LOCUS       sequence-30634-e         347 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Yan M, Dai T, Deak JC, Kyriakis JM, Zon LI, Woodgett JR, Templeton 
            DJ
  TITLE     Activation of stress-activated protein kinase by MEKK1 
            phosphorylation of its activator SEK1.
  JOURNAL   Nature.   . 372(6508):798-800.
  PUBMED    7997270
REFERENCE   2  (bases 1 to 347)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature.   .
            372(6508):798-800."
COMMENT     Sequence Label: HA SEK AL pcDNA3.1 zeo (+) - SEK1 (A-L)
FEATURES             Location/Qualifiers
     source          1..347
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     123..139
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     124..140
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             188..214
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagc gtttaaactt aagcttggta ccgggccccc
      121 cctcgaggtc gacggtatcg ataagcttga tatcgaattc ctgcagcccc catctagagc
      181 caccatgtac ccatacgatg ttccagatta cgctggaatt cgaaccatgg agggtaagcg
      241 caaagcactg aagttgaatt ttgcaaatcc acctgtcaaa tcgacagcac ggtttaccct
      301 gaatcctaat actacaggag tccagaaccc acacatagag agactga
//
