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tcgacggtat cgatcacgag actagtatcg agcggccgcc cccttcaccg agggcctatt
       61 tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga taattggaat
      121 taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt
      181 tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt
      241 aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgg
      301 gtcttcgaga agacctgttt tagagctaga aatagcaagt taaaataagg ctagtccgtt
      361 atcaacttga aaaagtggca ccgagtcggt gcttttttta attctcgacc tcgagacaaa
      421 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
      481 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
      541 caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt tggccgcggc
      601 tcgaggcgat aagctttgca aagatggata aagttttaaa cagagaggaa tctttgcagc
      661 taatggacct tctaggtctt gaaaggagtg ggaattggct ccggtgcccg tcagtgggca
      721 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt
      781 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
      841 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
      901 cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc
      961 ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccactggctg cagtacgtga
     1021 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
     1081 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
     1141 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
     1201 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
     1261 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
     1321 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
     1381 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
     1441 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
     1501 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
     1561 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     1621 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     1681 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     1741 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     1801 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     1861 ttcaggtgtc gtgaggaatt ctgcagtcga tcgacggtac cgcgggcccg ggatccacgg
     1921 tcgccaccat gggggcaggt gccaccggcc gcgccatgga cgggccgcgc ctgctgctgt
     1981 tgctgcttct gggggtgtcc cttggaggtg ccaaggaggc atgccagccc gagctggccc
     2041 ccgaggaccc cgaggacgga ggaggcgggg gagggaagga gaccgccgcc gccaagttcg
     2101 agaggcagca catggacagc ggaggaggcg ggggagggta tacagatatc gaaatgaata
     2161 gactcggaaa gcccacaggc ctgtacacac acagcggtga gtgctgcaaa gcctgcaacc
     2221 tgggcgaggg tgtggcccag ccttgtggag ccaaccagac cgtgtgtgag ccctgcctgg
     2281 acagcgtgac gttctccgac gtggtgagcg cgaccgagcc gtgcaagccg tgcaccgagt
     2341 gcgtggggct ccagagcatg tcggcgccgt gcgtggaggc cgacgacgcc gtgtgccgct
     2401 gcgcctacgg ctactaccag gatgagacga ctgggcgctg cgaggcgtgc cgcgtgtgcg
     2461 aggcgggctc gggcctcgtg ttctcctgcc aggacaagca gaacaccgtg tgcgaggagt
     2521 gccccgacgg cacgtattcc gacgaggcca accacgtgga cccgtgcctg ccctgcaccg
     2581 tgtgcgagga caccgagcgc cagctccgcg agtgcacacg ctgggccgac gccgagtgcg
     2641 aggagatccc tggccgttgg attacacggt ccacaccccc agagggctcg gacagcacag
     2701 cccccagcac ccaggagcct gaggcacctc cagaacaaga cctcatagcc agcacggtgg
     2761 caggtgtggt gaccacagtg atgggcagct cccagcccgt ggtgacccga ggcaccaccg
     2821 acaacctcat ccctgtctat tgctccatcc tggctgctgt ggttgtgggc cttgtggcct
     2881 acatagcctt caagaggtgg aacaggggta tcctctagag cggccgcgtc gacaatcaac
     2941 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     3001 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     3061 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     3121 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     3181 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     3241 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     3301 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg
     3361 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     3421 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcat cttcgccttc
     3481 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgggtac ctttaagacc
     3541 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     3601 agggctaatt cactcccaac gaagataaga tctgcttttt gcttgtactg ggtctctctg
     3661 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     3721 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     3781 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     3841 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     3901 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     3961 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     4021 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     4081 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     4141 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     4201 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     4261 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     4321 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     4381 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     4441 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     4501 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     4561 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     4621 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     4681 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     4741 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     4801 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     4861 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     4921 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     4981 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     5041 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     5101 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     5161 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     5221 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     5281 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     5341 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     5401 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     5461 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     5521 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     5581 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     5641 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     5701 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     5761 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     5821 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     5881 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     5941 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     6001 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     6061 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     6121 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     6181 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     6241 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     6301 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     6361 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     6421 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     6481 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     6541 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     6601 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     6661 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     6721 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     6781 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     6841 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     6901 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     6961 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     7021 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     7081 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     7141 ttacaaggag agaaaaagca ccgtgcatgg cgattggtgg aagtaaggtg gtacgatcgt
     7201 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     7261 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     7321 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     7381 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     7441 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     7501 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     7561 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     7621 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     7681 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     7741 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     7801 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     7861 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     7921 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     7981 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     8041 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     8101 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     8161 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     8221 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     8281 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     8341 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     8401 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     8461 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     8521 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     8581 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     8641 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     8701 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     8761 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     8821 gagagagaca gagacagatc cattcgatta gtgaacggat c
//
