LOCUS       sequence-306042-       14149 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, 
            activating DsRed-SKL with promoter CP_pcbAB (no QUAS control).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14149)
  AUTHORS   Mozsik L, Buttel Z, Bovenberg RAL, Driessen AJM, Nygard Y
  TITLE     Synthetic control devices for gene regulation in Penicillium 
            chrysogenum.
  JOURNAL   Microb Cell Fact. 2019 Nov 18;18(1):203. doi: 
            10.1186/s12934-019-1253-3.
  PUBMED    31739777
REFERENCE   2  (bases 1 to 14149)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2019-11-18"; volume: "18(1):203. doi"; pages: " 
            10.1186/s12934-019-1253-3"
COMMENT     Sequence Label: pVE2_9_p40S_QfDBD_VP16AD_cp_pcbAB_DsRed_0xQUAS
FEATURES             Location/Qualifiers
     source          1..14149
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2781..2891
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     CDS             join(2904..2906,2907..2909,2910..3620)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2904..2906,2907..2909,2910..3620)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2949..2970)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3210..3229)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3557..3578
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3621..3641
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          4813..4928
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     CDS             7419..8093
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(7549..7567)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(7599..7619)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     8011..8034
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    9927..9951
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      10039..10749
                     /label=oriV
                     /note="incP origin of replication"
     promoter        10770..10874
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10875..11669
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      11840..12428
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12329..12348
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             12764..13912
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    14058..14082
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gcctgcagga tgggcctcca caaccctgcc ctaacattgt
       61 tcgtagaaga tctgccagca ggcttatgtc caggcccctc tcaacccgcc ctttcctttc
      121 agctcgggcg ccgtacttgc cccacccagg cgtgttccgc tgaactgccc atacccaggc
      181 cagcggactg ataaccattt ctctttatgc tttgctattg ggcttgtgac tcgttcttgg
      241 aggcaaggtt atcagattga atagaagatt cggtgatgca gcaaatagcg actgttcgtt
      301 gcggggtccg aacccgctcg gcagcaccgg gctctcccta ctatccctcg atagcagtgg
      361 cgtgcgcggg ctgtcgaccc tgtccatcct gaaagagttg atattgaagg tagtaggtac
      421 ggtgcagggt aacgcgtgat atcctgtcct cagtcttaag acttctcacc tatatcaccc
      481 gcacttaccc tagagtgccg cttaggtgct aagggcacat tgagtattgg ccgtgtagaa
      541 tatatagctt aagtacggcc aagcagacgg gaagccctgt tctccacacc ctatggtcgt
      601 atatatcagg cttctaccgg gaaacgatta agagtgtata atggactgaa aatcaatatg
      661 aacgggacaa tgctcaagtt aaattagtta ggcatcctaa tctctactaa atgttctatc
      721 tagagatcgg ggtactatag gcccgtacgt taatcactct acgcttctct cccttaggta
