LOCUS       sequence-306412-       10504 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TagRFP-T^SEC^3xMyc vector with ccdB sites for cloning homology 
            arms..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10504)
  TITLE     FP-SEC vectors for homologous recombination in C. elegans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10504)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pDD286 (TagRFP^SEC^3xMyc)
FEATURES             Location/Qualifiers
     source          1..10504
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             661..966
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             1923..1943
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     protein_bind    complement(1961..1994)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             2004..2030
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     terminator      complement(3848..4171)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    6089..6113
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6116..7141
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6116..7141
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(7142..7166)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7519..7552)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             7571..7660
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     CDS             complement(7705..8010)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(8283..8299)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8283..8299)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8296..8318)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8307..8323
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8331..8337,8338..8355,8356..8361))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8376..8397
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8514..8531)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8685..9273)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8765..8784)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9444..10235,10236..10304))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10067..10086
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10305..10409)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10477..10495
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtcgccg gcactagtta cccgaagtat
      421 gtcaaaaaga ggtatgctat gaagcagcgt attacagtga cagttgacag cgacagctat
      481 cagttgctca aggcatatat gatgtcaata tctccggttc ggtaagcaca accatgcaga
      541 atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg
      601 aggtcgcccg gtttattgaa atgaacggct cttttgccga cgagaacagg ggctggtgaa
      661 atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta
      721 cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt
      781 ctgctgtcag ataaagtccc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc
      841 tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg
      901 gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga
      961 atataaatgt caggctccct tatacacagc cagtctgcag gtcgacctag ggagcatcgg
     1021 gagcctcagg agcatcgatg gtctccaagg gagaggagct catcaaggag aacatgcaca
     1081 tgaagctcta catggaggga accgtcaaca accaccactt caagtgcacc tccgagggag
     1141 agggaaagcc atacgaggga acccaaacca tgcgtatcaa ggtaagttta aacatatata
     1201 tactaactaa ccctgattat ttaaattttc aggtcgtcga gggaggacca ctcccattcg
     1261 ccttcgacat cctcgccacc tccttcatgt acggatcccg taccttcatc aaccacaccc
     1321 aaggaatccc agacttcttc aagcaatcct tcccagaggg attcacctgg gagcgtgtca
     1381 ccacctacga ggacggagga gtcctcaccg ccacccaaga cacctccctc caagacggat
     1441 gcctcatcta caacgtcaag gtaagtttaa acagttcggt actaactaac catacatatt
     1501 taaattttca gatccgtgga gtcaacttcc catccaacgg accagtcatg caaaagaaga
     1561 ccctcggatg ggaggccaac accgagatgc tctacccagc cgacggagga ctcgagggac
     1621 gtaccgacat ggccctcaag ctcgtcggag gaggacacct catctgcaac ttcaagacca
     1681 cctaccgttc caaggtaagt ttaaacatga ttttactaac taactaatct gatttaaatt
     1741 ttcagaagcc agccaagaac ctcaagatgc caggagtcta ctacgtcgac caccgtctcg
     1801 agcgtatcaa ggaggccgac aaggagacct acgtcgagca acacgaggtc gccgtcgccc
     1861 gttactgcga cctcccatcc aagctcggac acaagctcaa cggaatggac gagctctaca
     1921 aggagaatct gtactttcaa tccggaaagg taagtttaaa ataacttcgt ataggatact
     1981 ttatacgaag ttattttcag ggatacccat acgacgtccc agactacgcc taaggatgat
     2041 cgacgccaac gtcgttgaat tttcaaattt taaatactga atatttgttt tttttcctat
     2101 tatttattta ttctctttgt gttttttttc ttgctttcta aaaaattaat tcaatccaaa
     2161 tctaaacatt tttttttctc tttccgtctc ccaattcgta ttccgctcct ctcatctgaa
     2221 cacaatgtgc aagtttattt atcttctcgc tttcatttca ttaggacgtg gggggaattg
     2281 gtggaagggg gaaacacaca aaaggatgat ggaaatgaaa taaggacaca caatatgcaa
     2341 caacattcaa ttcagaaata tggaggaagg tttaaaagaa aacataaaaa tatatagagg
