LOCUS       sequence-305519-         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens 
            at a single cell resolution (using cytometry)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Wroblewska A, Dhainaut M, Ben-Zvi B, Rose SA, Park ES, Amir ED, 
            Bektesevic A, Baccarini A, Merad M, Rahman AH, Brown BD
  TITLE     Protein Barcodes Enable High-Dimensional Single-Cell CRISPR Screens.
  JOURNAL   Cell. 2018 Nov 1;175(4):1141-1155.e16. doi: 
            10.1016/j.cell.2018.09.022. Epub 2018 Oct 18.
  PUBMED    30343902
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.09.022"; journalName: "Cell"; date: "2018-11-1-
            1"; volume: "175"; issue: "4"; pages: "1141-1155.e16"
COMMENT     Sequence Label: pLKO_NWS.Ollas.HA_NGFR
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             100..123
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             142..183
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             205..231
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 atgggggcag gtgccaccgg ccgcgccatg gacgggccgc gcctgctgct gttgctgctt
       61 ctgggggtgt cccttggagg tgccaaggag gcatgcaact ggtcccaccc ccaattcgag
      121 aaaggaggag gcgggggagg gagcggcttc gccaacgagc tgggccccag gctgatgggc
      181 aagtgcggag gaggcggggg agggtaccca tacgatgtgc cagattacgc tcccacaggc
      241 ctgtacacac acagcggtga gtgctgcaaa gcctgcaacc tgggcgaggg tgtggcccag
      301 ccttgtggag ccaaccagac cgtgtgtgag ccctgcctgg acagcgtgac gttctccgac
      361 gtggtgagcg cgaccgagcc gtgcaagccg tgcaccgagt gcgtggggct ccagagcatg
      421 tcggcgccgt gcgtggaggc cgacgacgcc gtgtgccgct gcgcctacgg ctactaccag
      481 gatgagacga ctgggcgctg cgaggcgtgc cgcgtgtgcg aggcgggctc gggcctcgtg
      541 ttctcctgcc aggacaagca gaacaccgtg tgcgaggagt gccccgacgg cacgtattcc
      601 gacgaggcca accacgtgga cccgtgcctg ccctgcaccg tgtgcgagga caccgagcgc
      661 cagctccgcg agtgcacacg ctgggccgac gccgagtgcg aggagatccc tggccgttgg
      721 attacacggt ccacaccccc agagggctcg gacagcacag cccccagcac ccaggagcct
      781 gaggcacctc cagaacaaga cctcatagcc agcacggtgg caggtgtggt gaccacagtg
      841 atgggcagct cccagcccgt ggtgacccga ggcaccaccg acaacctcat ccctgtctat
      901 tgctccatcc tggctgctgt ggttgtgggc cttgtggcct acatagcctt caagaggtgg
      961 aacaggggta tcctctag
//