      781 tagtgtaggt aggggctaga catttatatg agtcagatgg tacaaacggt agcgctgcaa
      841 gaattcaagc ttggaggagt tatagacggt ccggcatagg taaggagaga ttagatattt
      901 cgacttcccg attccttagt ctccttagtc tggaaactga gaaaggcctg aactcgttca
      961 atctattagc tctaatcacg tagtctcaac tagaatcctg aacaggcgcc ggaaccagtc
     1021 caaatgcgtg gagtacagca agtccagtca aatcacgatt agaatggcca tatataagca
     1081 aaaaaccctt ggacaatacg tccgatcgac accaaagcaa tctggtacat cacccttctt
     1141 atatacaggg tagccggaat tgtattgact ctatacgaag ccgcaggttt accagcacag
     1201 aggtgcgata gctacacgaa tgtagagtga aggcattaca accgattgct aagtgtcata
     1261 gaggctaagc ttcacggctc aaggagtctg tggaggatac ctgatataag aattgagagt
     1321 tatactccgg aggttatgtt agtacctcaa aaatgtgaag tacaacgaaa ctcgaacttc
     1381 gaatgacaat agcttggctg gctgttagtc aactgaggta caacgtcatc ttgcaattag
     1441 ttatgttctt tgctagatag tgagctgctg taaacttgta gttcttagaa acatgtctga
     1501 agtctagagt cgagacttgt gattggtgta aattagtggg ggtccggagc ggaataacaa
     1561 tcgaggtcag cctcctgggt ctgtcagtaa gctaaccacg aaaggacaag cgtagacgag
     1621 gtcacggggt actggcctag ccggcttcgg ctagcgccca cgaaaatagg gcaacgtcga
     1681 agcgagagac acacacggat tccacgatcg ccgacgacaa gcactcaact ccacgacaac
     1741 ccacgacaac ccttgccgct gcgttcttgg tgaagggctg caggattaga ggtgagtatt
     1801 ttattttctg gctattgtga tctttttccc ccccggtttc cgctggttct atcttgtcga
     1861 gcgagtcggt gcgccttcga tcggtctgtc aaatttccat ccgtccactc gttcacccgg
     1921 tatcgataca agagggaatg gagcattacg acaggggagc agaggcagaa ctctcgatgt
     1981 tgctactttt tttgcttgga acaacttcct tgaaagattc ttttctgaat gcggctggga
     2041 aactgcgaat tggatatcca gcgaaaggtt accgacagac tcacggccat cgataccact
     2101 cagcgggata gaattgaagg cattcggaca ttagatgatc agcttcgctt gtttcgactc
     2161 gtgctaatca attatccctg gacagtcccc cacatagaca gcaaacaatg ccacccaagc
     2221 gcaaaacgct taacgctgcg gctgaggcta acgctcatgc cgacggacac gccgacggaa
     2281 acgccgacgg acacgtggcc aatacggccg cgtcctcgaa taatgcgagg ttcgctgatc
     2341 tcactaacat cgatactccg ggtctgggac ccacaactac gaccctgctc gtggaaccag
     2401 cacgctcaaa gcgtcaacga gtgtcccgcg catgcgacca gtgccgtgca gcccgagaga
     2461 aatgcgacgg aatacagcct gcgtgtttcc cgtgcgtttc ccagggaagg tcctgcactt
     2521 atcaggcttc gccgaaaaag aggggagttc aaaccggtta tattcgtacg ctggagctcg
     2581 ccctcgcctg gatgtttgaa aatgtcgcgc gttccgaaga tgccttgcat aacctcctcg
     2641 tccgtgacgc cggacaagga tcagctctgc tcgttggtaa agattcgccg gctgccgagc
     2701 gactccatgc ccgttgggct actagccgtg tcaataagag cattacccgc ctcctcaggt
     2761 ttgaaagcgg cgggccggcc gacgcccttg acgattttga cttagacatg ctcccagccg
     2821 atgcccttga cgactttgac cttgatatgc tgcctgctga cgctcttgac gattttgacc
     2881 ttgacatgct ccccgggttc gttatggtga gcaagggcga ggagctgttc accggggtgg
     2941 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     3001 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     3061 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
     3121 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     3181 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     3241 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     3301 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     3361 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     3421 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     3481 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