     2401 aggaaggaaa actagctgcc ctcgattgtc tatcttccat tctcaccaag tttcaacaac
     2461 tttattcagt cattcttctc tggttcattt cccaaccaat cttcccgaat aaagcccatc
     2521 tgtcctttcg gccacattcc tcacctacat ccgtactcct tatctcccgt tcttatccag
     2581 tcttttatcg tctctgcgtc tcgcacactg tctcgtcggt aatgtaaaaa aaaaacaaaa
     2641 aaaacttgaa aacttgcgga aagatttcct aataaaagga gacgaatccg gtaggaaatc
     2701 gacaagtctc tgcttctcga agactaggtg agttacattt ctttggccga attattttga
     2761 aatcctaaat ttgataattt cagagatgtc tgtaaaactt gcgtgttatg tgacggcttc
     2821 ggtcactgtc gccactctta tggtatgctt catgaccatg tcaaccatct actcggaggt
     2881 tgatggattc agagagaagc ttgataccga gatgaatgtg ttcagagtga gttaaattat
     2941 attttgaatt ttaaataatt tttaattttc tagcaatcta ccaatggatt gtggaaggac
     3001 atagttgtca tcggaagatc tagcaagcgt gtccgttgtc aatatgaaga gaccaacgct
     3061 accccaactc cacatgctga tggatcccca tctgctccac caggtcaacc accagcagtt
     3121 ccaccagtct tcaaccagcc aaagactcca aatggagcca atggaaatgg accaacctgc
     3181 aactgcaatg ctgataacaa gtgcccagct ggaccatccg gaccaaaggg agttccagga
     3241 gttccaggac tcgacggagt tccaggactt gacggtgttc caggagttgg agctgatgat
     3301 atcgctccac aacgcgagtc tgtcggatgc ttcacttgcc cacaaggacc agttggacca
     3361 ccaggagctc ttggaagacc aggaccacgt ggacttccag gaccaagagg acaaaatgga
     3421 aacccaggaa gagatggaca accaggacat ccaggagagc aaggatcatc cggtcaaatc
     3481 ggaaagatcg gagagccagg accaccagga gagaagggac gcgacgccga gcatccaatc
     3541 ggaagaccag gaccaaaggg accaagagga gatcaaggac caacaggacc agctggacag
     3601 aacggtcttc acggaccacc aggagagcca ggaaccgttg gaccagaagg accatctgga
     3661 aagcaaggac gtcaaggacc agacggaacc cagggagaga ctggaccaga cggaagacca
     3721 ggaaaggatg ccgagtactg ccagtgccca gacaagtctc caccatcaga ggctgtcaac
     3781 gccaaccgtg gatacagaaa tatctaaatt gttggtgttt tctaataaaa tatttgagat
     3841 tcagtgagac ttttttcttg gcggcacaat aaagtttcct ctttttgttg ggtcactcaa
     3901 cgaacacaat acaaatattt gagcgctcgc atttttctca acctttcggg tttaaagcac
     3961 ttcaaaatgg aattgaagag agattgtgaa agtacatcag acagaatcac attttgatag
     4021 cattcacttc actcagatgc aaacttttca gtgactataa aaagtcataa aataataaca
     4081 ttaaaaaagc aaataaatta gcgagagaat tttttgacaa aaagaaagaa gagtgataga
     4141 gaagaaggga atgcttgaaa ggattttgca tttatcttaa tcaccgtctt cgagaagacg
     4201 aaccatggcg ccagtttcag aatccaagtt tctaatatag ttcatcacaa tgttgacgtt
     4261 ggtccaacct ccggcttgca taatctctgg gatggaaact ccagcgcgag ccatgtcacg
     4321 ggcagctccg acacgggcgg aatgtccaga ccaagccaag taacgttgtc cggaatcatc
     4381 cttggctccg tagatgagac ggtgggtagc ttcgaagatt cctgaaaatt tattttcagc
     4441 ttattcttag ctaatatgtt gtttaaactt accttcaaga gctcgagtcg agagttgtga
     4501 tgttgcactc ggggcagcaa ctccgttctt gcgaacacga cagaacaggt agttgtttgg
     4561 atcatcggcg actccggaaa ccgaaatcca gcgctcaacg agcttggtaa ctccaaggga
     4621 gagcgctttt tcgactccgg cagtcgagac cagcgttttt gtacgtccga tgtggatgag
     4681 cattcttcct ccgtctgtgc gactaatatc cttgacgcga atgcgggcaa tttcggcgat
     4741 acggagaagt gtgttgtaag caattccgag gaaggcgagg tttcggatgt cctggcaacg
     4801 atcggaattt tccataagag aacggacctg atcgaagtcg gttctgaaaa tttcgtcaag
     4861 tcttaggatt gaatacatta atatttaaac ttacctttcg aaggcgaggg cttgcttggc
     4921 tctctctcca gcatcaacat tttccttgcg gatacgtctc attacgagag atacggcatt
     4981 agaatctgat gggcgtggaa gtccagatcg gcgatggagc atattgagct gtccgagatg
     5041 ttgttggatc gtcttcaccg ccaatccacg agcttggagg tagagcaagt agtcacgaac
     5101 gtcttctggc tcggctggga accacttgcg gttgttaagc ttgcaccagg cagcccaaga
     5161 acggcacact gagagaagca tcttccaggt gtgctcggag aaggcttgtc tgtcacggaa
     5221 catatccatg aggttcttgc gaacttcatc acttgtcgcg tcaactggaa gtgctggaag
     5281 attttgatga acagtgagaa gatttgacat attttcgaag ttttttagat gcactagaac
     5341 aaagcgtgtt ggcttcctct gagcccgctt tccttatata cccgcattct gcagccttac
     5401 agaatgttct agaaggtcct agatgcattc gtttgaaaat actcccggtg ggtgcaaaga
     5461 gacgcagacg gaaaatgtat ctgggtctct ttattgtgta cactactttt ccatgtaccg
     5521 aatgtgagtc gccctccttt tgcaacaagc agctcgaatg ttctagaaaa aggtggaaaa
     5581 tagtataaat accgttgaaa ataaataccg aacaacattt gctctaattg tgaaattaga
     5641 aatcttcaaa ctataatcat gtcactttac cactatttcc gtccagctca acgttccgtt
     5701 tttggtgatc atttttgctt tcgtcgtaaa tctacacacg cgtctcttcc gtgcgagagt
     5761 ccaagccagc agccaaattc gttgactgag tattcaacgt ttatacgttg tcggcaacga
     5821 gaaataggaa aatgcatcgg gaaatgttct tttttcgatt ttttccaagg ttttgacaaa
     5881 ttttaccacg aattttgcta tgttttcaat taaaaaatat gttattcaac tgtttctatg
     5941 aggaaaataa ggctttgcat gtaattttct tattcagcat aatttttaat taatttgaat
     6001 tttctgtcct aacgtttatt ttgttttctt ggttatgact gatctgaaat taatttttga
     6061 attttaaggt aatatgtcag gcggtgccgc aagtttgtac aaaaaagcag gctccatgaa
     6121 aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt
     6181 ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg
     6241 agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta
     6301 tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga
     6361 attcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga
     6421 cctgcctgaa accgaactgc ccgctgttct gcagccggtc gcggaggcca tggatgcgat
     6481 cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg
     6541 tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg
     6601 gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat
     6661 gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa
     6721 caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt
     6781 cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat
     6841 ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct
     6901 ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa
     6961 tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg
     7021 gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt
     7081 agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaaggaata
     7141 gacccagctt tcttgtacaa agtgggtcca attactcttc aacatcccta catgctcttt
     7201 ctccctgtgc tcccaccccc tatttttgtt attatcaaaa aacttctctt aatttctttg
     7261 ttttttagct tcttttaagt cacctctaac aatgaaattg tgtagattca aaaatagaat
     7321 taattcgtaa taaaaagtcg aaaaaaattg tgctccctcc ccccattaat aataattcta
     7381 tcccaaaatc tacacaatgt tctgtgtaca cttcttatgt tttttacttc tgataaattt
     7441 ttttgaaaca tcatagaaaa aaccgcacac aaaatacctt atcatatgtt acgtttcagt
     7501 ttatgaccgc aatttttaat aacttcgtat aggatacttt atacgaagtt attttcaggg
     7561 agccggatct gaacaaaaac ttatttctga agaggatctt gagcaaaagc tcatctccga
     7621 ggaggacctc gagcagaagt tgatcagcga ggaagacttg ccggctcgac ctgcagactg
     7681 gctgtgtata agggagcctg acatttatat tccccagaac atcaggttaa tggcgttttt
     7741 gatgtcattt tcgcggtggc tgagatcagc cacttcttcc ccgataacgg agaccggcac
     7801 actggccata tcggtggtca tcatgcgcca gctttcatcc ccgatatgca ccaccgggta
     7861 aagttcacgg gggactttat ctgacagcag acgtgcactg gccaggggga tcaccatccg
     7921 tcgcccgggc gtgtcaataa tatcactctg tacatccaca aacagacgat aacggctctc
     7981 tcttttatag gtgtaaacct taaactgcat ttcaccagcc cctgttctcg tcggcaaaag
     8041 agccgttcat ttcaataaac cgggcgacct cagccatccc ttcctgattt tccgctttcc
     8101 agcgttcggc acgcagacga cgggcttcat tctgcatggt tgtgcttacc gaaccggaga
     8161 tattgacatc atatatgcct tgagcaactg atagctgtcg ctgtcaactg tcactgtaat
     8221 acgctgcttc atagcatacc tctttttgac atacttcggg ttcctaggaa atcgataaca
     8281 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     8341 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     8401 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     8461 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     8521 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     8581 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     8641 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     8701 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     8761 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     8821 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     8881 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     8941 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     9001 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     9061 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     9121 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     9181 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     9241 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     9301 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     9361 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     9421 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     9481 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     9541 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     9601 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     9661 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     9721 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     9781 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     9841 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     9901 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     9961 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    10021 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    10081 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    10141 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    10201 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    10261 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    10321 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    10381 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
    10441 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
    10501 cgtc
//
