LOCUS       sequence-30630-e         764 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Jones RG, Parsons M, Bonnard M, Chan VS, Yeh WC, Woodgett JR, Ohashi
            PS
  TITLE     Protein kinase B regulates T lymphocyte survival, nuclear factor 
            kappaB activation, and Bcl-X(L) levels in vivo.
  JOURNAL   J Exp Med. 2000 May 15. 191(10):1721-34.
  PUBMED    10811865
REFERENCE   2  (bases 1 to 764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Med. 
            2000 May 15. 191(10):1721-34."
COMMENT     Sequence Label: GAG-PKB-pcDNA3
FEATURES             Location/Qualifiers
     source          1..764
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             206..622
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     552..574
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     590..606
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctt ggtaccgagc tcggatccac tagtaacggc cgccagtgtg
      121 ctggaattcg gatcagcttg tctgatgcag catcgttctg tgttgtctct gtctgactgt
      181 gtttctgtat ttgtctgaaa atatgggcca gactgttacc actcccttaa gtttgacctt
      241 aggtcactgg aaagatgtcg agcggatcgc tcacaaccag tcggtagatg tcaagaagag
      301 acgttgggtt accttctgct ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga
      361 cggcaccttt aaccgagacc tcatcaccca ggttaagatc aaggtctttt cacctggccc
      421 gcatggacac ccagaccagg tcccctacat cgtgacctgg gaagccttgg cttttgaccc
      481 ccctccctgg gtcaagccct ttgtacaccc taagccttcg cctcctcttc ctccatccgc
      541 cccgtctctc ccccttgaac ctcctcgttc gaccccgcct cgatcctccc tttatccagc
      601 cctcactcct tctctaggcg ccaaacctaa acctcaagtt ctttctgaca gtggggggcc
      661 gctcatcgac ctacttacag aagacccccc gccttatagg gacccaagac cacccccttc
      721 cgacagggac ggaaatggtg gagaagcgac ccctgcggga gagg
//