     3541 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     3601 gcatggacga gctgtacaag ccaaagaaga agcggaaggt ctaagcttac ttctttatcg
     3661 gttctctctt acgacttttt gaatggaacg tttccttctt ctcaggcggg cctatctttg
     3721 ggccgaagct cttttccttg tactgtagga cctggttgat aatgattccc aaaaagacat
     3781 ccagcatgtc agttacttgc attcgtcagt ctatacaaaa gcaatggttt agagaaattt
     3841 tgaactttat acatggtttt atttgttgct tcacggccgt accttctgga aatccacggt
     3901 aggagtgtca atttgcgttt ttgataatcc ttccaaggtt cttctcgaag tagttgttct
     3961 ataattgctt cacagctacc atggaacatc ccaagcaccg ctactagaat tcgagctcgg
     4021 agtggatccc ccgggctgca ggaattcgag ctctgtacag tgaccggtga ctctttctgg
     4081 catgcggaga gacggacgga cgcagagaga agggctgagt aataagcgcc actgcgccag
     4141 acagctctgg cggctctgag gtgcagtgga tgattattaa tccgggaccg gccgcccctc
     4201 cgccccgaag tggaaaggct ggtgtgcccc tcgttgacca agaatctatt gcatcatcgg
     4261 agaatatgga gcttcatcga atcaccggca gtaagcgaag gagaatgtga agccaggggt
     4321 gtatagccgt cggcgaaata gcatgccatt aacctaggta cagaagtcca attgcttccg
     4381 atctggtaaa agattcacga gatagtacct tctccgaagt aggtagagcg agtacccggc
     4441 gcgtaagctc cctaattggc ccatccggca tctgtagggc gtccaaatat cgtgcctctc
     4501 ctgctttgcc cggtgtatga aaccggaaag gccgctcagg agctggccag cggcgcagac
     4561 cgggaacaca agctggcagt cgacccatcc ggtgctctgc actcgacctg ctgaggtccc
     4621 tcagtccctg gtaggcagct ttgccccgtc tgtccgcccg gtgtgtcggc ggggttgaca
     4681 aggtcgttgc gtcagtccaa catttgttgc catattttcc tgctctcccc accagctgct
     4741 cttttctttt ctctttcttt tcccatcttc agtatattca tcttcccatc caagaacctt
     4801 tatttcccct aagtaagtac tttgctacat ccatactcca tccttcccat cccttattcc
     4861 tttgaacctt tcagttcgag ctttcccact tcatcgcagc ttgactaaca gctaccccgc
     4921 ttgagcagac atcaccatgc ctcaatcctg ggaagaactg gccgctgata agcgcgcccg
     4981 cctcgcaaaa accatccctg atgaatggaa agtccagacg ctgcctgcgg atgacagcgt
     5041 tattgatttc ccaaagaaat cggggatcct ttcagaggcc gaactgaaga tcacagaggc
     5101 ctccgctgca gatcttgtgt ccaagctggc ggccggagag ttgacctcgg tggaagttac
     5161 gctagcattc tgtaaacggg cagcaatcgc ccagcagtta gtagggtccc ctctacctct
     5221 cagggagatg taacaacgcc accttatggg actatcaagc tgacgctggc ttctgtgcag
     5281 acaaactgcg cccacgagtt cttccctgac gccgctctcg cgcaggcaag ggaactcgat
     5341 gaatactacg caaagcacaa gacacccgtt ggtccactcc atggcctccc catctctctc
     5401 aaagaccagc ttcgagtcaa ggtacaccgt tgcccctaag tcgttagatg tccctttttg
     5461 tcagctaaca tatgccacca gggctacgaa acatcaatgg gctacatctc atggctaaac
     5521 aagtacgacg aaggggactc ggttctgaca accatgctcc gcaaagccgg tgccgttttc
     5581 tacgtcaaga cctctgtccc gcagaccctg atggtctgcg agacagtcaa caacatcatc
     5641 gggcgcaccg tcaacccacg caacaagaac tggtcgtgcg gcggcagttc tggtggtgag
     5701 ggtgcgatcg ttgggattcg tggtggcgtc atcggtgtag gaacggatat cggtggctcg
     5761 attcgagtgc cggccgcgtt caacttcctg tacggtctaa ggccgagtca tgggcggctg
     5821 ccgtatgcaa agatggcgaa cagcatggag ggtcaggaga cggtgcacag cgttgtcggg
     5881 ccgattacgc actctgttga gggtgagtcc ttcgcctctt ccttcttttc ctgctctata
     5941 ccaggcctcc actgtcctcc tttcttgctt tttatactat atacgagacg ggcagtcact
     6001 gatgaagtaa tgttagacct ccgcctcttc accaaatccg tcctcggtca ggagccatgg
     6061 aaatacgact ccaaggtcat ccccatgccc tggcgccagt ccgagtcgga cattattgcc
     6121 tccaagatca agaacggcgg gctcaatatc ggctactaca acttcgacgg caatgtcctt
     6181 ccacaccctc ctatcctgcg cggcgtggaa accaccgtcg ccgcactcgc caaagccggt
     6241 cataccgtga ccccgtggac gccatacaag cacgatttcg gccacgatct catctcccat
     6301 atctacgcgg ctgacggcag cgccgacgta atgcgcgata tcagtgcatc cggcgagccg
     6361 gcgattccaa atatcaaaga cctactgaac ccgaacatca aagctgttaa catgaacgag
     6421 ctctgggaca cgcatctcca gaagtggaat taccagatgg agtaccttga gaaatggcgg
     6481 gaggctgaag aaaaggccgg gaaggaactg gacgccatca tcgcgccgat tacgcctacc
     6541 gctgcggtac ggcatgacca gttccggtac tatgggtatg cctctgtgat caacctgctg
     6601 gatttcacga gcgtggttgt tccggttacc tttgcggata agaacatcga taagaagaat
     6661 gagagtttca aggcggttag tgagcttgat gccctcgtgc aggaagagta tgatccggag
     6721 gcgtaccatg gggcaccggt tgcagtgcag gttatcggac ggagactcag tgaagagagg
     6781 acgttggcga ttgcagagga agtggggaag ttgctgggaa atgtggtgac tccataggct
     6841 tctaataagt gtcagatagc aatttgcaca agaaatcaat accagcaact gtaaataagc
     6901 gctgaagtga ccatgccatg ctacgaaaga gcagaaaaaa acctgccgta gaaccgaaga
     6961 gatatgacac gcttccatct ctcaaaggaa gaatcccttc agggttgcgt ttccagtcta
     7021 gacacgtata acggcacaag tgtctctcac caaatgggtt atatctcaaa tgtgatctaa
     7081 ggatggaaag cccagaatat tggctgggtt gatggctgct tcgagtgcag tctcatgctg
     7141 ccacaggtga ctctggatgg ccccatacca ctcaacccat ggtacgagcg gtacgcttta
     7201 cgaattccca tggggagatc ttgtctgcgg gcagtgcttc agtcgcccag attctcgatg
     7261 gagattggcc aggtcagcca tatataccct gcaatggcag accaatgcag caggcccagt
     7321 ataaggaatt cccctcgagc ttgtctgtga ttgcgttttt tctaacactt gttgttgcat
     7381 ccgatccgtc cctaccaatt attggtcatt gacagacaat ggcctcctcc gaggacgtca
     7441 tcaaggagtt catgcgcttc aaggtgcgca tggagggctc cgtgaacggc cacgagttcg
     7501 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
     7561 tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag ttccagtacg
     7621 gctccaaggt gtacgtgaag caccccgccg acatccccga ctacaagaag ctgtccttcc
     7681 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga
     7741 cccaggactc ctccctgcag gacggctcct tcatctacaa ggtgaagttc atcggcgtga
     7801 acttcccctc cgacggcccc gtaatgcaga agaaaactat gggctgggag gcctccaccg
     7861 agcgcctgta cccccgcgac ggcgtgctga agggcgagat ccacaaggcc ctgaagctga
     7921 aggacggcgg ccactacctg gtggagttca agtccatcta catggccaag aagcccgtgc
     7981 agctgcccgg ctactactac gtggactcca agctggacat cacctcccac aacgaggact
     8041 acaccatcgt ggagcagtac gagcgcgccg agggccgcca ccacctgttc ctgactcacg
     8101 gtatggacga attgtacaag tcgaagctgt aagcttgtgc ttctaaggta tgagtcgcaa
     8161 aattgttttt tatttttggt cttgagtcta atatgctcgc agctcttgcg ttgtatatgg
     8221 tcgttgtcgc gtattttctg ttgtattaaa agatcaaacg agatcaaggg atggctcgcg
     8281 ggctgtctct cgcactagga ggaagaatgc ctgaaaaagg aactttgatt ttagctgtgg
     8341 aatagagatg gcttgtttga ggacgcttgt cgcttggcgc agggacttga atggcagctt
     8401 gtggaaaccg aaggcgagaa aagtcgacgg atactgtacg tggttctatt gccagtgcgg
     8461 tggaagcttc cttctcatat agttcaatcc ttctttgaat ctgtttgttt catatttgga
     8521 ctgtttcatt ctctgcttgc gcattctcat cttcgagaac gactgcaggg attgttggtt
     8581 ctgtggagct gatgagcgcg ccttgaccac ccttgttctt gttttgctct tttgttctca
     8641 tttaacccgt ttctcccttc caaccctttg accttgcaac attgtctccc agcgcgttgc
     8701 caaagcgaac ttgatatcag tatagtatga ccaagtagtc taccaaaata aattttagta
     8761 cagtattgct agttatacaa gataaatttt gtacaacaat gattatgcag agctggtatt
     8821 ctgtaaggag taaattcatg caggtacaga gcgtccacgt caggcacacg cgctttagtg
     8881 ctgggtacac attatgctac ctttggggca aggtgctatt ctaggtaggg tatgcctagc
     8941 aatgccatga tcttatgacc tcagtaatct gaccttggat ttaagatcgg agttctcaaa
     9001 ccctgcgcgc tcagagaatt cgcatgcgga gactttagac atacctctcg ttgccgacaa
     9061 atcggcattc cgtttcggtc acggacgtaa gagttcaaca aagccgctca cctgtcccgt
     9121 tcggcgcttg agtttccact tcaaaagcac aaccgctccg atatttccat tgccaccgac
     9181 caacaaaagg ttcatatcgc gcaatagtcg tccaactctc actccgcccg cactcgatgg
     9241 cgaaggatgg aagaatatcc gagcggcatc ggataaagag atctggttct tttcgtgaag
     9301 taacgtaggg aaccgacgga aactgtagag ctataggaat ttagtatatc agaagtcaat
     9361 tgagagaagt gtatagttag taactcactt gtgccaacag gtatttccaa ccgttcttgt
     9421 tcaaatttcg tcaagtcacc atctctatgt gcaaaactgg atcatacaac agctcttatt
     9481 gtagctgcat ctcgctctca gagatcatcc tatcttcgtc gtcatccacc aacacaggtg
     9541 ggagtgctag ttgatccatg ctgaggtcaa gggacacaga cggaatgggt gtagaacgcc
     9601 tcaaaacagg taaggtatca ggatcggttg tccaggggga atccaaacca ggttccacct
     9661 tgattcagag aataagtatg acgtattctt ggcaatgttt aagcttgaga tgagccgatg
     9721 tcaaactggg agacatgatg aaggaggaaa tgttgctttg gtgggggaga cactataaat
     9781 accaaagagt gaatggttga taccatttgg ggaaggagtg tctatcgcag tctctgcaga
     9841 cggcatatcg gatagcctgc aggcgcttgt ggaggatgca catgtgaccg agggacacga
     9901 agtgatccgt ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa
     9961 aagagcgttt attagaataa tcggatattt aaaagggcgt gaaaaggttt atccgttcgt
    10021 ccatttgtat gtgccagccg cctttgcgac gctcaccggg ctggttgccc tcgccgctgg
    10081 gctggcggcc gtctatggcc ctgcaaacgc gccagaaacg ccgtcgaagc cgtgtgcgag
    10141 acaccgcggc cgccggcgtt gtggatacct cgcggaaaac ttggccctca ctgacagatg
    10201 aggggcggac gttgacactt gaggggccga ctcacccggc gcggcgttga cagatgaggg
    10261 gcaggctcga tttcggccgg cgacgtggag ctggccagcc tcgcaaatcg gcgaaaacgc
    10321 ctgattttac gcgagtttcc cacagatgat gtggacaagc ctggggataa gtgccctgcg
    10381 gtattgacac ttgaggggcg cgactactga cagatgaggg gcgcgatcct tgacacttga
    10441 ggggcagagt gctgacagat gaggggcgca cctattgaca tttgaggggc tgtccacagg
    10501 cagaaaatcc agcatttgca agggtttccg cccgtttttc ggccaccgct aacctgtctt
    10561 ttaacctgct tttaaaccaa tatttataaa ccttgttttt aaccagggct gcgccctgtg
    10621 cgcgtgaccg cgcacgccga aggggggtgc ccccccttct cgaaccctcc cggcccgcta
    10681 acgcgggcct cccatccccc caggggctgc gcccctcggc cgcgaacggc ctcaccccaa
    10741 aaatggcagc gctggccaat tcccgagtgc gcggaacccc tatttgttta tttttctaaa
    10801 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
    10861 gaaaaaggaa gagtatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa
    10921 aataccgctg cgtaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg
    10981 gagaaaatga aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg
    11041 atgtggaacg ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg
    11101 tcctgcactt tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg
    11161 tcctttgctc ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg
    11221 cggagtgcat caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct
    11281 tagacagccg cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt
    11341 gcgaaaactg ggaagaggac actccattta aagatccgcg cgagctgtat gattttttaa
    11401 agacggaaaa gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca
    11461 tctttgtgaa agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg
    11521 acaagtggta tgacattgcc ttctgcgtcc ggtcgctcag ggaggatatc ggggaagaac
    11581 agtatgtcga gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat
    11641 attatatttt actggatgaa ttgttttagc tgtcagacca agtttactca tatatacttt
    11701 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    11761 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    11821 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    11881 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    11941 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
    12001 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    12061 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    12121 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    12181 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    12241 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    12301 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    12361 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    12421 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggcagatcct agatgtggcg
    12481 caacgatgcc ggcgacaagc aggagcgcac cgacttcttc cgcatcaagt gttttggctc
    12541 tcaggccgag gcccacggca agtatttggg caaggggtcg ctggtattcg tgcagggcaa
    12601 gattcggaat accaagtacg agaaggacgg ccagacggtc tacgggaccg acttcattgc
    12661 cgataaggtg gattatctgg acaccaaggc accaggcggg tcaaatcagg aataagggca
    12721 cattgccccg gcgtgagtcg gggcaatccc gcaaggaggg tgaatgaatc ggacgtttga
    12781 ccggaaggca tacaggcaag aactgatcga cgcggggttt tccgccgagg atgccgaaac
    12841 catcgcaagc cgcaccgtca tgcgtgcgcc ccgcgaaacc ttccagtccg tcggctcgat
    12901 ggtccagcaa gctacggcca agatcgagcg cgacagcgtg caactggctc cccctgccct
    12961 gcccgcgcca tcggccgccg tggagcgttc gcgtcgtctt gaacaggagg cggcaggttt
    13021 ggcgaagtcg atgaccatcg acacgcgagg aactatgacg accaagaagc gaaaaaccgc
    13081 cggcgaggac ctggcaaaac aggtcagcga ggccaagcag gccgcgttgc tgaaacacac
    13141 gaagcagcag atcaaggaaa tgcagctttc cttgttcgat attgcgccgt ggccggacac
    13201 gatgcgagcg atgccaaacg acacggcccg ctctgccctg ttcaccacgc gcaacaagaa
    13261 aatcccgcgc gaggcgctgc aaaacaaggt cattttccac gtcaacaagg acgtgaagat
    13321 cacctacacc ggcgtcgagc tgcgggccga cgatgacgaa ctggtgtggc agcaggtgtt
    13381 ggagtacgcg aagcgcaccc ctatcggcga gccgatcacc ttcacgttct acgagctttg
    13441 ccaggacctg ggctggtcga tcaatggccg gtattacacg aaggccgagg aatgcctgtc
    13501 gcgcctacag gcgacggcga tgggcttcac gtccgaccgc gttgggcacc tggaatcggt
    13561 gtcgctgctg caccgcttcc gcgtcctgga ccgtggcaag aaaacgtccc gttgccaggt
    13621 cctgatcgac gaggaaatcg tcgtgctgtt tgctggcgac cactacacga aattcatatg
    13681 ggagaagtac cgcaagctgt cgccgacggc ccgacggatg ttcgactatt tcagctcgca
    13741 ccgggagccg tacccgctca agctggaaac cttccgcctc atgtgcggat cggattccac
    13801 ccgcgtgaag aagtggcgcg agcaggtcgg cgaagcctgc gaagagttgc gaggcagcgg
    13861 cctggtggaa cacgcctggg tcaatgatga cctggtgcat tgcaaacgct agggccttgt
    13921 ggggtcagtt ccggctgggg gttcagcagc ccctgctcgg atctgttgga ccggacagta
    13981 gtcatggttg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg
    14041 agctgttggc tggctggtgg caggatatat tgtggtgtaa acaaattgac gcttagacaa
    14101 cttaataaca cattgcggac gtttttaatg tactggggtt gaacactct
//
